Human Substance P,SP ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This human substance P (SP) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SP in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of SP bound in the initial step.

Substance P is well-known for its function as a neurotransmitter and a modulator of pain perception by altering cellular signaling pathways. It is released from sensory nerve endings and is widely present in nerve fibers. Substance P is involved in gastrointestinal functioning, memory processing, angiogenesis, vasodilation, and cell proliferation. It also acts on bones and related tissues by binding to receptors, thus regulating bone metabolism, cartilage metabolism, and fracture healing.
别名：

N/A
缩写：

SP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-100
1:2	Average %	102
	Range %	98-105
1:4	Average %	89
	Range %	85-96
1:8	Average %	104
	Range %	100-108

回收率：

The recovery of human SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-92
EDTA plasma (n=4)	85	81-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.