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  4. Human Prostaglandin E2,PG-E2 ELISA Kit

Human Prostaglandin E2,PG-E2 ELISA Kit

  • 中文名称：
    人前列腺素E2(PGE2)酶联免疫试剂盒
  • 货号：
    CSB-E07965h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PG-E2 ELISA Kit was designed for the quantitative measurement of Human PG-E2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.5 pg/mL-300 pg/mL and the sensitivity is 0.75 pg/mL.

  • 缩写：
    PG-E2
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.5 pg/mL-300 pg/mL
  • 灵敏度：
    0.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-99  
    1:2 Average % 92  
    Range % 86-96  
    1:4 Average % 97  
    Range % 92-103  
    1:8 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of human PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 90 83-94  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    300 0.193 0.201 0.197    
    75 0.349 0.327 0.338    
    24 0.523 0.531 0.527    
    6 0.903 0.962 0.933    
    1.5 1.316 1.393 1.355    
    0 2.271 2.212 2.242    
  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

Can this kit test cell supernatnat?

A:
Thanks for your inquiry. Usually we suggest you test the sample types mentioned in the kit manual. If you want to test cell supernatnat samples, you can do a pretest first.