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  4. Rat advanced glycation end products,AGEs ELISA Kit

Rat advanced glycation end products,AGEs ELISA Kit

  • 中文名称：
    大鼠晚期糖基化终末产物(AGEs)酶联免疫试剂盒
  • 货号：
    CSB-E09413r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat AGEs ELISA Kit was designed for the quantitative measurement of Rat AGEs protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9 μg/mL-250 μg/mL and the sensitivity is 0.97 μg/mL .

  • 缩写：
    AGEs
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.9 μg/mL-250 μg/mL
  • 灵敏度：
    0.97 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %86
    Range %84-95
    1:10Average %95
    Range %91-100
    1:20Average %85
    Range %81-89
    1:40Average %93
    Range %89-96
  • 回收率：
    The recovery of rat AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-96
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2AverageCorrected
    2502.194 2.286 2.240 2.084
    1251.764 1.789 1.777 1.621
    62.51.270 1.256 1.263 1.107
    31.20.921 0.975 0.948 0.792
    15.60.455 0.465 0.460 0.304
    7.80.297 0.308 0.303 0.147
    3.90.248 0.233 0.241 0.085
    00.161 0.150 0.156 　
  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

我想从贵公司订购“rat advanced glycation end products (AGEs) ELISA Kit (#CSB-E09413r)”，但我想了解以下信息： Q1,是否有数据说明为什么我们可以根据宿主物种选择AGEs Elisa试剂盒？由于AGEs没有特异性表位，我认为宿主物种并不是确定AGEs抗体特异性的关键因素。 Q2.如果我使用大鼠AGEs ELISA kit来分析小鼠组织样本，很难保证该试剂盒的特异性或数据质量问题吗？

A:
A1：AGEs是一系列物质的通称，而不是一个单一的物质。它们具有相同的抗原决定簇。我们之所以有不同物种试剂盒，是不同物种的试剂盒采用原料特性不同，抗基质干扰、适用性也不同。这个CSB-E09413r试剂盒（大鼠AGEs ELISA试剂盒）应用了小鼠单克隆抗体和山羊多克隆抗体。而这个CSB-E09414m试剂盒（小鼠AGEs ELISA试剂盒）应用了大鼠单克隆抗体和山羊多克隆抗体。因此我们建议用户使用相应物种的ELISA试剂盒进行检测。 A2：这个试剂盒与人和小鼠AGEs重组蛋白没有交叉反应。我们用这个试剂盒测试了小鼠血样，没有检测到任何数值。我们不建议进行跨物种检测。