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Rat Prostaglandin E2,PG-E2 ELISA Kit

  • 中文名称：
    大鼠前列腺素E2(PGE2)酶联免疫试剂盒
  • 货号：
    CSB-E07967r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        PGE2（Prostaglandin E2）是一种重要的生理调节因子，它可以影响许多生物过程，包括炎症反应、免疫调节、血管扩张和疼痛传递等。PGE2是一种强烈的炎症介质，其含量水平在许多疾病过程中受到调节，如炎症性肠病、类风湿性关节炎、心血管疾病等。
        j9九游会登录入口首页生物所提供的Rat Prostaglandin E2,PG-E2 ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测大鼠血清、血浆、组织匀浆、细胞裂解液样本中的PG-E2，其灵敏度为0.25 pg/mL，检测范围为0.5 pg/mL-100 pg/mL。
     
  • 缩写：
    PG-E2
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.5 pg/mL-100 pg/mL
  • 灵敏度：
    0.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 91-99  
    1:2 Average % 92  
    Range % 87-96  
    1:4 Average % 93  
    Range % 89-98  
    1:8 Average % 105  
    Range % 99-109  
  • 回收率：
    The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-99  
    EDTA plasma (n=4) 101 96-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    100 0.228 0.224 0.226    
    25 0.365 0.348 0.357    
    8 0.560 0.539 0.550    
    2 0.813 0.787 0.800    
    0.5 1.258 1.234 1.246    
    0 2.031 2.176 2.104    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 客户评论
  • Sample type: Tissue homogenate (Brain)
    Sample species: Mouse
    Review: One model group differed from the control group, the other two did not. The operation steps of the product are still very simple, and the standards are also prepared with different concentrations.

靶点详情

  • 最新研究进展：
    PGE2是一种前列腺素，与炎症和癌症等多种疾病有关。近期的研究表明，PGE2可以在体内诱导肝癌干细胞分化为肝癌细胞，从而促进肝癌的发生和发展。此外，研究人员还发现，通过靶向PGE2的合成酶和受体可以减少肿瘤的生长和转移，从而为肿瘤治疗提供新的策略。