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  4. Rat Substance P,SP ELISA Kit

Rat Substance P,SP ELISA Kit

  • 中文名称：
    大鼠P物质(SP)酶联免疫试剂盒
  • 货号：
    CSB-E08358r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Substance P, SP ELISA Kit is a perfect tool for neuroscience researchers studying Rattus norvegicus. This kit is specifically designed to detect Substance P in a variety of sample types, including serum, plasma, cell culture supernates, and tissue homogenates. With a detection range of 6.25 pg/mL-400 pg/mL and a sensitivity of 1.56 pg/mL, you can be sure of accurate and reliable results.

    The assay principle is quantitative and employs a sandwich measurement technique. The assay time is 1-5h, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, providing precise and dependable readings.

    This Rat Substance P, SP ELISA Kit has been cited in over 6 research publications and is an excellent choice for neuroscience researchers.

  • 别名：
    N/A
  • 缩写：
    SP
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %102
    Range %98-106
    1:4Average %92
    Range %87-95
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of rat SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-97
    EDTA plasma (n=4)9691-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4001.738 1.682 1.710 1.618
    2001.376 1.444 1.410 1.318
    1001.066 1.045 1.056 0.964
    500.672 0.666 0.669 0.577
    250.448 0.434 0.441 0.349
    12.50.302 0.296 0.299 0.207
    6.250.152 0.157 0.155 0.063
    00.093 0.091 0.092 　
  • 数据处理：
  • 货期：
    3-5 working days

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