Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Plasminogen (Plg) ELISA kit

Rat Plasminogen (Plg) ELISA kit

  • 中文名称：
    大鼠纤溶酶原(Plg)酶联免疫试剂盒
  • 货号：
    CSB-E17250r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        Plg（Plasminogen）是一种由肝脏合成并广泛存在于血液中的单链糖蛋白，是体内溶酶原的前体，可被活化成为溶解纤维蛋白和其他基质蛋白，参与体内纤维蛋白和其他蛋白质的降解和清除。Plg水平的升高与炎症、血栓形成、肿瘤和其他疾病的发生相关。
        j9九游会登录入口首页生物所提供的Rat Plasminogen (Plg) ELISA kit属于ELISA检测试剂盒，采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的Plg，其灵敏度为15.6 ng/mL，检测范围为62.5 ng/mL-4000 ng/mL。
     
  • 别名：
    Plg ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 ng/mL-4000 ng/mL
  • 灵敏度：
    15.6 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Plg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 96    
    Range % 86-103    
    1:200 Average % 91    
    Range % 83-99    
    1:400 Average % 95    
    Range % 82-97    
    1:800 Average % 95    
    Range % 93-100    
  • 回收率：
    The recovery of rat Plg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 91-102    
    EDTA plasma (n=4) 94 85-99    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    4000 0.121 0.116 0.119      
    2000 0.223 0.230 0.227      
    1000 0.468 0.475 0.472      
    500 0.774 0.785 0.78      
    250 1.225 1.213 1.219      
    125 1.689 1.674 1.682      
    62.5 2.162 2.179 2.171      
    0 2.608 2.594 2.601      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    Plg是一种广泛存在于人体内的血浆蛋白，在多种疾病的发生和发展中可能发挥着重要的作用。最近的研究表明，Plg参与了肿瘤生长、血管生成、转移和免疫逃逸等多个方面的调节，并在心血管疾病、肺部疾病和肝脏疾病中的作用备受关注。对Plg结构和功能的研究也取得了重要进展，这将有助于更深入地了解Plg的生物学特性，为新药物的开发和治疗策略的制定提供更可靠的理论基础。
  • 功能：
    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.; Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • 基因功能参考文献：
    1. Angiostatin overexpression was associated with attenuated renal disease progression in a model of chronic kidney injury, likely because of its anti-inflammatory actions. PMID: 18971211
    2. Expression of angiostatin in the ischemic tissues after ischemia-reperfusion in diabetes mellitus + cerebral ischemia group increased significantly. PMID: 19932587
    3. plasmin has a role as an intracellular protease PMID: 12928439
    4. Plasminogen-induced IL-1beta, TNF-alpha, and iNOS mRNA expression in primary cultured rat microglia and BV2 murine microglial cells. PMID: 14651966
    5. Plg gene expression is up-regulated by nerve growth factor PMID: 17663741
    6. Plasmin activates epithelial Na+ channels by cleaving the gamma subunit PMID: 18981180

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasminogen subfamily
  • 数据库链接：