Rat Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.

1. This study showed that voluntary exercise, but not forced exercise, could significantly increase Flk-1 expression in the hippocampus and cerebellum and Flk-1 expression was elevated in astrocytes. PMID: 28958753
2. Elevation of lung VEGF and VEGFR-1 expressions after lung contusion (LC) alone was associated with healing of injured lung tissue. Expressions of VEGF, VEGFR-1, and VEGFR-2 were reduced after LC followed by hemorrhagic shock (LCHS) and LCHS/chronic stress, and injured lung tissue did not heal. PMID: 28457321
3. Results suggest that 10 % hypertonic saline could alleviate cerebral edema possibly through reducing the ischemia induced blood-brain barrier permeability as a consequence of inhibiting VEGF-VEGFR2-mediated down-regulation of ZO-1, claudin-5. PMID: 27733124
4. Hypoxia significantly increased protein levels of VEGF, KDR, Angiopoiein 1, and Tie2, which were reduced following Compound 49b treatment. These data strongly suggested that Compound 49b protected the retina against I/R-induced injury. This provides additional support for a role of beta-adrenergic receptor actions in the retina. PMID: 27439004
5. The activation of Akt by neuropilin 1 (NRP1) and vascular endothelial growth factor receptor 2 (VEGFR2) was important for ELC migration and following in vivo therapeutic outcomes PMID: 26509169
6. microwave radiation may induce blood brain barrier damage by activating the VEGF/Flk-1-ERK pathway. PMID: 25195697
7. a novel pathway wherein heparanse sequentially activates integrin beta1, HIF-2alpha, Flk-1, and p38MAPK/HSP27 with corresponding enhancement of angiogenesis PMID: 25754303
8. VEGF/VEGFR/K-ras signaling pathway might promote the occurrence and development of hepatocellular carcinoma cells through regulating expression of miRNA21, which has potential clinical value for the development of therapies for hepatocellular carcinoma PMID: 25730004
9. Our present findings support the hypothesis that VEGFA activates and causes an interaction between EPOR and VEGFR2 to contribute to pathological angiogenesis. PMID: 24630601
10. NOX4 may regulate VEGFR2-mediated intravitreal neovascularization through activated STAT3. PMID: 24623966
11. Data indicate the influence of hypoxia on VEGF-A signaling in bone marrow-derived endothelial progenitor cells (BM-EPCs) utilizing proteomic strategy to identify interacting downstream components of the combined VEGF receptor(s) signaling pathways. PMID: 24022223
12. Activation of the VEGFR-2 signal pathway provides neuroprotection for potassium deprivation in cerebellar granule neurons. PMID: 23826635
13. a novel mechanism of occludin Ser490 phosphorylation and ubiquitination downstream of VEGFR2 activation associated with early reperfusion injuty-induced vascular permeability. PMID: 24398936
14. These observations highlight the pivotal role that the eNOS-nitric oxide system plays in regulating the biologic response to VEGF within the glomerulus. PMID: 24063000
15. decursin may be a potent anti-angiogenic and anti-proliferative agent targeting the VEGFR-2 signaling pathway, which significantly inhibits diabetic retinal neovascularization. PMID: 23684887
16. Extensive network centered on VEGFR-2 signaling was essentially contributed to early recovery processes after muscle denervation. PMID: 23831211
17. our findings suggest that VEGF signaling through VEGFR-2 plays a critical regulatory role in protecting stressed hippocampal neurons from the damaging effects of an oxidative insult. blocked. PMID: 23732519
18. Data indicate that Rehmannia glutinosa extract (RGE) up-regulated the expression of angiogenesis-associated ligand/receptor, including CD133, VEGFR2 and SDF-1alpha/CXCR4. PMID: 23349848
19. These findings suggest that MSCs have a therapeutic potential in emphysematous rats by suppressing the inflammatory response, excessive protease expression, and cell apoptosis, as well as up-regulating VEGF, VEGF receptor 2, and TGFbeta-1 PMID: 22949406
20. Soy diet inhibited the ischemia-induced increase in cortical VEGF receptor (VEGFR)-2 protein expression observed 4 and 24 hours after stroke via HIF1alpha inhibition, although mRNA levels increased. PMID: 23456363
21. Brucine potently suppresses angiogenesis by targeting VEGFR2 activation and may be a viable drug candidate in anti-angiogenesis and anti-cancer therapies. PMID: 23348691
22. An interaction of the angiotensin II receptor subtype 2 (AT2-R) with vascular endothelial growth factor receptor 2 (VEGFR2) in the neonatal brain microvasculature that produces protective effects which are associated with injury tolerance. PMID: 22569153
23. Nicotine exposure caused a significant decrease in vascular endothelial growth factor receptor (VEGFR)-2 mRNA expression, compared with the level of the control rats on Postnatal Day 1. PMID: 22245234
