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  4. Rat soluble E-selectin,sE-selectin ELISA Kit

Rat soluble E-selectin,sE-selectin ELISA Kit

  • 中文名称：
    大鼠可溶性E选择素(sE-selectin)酶联免疫试剂盒
  • 货号：
    CSB-E07996r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat soluble E-selectin (sE-selectin) ELISA Kit is a powerful tool for immunology researchers to measure the levels of sE-selectin in a variety of sample types from Rattus norvegicus (Rat).

    This ELISA kit has a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL, providing accurate and reliable results for even low levels of E-selectin. The assay time is 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.

    The sE-selectin ELISA Kit utilizes a sandwich assay principle, where the E-selectin antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.

    This ELISA kit is suitable for a range of sample types, including serum, plasma, cell culture supernates, and tissue homogenates, making it a versatile solution for researchers in the field of immunology. It has been cited in over 9 publications, highlighting its reliability and utility in research.

  • 别名：
    Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %92
    Range %88-108
    1:10Average %94
    Range %91-98
    1:20Average %96
    Range %89-100
    1:40Average %84
    Range %80-88
  • 回收率：
    The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9993-105
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.746 2.645 2.696 2.542
    25001.976 1.875 1.926 1.772
    12501.228 1.204 1.216 1.062
    6250.782 0.761 0.772 0.618
    3120.522 0.511 0.517 0.363
    1560.363 0.352 0.358 0.204
    780.290 0.285 0.288 0.134
    00.154 0.153 0.154 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
  • 基因功能参考文献：
    1. The e-selectin/biliverdin reductase pathway participates in the inhibitory process of hypoxia in pulmonary artery endothelial cell apoptosis which is mediated by the mitochondrial-dependent apoptosis pathway. PMID: 27033411
    2. Decrease in uterine pinopodes and L-selectin ligand MECA-79 expression during uterine receptivity period by testosterone may cause failure of blastocyst implantation. PMID: 24966906
    3. E-selectin blockade similarly inhibits the migration of activated CCR4(negative) and CCR4(positive) CD4 T cells to dermal inflammation. PMID: 22664869
    4. The expression of E-selectin is significantly increased in lung tissue of ovariectomized rats, an effect prevented by estradiol but not by progesterone treatment. PMID: 20735828
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Selectin/LECAM family
  • 数据库链接：