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  4. Mouse advanced glycation end products,AGEs ELISA Kit

Mouse advanced glycation end products,AGEs ELISA Kit

  • 中文名称：
    小鼠晚期糖基化终末产物(AGEs)酶联免疫试剂盒
  • 货号：
    CSB-E09414m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse AGEs ELISA Kit was designed for the quantitative measurement of Mouse AGEs protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL .

  • 缩写：
    AGEs
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 μg/mL-10 μg/mL
  • 灵敏度：
    0.039 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %82-91
    1:2Average %97
    Range %92-103
    1:4Average %99
    Range %94-110
    1:8Average %95
    Range %87-100
  • 回收率：
    The recovery of mouse AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9384-99
    EDTA plasma (n=4)9792-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µg/mlOD1OD2AverageCorrected
    101.958 1.900 1.929 1.779
    51.583 1.602 1.593 1.443
    2.51.160 1.154 1.157 1.007
    1.250.754 0.739 0.747 0.597
    0.6250.471 0.434 0.453 0.303
    0.3120.342 0.319 0.331 0.181
    0.1560.253 0.239 0.246 0.096
    00.152 0.148 0.150 　
  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

Regarding your ELISA kit CSB-E09414m, could you please advise on the material you used for the standard in this kit? What AGE is this kit detecting?

A:
Thanks for your inquiry.
This kit detects total AGEs. The standard is polymer.
There are some protective components in the standard, but sorry we can not disclose the specific components.
Pls let me know if you have any further questions. Thank you.