24. VEGFR-2 expression in both brain tissue and cultured rat brain microvascular endothelial cells is enhanced by simvastatin in vivo and in vitro following traumatic brain injury. PMID: 21307798
25. The long form of Flk-1 is the predominant mediator of VEGF-A in the pathogenesis of diabetic retinopathy (DR) and can be significantly inhibited by the IVTA treatment. PMID: 22121831
26. VEGF and VEGF-R2 was expressed on the debris within the hematoma and bone trabeculae from days 1 to 28. PMID: 21928073
27. VEGF receptor 2 mRNA is significantly downregulated in endothelial progenitor cells from hyperaldosterone rats. PMID: 20116868
28. Radix Ginseng and Radix Notoginseng extract can up-regulate the protein and mRNA expressions of VEGFR-2 and HIF-1alpha and increase microvessel density in ischemic myocardium. PMID: 20550877
29. VEGFR2+PDGFRbeta+ circulating precursor cells participate in capillary restoration after hyperoxia acute lung injury PMID: 19426150
30. spontaneously hypertensive rat exhibited a decreased labeling of the extracellular, but not the intracellular, domain of VEGFR-2 along mesenteric microvessels PMID: 21418372
31. The expression of VEGFR2 in C6 gliomas was assessed by using a specific molecular probe with molecular magnetic resonance imaging. PMID: 20497492
32. VEGFR-2 is expressed in phrenic motor neurons and signals via extracellular signal-regulated (ERK) mitogen-activated protein kinases (ERK) and Akt. PMID: 21613481
33. PTK787/ZK222584 inhibits VEGFR and PDGFRbeta pathways in intussusceptive angiogenesis and has an effect on glomerular recovery from Thy1.1 nephritis PMID: 21435466
34. expression of erbB, VEGF and its receptors, and ER were determined at varying intensities at different sites of the mammary gland during pregnancy, lactation, and involution periods PMID: 20572782
35. Results show that VEGF and VEGFR2 are involved in the pathogenesis of neuropathic pain and VEGF primarily potentiates pain responses mediated by P2X2/3 receptor on DRG neurons. PMID: 20705122
36. Our findings show that VASP phosphorylation is affected by hypoxia and VEGFR2 inhibition suggesting a role for VASP in BBB permeability. PMID: 20599605
37. VEGFR-2 signaling may play a role in PlGF-mediated neuroprotection PMID: 20637183
38. farnesiferol C exerts antiangiogenic and antitumor activity and targets multiple aspects of VEGFR1 (Flt1) or VEGFR2 (Flk1) signaling cascades PMID: 20103598
39. expression of VEGF(164) and VEGFR2 mRNAs and VEGF protein were increased in association with retinopathy of prematurity model and older developmental age PMID: 20697002
40. The expression of VEGF, VEGFR1 and VEGFR2 in the asthmatic group were increased, and may be involved in angiogenesis and airway remodeling. PMID: 19575935
41. Increased expressions of VEGF and Flk-1 in the oxgen fluctuations-induced neovascularized retina suggested that VEGF and Flk-1 might play a critical role in the pathogenesis of retinopathy of prematurity. PMID: 17349140
42. These results indicate that the VEGF-VEGF receptor system is downregulated in puromycin aminonucleoside nephrosis (PAN), implying that it is not involved in the mechanism of proteinuria in PAN. PMID: 11744811
43. Renal ischemia-reperfusion increases endothelial VEGFR-2 without increasing VEGF or VEGFR-1 expression. PMID: 11967019
44. different combinations of vascular endothelial growth factor isoforms and their receptors regulate angiogenesis in the development of the masseter muscle PMID: 12208074
45. results show that the ovary is the main source of vascular endothelial growth factor(120) and vascular endothelial growth factor(164), which act through the vascular endothelial growth factor receptor-2 to increase vascular permeability PMID: 12399430
46. VEGF expression in chronic cyclosporine nephrotoxicity is increased by nitric oxide blockade and decreased by nitric oxide enhancement. VEGF probably exerted its effect via KDR/Flk-1 receptor. PMID: 12631117
47. differential expression of VEGF splicing isoforms along with its receptors may play an important role in the healing process after rat femoral drill-hole injury. PMID: 12753865
48. results suggest that Flt-1 and Flk-1 are expressed in the neurons with their individual time-dependent manners and regional distribution in the brain PMID: 12923895
49. KDR and MT1-MMP have roles in angiogenesis in spontaneous hypertensive rats PMID: 14766216
50. Mechanical stretch induces upregulation of the key tyrosine kinase receptors Flk-1, Tie-2, and Tie-1 in vascular endothelial cells PMID: 15548727

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Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Cell junction. Endoplasmic reticulum.

Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily

Expressed in the post-pubertal mammary glands.

KEGG: rno:25589

STRING: 10116.ENSRNOP00000066886

UniGene: Rn.88869