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  4. Rat UDP-glucumno-syltransferase 1(UGT1) ELISA Kit

Rat UDP-glucumno-syltransferase 1(UGT1) ELISA Kit

  • 中文名称：
    大鼠尿苷二磷酸葡萄糖醛酸转移酶1(UGT1)酶联免疫试剂盒
  • 货号：
    CSB-E13845r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat UDP-glucuronosyltransferase 1(UGT1) ELISA Kit is designed for reliable and accurate quantification of UGT1 levels in rat serum, plasma, and tissue homogenates.

    The UGT1 enzyme, also known as UDP-glucuronosyltransferase 1A1 (UGT1A1) is a key player in the metabolism of drugs and toxins in the liver, making it an essential target for research in the field of metabolism. This kit has a wide detection range of 31.2 pg/mL to 2000 pg/mL with a sensitivity of 7.8 pg/mL, allowing for precise measurement of UGT1 levels in various samples.

    With an assay time of only 1-5 hours and a sample volume of 50-100ul, this kit is efficient and convenient for high-throughput screening. The detection wavelength is 450 nm and the assay principle is quantitative, utilizing the sandwich method for accurate and specific detection of UGT1.

    This ELISA kit is ideal for researchers working in the field of metabolism and drug development. Trust in our reliable and validated Rat UGT1 ELISA Kit to facilitate your research and drive new discoveries.

  • 别名：
    Ugt1a1; Ugt1; UDP-glucuronosyltransferase 1A1; UGT1A1; B1; UDP-glucuronosyltransferase 1-1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.2 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat UGT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 82-91
    1:2 Average % 94
    Range % 89-98
    1:4 Average % 107
    Range % 103-110
    1:8 Average % 110
    Range % 104-114
  • 回收率：
    The recovery of rat UGT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 91-99
    EDTA plasma (n=4) 103 98-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 1.941 2.014 1.978 1.875
    1000 1.503 1.498 1.501 1.398
    500 0.999 1.031 1.015 0.912
    250 0.610 0.631 0.621 0.518
    125 0.417 0.407 0.412 0.309
    62.5 0.235 0.228 0.232 0.129
    31.25 0.148 0.150 0.149 0.046
    0 0.101 0.104 0.103 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol. Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates. Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties. Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II. Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan.
  • 基因功能参考文献：
    1. study indicated that Status Epilepticus altered the expression of brain Ugt1a1 and Ugt1a7, which could alter glucuronidation in the brain. PMID: 29131354
    2. UGT1A1 controls estrogen metabolism. PMID: 28479355
    3. Data indicate that the up-regulation of phase II detoxification in the DR group was confirmed by mRNA and protein expression levels of uridinediphospho-glucuronosyltransferase and glycine-N-acyltransferase in actual liver tissues. PMID: 23230277
    4. Both fipronil and fipronil sulfone treatments induced a 2.5-fold increase in Ugt1a1 and Sult1b1 messenger RNA (mRNA) expressions. PMID: 22447239
    5. UDP-glucuronosyltransferase (UDP-GT) activities towards p-nitrophenol were markedly increased in rats that consumed tea.Induction of UDP-glucuronosyltransferase activity by tea may involve the UDP-GT1 (UGT1A) gene complex of the UDP-GT multigene family PMID: 11983278
    6. These results indicate that the expression of extrahepatic UDP-glucuronosyltransferases UGT1a1 and UGT1a6 is isoform-specific and regulated differentially in tissues by the pituitary gland. PMID: 12153725
    7. Our findings show that the absence of UGT1 protein from Gunn rat hepatocytes is due to rapid degradation of the truncated UGT1 protein by the proteasome and elucidate the molecular basis underlying the deficiency in bilirubin glucuronidation. PMID: 12220528
    8. Developmental stage-specific switching of regulation of the rat UGT1 gene complex was found. PMID: 18938141
    9. Data suggest that thyroid hormones and vitamin A are co-regulators of UDP-glucuronosyltransferase (UGT) 1 expression, without affecting the UGT2 family PMID: 11997190

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    UDP-glycosyltransferase family
  • 数据库链接：