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  4. Rat Neuroglobin,NGB ELISA Kit

Rat Neuroglobin,NGB ELISA Kit

  • 中文名称：
    大鼠脑红蛋白(NGB)酶联免疫试剂盒
  • 货号：
    CSB-E08738r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat NGB ELISA Kit was designed for the quantitative measurement of Rat NGB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

  • 别名：
    Ngb ELISA Kit; Neuroglobin ELISA Kit
  • 缩写：
    NGB
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NGB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %99
    Range %96-102
    1:4Average %93
    Range %90-96
    1:8Average %88
    Range %84-92
  • 回收率：
    The recovery of rat NGB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 104100-108
    EDTA plasma (n=4)9894-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.256 2.104 2.180 2.035
    5001.678 1.637 1.658 1.513
    2501.131 1.168 1.150 1.005
    1250.742 0.765 0.754 0.609
    62.50.510 0.500 0.505 0.360
    31.20.416 0.401 0.409 0.264
    15.60.301 0.311 0.306 0.161
    00.144 0.146 0.145
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes.
  • 基因功能参考文献：
    1. Cerebral hemorrhage significantly inhibited the spatial learning and memory ability of rats; the mechanism may be related to decreased cerebral expression of BDNF and NGB PMID: 28829495
    2. In conclusion, this study shows that EPOR and Ngb were upregulated, and both of them act as important coordinated neuroprotectors in rh-EPO treatment of PWMD. However, the two proteins exhibit different expression patterns. PMID: 28359933
    3. Ngb expression is limited to the subpopulation of type I spiral ganglion cells, those which innervate inner hair cells, while the subpopulation of type II spiral ganglion cells which innervate the outer hair cells do not express Ngb. The cochlear nuclei and superior olivary complex (SOC) also express considerable amounts of Ngb. PMID: 25636685
    4. The present study first reported the yeast expressed production of Tat-NGB-His and His-NGB-His, and then elucidated the transduction and neuroprotection of Tat-NGB-His on PC12 cell PMID: 26646387
    5. Intermittent hypobaric hypoxia preconditioning activated the expression of neuroglobin and Bcl-2 in the rat hippocampal CA1 area following ischemia-reperfusion, and prevented hippocampal neuronal apoptosis. PMID: 26400308
    6. NEUROGLOBIN DISTRIBUTION IN THE RAT CEREBELLAR PURKINJE CELLS PMID: 26983282
    7. 1-Bromopropane(1-BP)resulted in decreased endogenous neuroprotectant neuroglobin in cerebral cortex, which might play an important role in central nervous system neurotoxicity induced by 1-BP. PMID: 25557405
    8. Study demonstrated that LED light exposure up-regulated NGB expression in rat retinas; alteration was induced predominantly by blue light compared to green or red light, and increased NGB levels were correlated with the severity of retinal light damage PMID: 24747803
    9. antioxidants in severe traumatic brain injury do not seem to support the role of neuroglobin as an endogenous neuroprotective antioxidant agent PMID: 24491879
    10. These findings indicated that Ngb might play an important neuro-protective effect after subarachnoid hemorrhage. PMID: 24281943
    11. It induces neuronal amyloid beta production. PMID: 23673310
    12. involved in pathogenesis of sepsis-associated encephalopathy and has neuroprotective effects PMID: 24145836
    13. High Neuroglobin levels are associated with reactive gliosis in response to eye injury. PMID: 23639750
    14. The results of this study suggested that Ngb could be involved in sleep regulation, independent of its role in the control of oxidative stress. PMID: 23262504
    15. Up-regulation of Ngb expression played an important role in the neuroprotection induced by limb ischemic preconditioning. PMID: 23180278
    16. Ngb mRNA expression in brain is associated with oxidative stress caused by arsenite exposure in rats. PMID: 22887765
    17. Neuroglobin could protect neurons from traumatic stress and secondary ischemia and hypoxia insults during ultra-early and acute stages of acute brain injury. PMID: 20476562
    18. NGB was upregulated in traumatic brain injury PMID: 22664218
    19. we showed that the NGB expression level in neurons increased continuously from 2 h after injury, and reached a peak at 16 h after which it decreased sharply PMID: 21915648
    20. We found that NGB was present in a rat astrocytoma cell line (C6), human astrocytoma cell line (U251), and human astrocytoma tissues PMID: 22009023
    21. Data support the hypothesis that an up-regulation of neuroglobin could be a compensatory mechanism in response to the hypoxic-ischemic insults caused by seizures in pups during intrauterine life. PMID: 21767627
    22. Ngb expression quickly increases when retinal ganglion cells die in acute retina ischemia, and expression is mainly intracytoplasmic. PMID: 21054965
    23. Cygb but not Ngb may protect cerebellar cells from the chronic presence of carbon monoxide exposure during prenatal and postnatal development. PMID: 20230802
    24. Neuroglobin expression was decreasd in the cochlea of rat pups exposed to chronic very mild carbon monoxide in air during and after the prenatal period. PMID: 20211612
    25. results indicate that the Ngb could be involved in both photic and nonphotic entrainment via input from the geniticulo-hypothalamic tract PMID: 20187147
    26. Up-regulation of neuroglobin expression in brain injury induced by LPS is correlated with duration after challenge of LPS. PMID: 19842562
    27. NGB mRNA and protein are abundantly expressed and widely distributed throughout the adult rat brain, as seen in the NGB-immunopositive signals of neuronal cell bodies and processes. PMID: 11820779
    28. acts as an endogenous neuroprotective factor in focal cerebral ischemia PMID: 12621155
    29. Examination of the cell type-specific expression of neuroglobin in neurons and astroglial cells in primary cultures of rat fetal hippocampal cells finds neuroglobin is only expressed in neurons. PMID: 15193759
    30. is present in the carotid body and increases after chronic intermittent hypoxia PMID: 16683692
    31. hypoxic stimulus presentation is a major determinant of the regulation of hypoxic sensitive genes such as Ngb PMID: 16750520
    32. it is unlikely that neuroglobin is instrumental in the acute response of neurons to hypoxic or ischemic insults PMID: 16796995
    33. Therefore, Ngb may act as an intracellular ROS scavenger, and such antioxidant properties may play a protective role against Abeta-induced cell injury. PMID: 17560688
    34. Recombinant neuroglobin is fused to human HIV-1 transactivator-of-transcription protein-transduction domain (TAT[49-57] PTD), enters primary cultured cortical neurons in a dose-dependent manner and protects the neurons from hypoxia-induced apoptosis. PMID: 17576199
    35. A diurnal variation of Ngb mRNA in the suprachiasmatic nucleus, having a peak in the day time (light-period) and nadir during night (dark-period). PMID: 18451642
    36. The cellular and physiological function of Ngb in different subsets of neurones might not be identical. PMID: 18509243
    37. Results demonstrate by immunohistochemical analysis the colocalization of neuroglobin and PrP(c) in the retinal ganglion cell layer. PMID: 19327369
    38. the role of Ngb during aging and hypoxia PMID: 19536481

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  • 亚细胞定位：
    Perikaryon.
  • 蛋白家族：
    Globin family
  • 组织特异性：
    Widely distributed throughout the adult brain, including cerebral cortex, hippocampus, thalamus, hypothalamus, olfactory bulb, and cerebellum.
  • 数据库链接：