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  4. Sheep adrenocorticotropic hormone (ACTH) ELISA kit

Sheep adrenocorticotropic hormone (ACTH) ELISA kit

  • 中文名称：
    绵羊促肾上腺皮质激素(ACTH)酶联免疫试剂盒
  • 货号：
    CSB-EQ027618SH
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Sheep ACTH ELISA Kit was designed for the quantitative measurement of Sheep ACTH protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.097 pg/mL-400 pg/mL and the sensitivity is 0.097 pg/mL.

  • 缩写：
    ACTH
  • 种属：
    Ovis aries (Sheep)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.097 pg/mL-400 pg/mL
  • 灵敏度：
    0.097 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 82-89  
    1:2 Average % 91  
    Range % 88-95  
    1:4 Average % 87  
    Range % 83-95  
    1:8 Average % 100  
    Range % 95-105  
  • 回收率：
    The recovery of sheep ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-103  
    EDTA plasma (n=4) 89 87-92  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.175 0.177 0.176    
    100 0.254 0.268 0.261    
    25 0.335 0.353 0.344    
    6.25 0.582 0.598 0.590    
    1.56 0.913 0.934 0.924    
    0.39 1.461 1.498 1.480    
    0.097 1.751 1.659 1.705    
    0 2.031 1.957 1.994    
  • 数据处理：
  • 货期：
    3-5 working days

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