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Sheep Somatotropin(GH1) ELISA kit

  • 中文名称：
    绵羊生长激素(GH1)酶联免疫试剂盒
  • 货号：
    CSB-EL009407SH
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Sheep GH1 ELISA Kit was designed for the quantitative measurement of Sheep GH1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 ng/mL-40 ng/mL and the sensitivity is 0.675 ng/mL.

  • 别名：
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Ovis aries (Sheep)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.5 ng/mL-40 ng/mL
  • 灵敏度：
    0.675 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep GH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-101  
    1:2 Average % 93  
    Range % 87-98  
    1:4 Average % 96  
    Range % 95-102  
    1:8 Average % 95  
    Range % 89-99  
  • 回收率：
    The recovery of sheep GH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-102  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    40 0.173 0.175 0.174    
    20 0.308 0.312 0.310    
    10 0.639 0.644 0.642    
    5 1.401 1.408 1.405    
    2.5 2.261 2.265 2.263    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 基因功能参考文献：
    1. Association analyses showed that both g.498G>C and g.616G>A GH polymorphisms were significantly associated with several growth traits (at P < 0.01 or P < 0.05) in three investigated sheep breeds. PMID: 27706626
    2. The effect leptin, orexin-A, and ghrelin on the secretion of luteinizing hormone (LH) and growth hormone by adenohypophyseal explants obtained from normally fed or fasted ewes during the breeding and nonbreeding seasons. PMID: 24906931
    3. The results showed that the growth hormone genotypes are associated with estimated breeding values of growth traits. PMID: 21328104
    4. The simultaneous increase of expression of hypothalamic NPY suggests NPY involvement in the regulation of psychoemotional stress through the somatotrophic axis in the female lambs. PMID: 20675289
    5. These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
    6. Results show that in comparison to the product of the ovine growth hormone (GH)2-N gene copy, the postulated product of the GH2-Z gene copy, the G9R/G63S mutein, has a higher affinity to the growth hormone receptor and elevated biological activity. PMID: 17850798
    7. Results describe thet pulsatility of cerebrospinal fluid and plasma ghrelin levels in relation to growth hormone secretion and food intake in the sheep, and the effects of exogenous ghrelin on GH and feeding behavior. PMID: 18673413
    8. study has demonstrated for the first time that ghrelin differentially modulates growth hormone secretory response to growth hormone-releasing hormone according to feeding states in ruminant animals PMID: 19342191

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：

    KEGG: oas:443329

    UniGene: Oar.641