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  4. Fish adrenocorticotropic hormone (ACTH) ELISA kit

Fish adrenocorticotropic hormone (ACTH) ELISA kit

  • 中文名称：
    鱼促肾上腺皮质激素(ACTH)酶联免疫试剂盒
  • 货号：
    CSB-E15926Fh
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

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  • 产品描述：

    This Fish ACTH ELISA Kit was designed for the quantitative measurement of Fish ACTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 75 pg/mL-1200 pg/mL and the sensitivity is 75 pg/mL.

  • 缩写：
    ACTH
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    75 pg/mL-1200 pg/mL
  • 灵敏度：
    75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 105  
    Range % 94-109  
    1:2 Average % 92  
    Range % 84-97  
    1:4 Average % 101  
    Range % 98-105  
    1:8 Average % 95  
    Range % 84-98  
  • 回收率：
    The recovery of fish ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 82-97  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1200 0.149 0.152 0.151    
    600 0.300 0.288 0.294    
    300 0.477 0.491 0.484    
    150 0.871 0.889 0.880    
    75 1.835 1.887 1.861    
  • 数据处理：
  • 货期：
    3-5 working days

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