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  4. Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit

Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit

  • 中文名称：
    大鼠巨噬细胞移动抑制因子(MIF)酶联免疫试剂盒
  • 货号：
    CSB-E07293r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 别名：
    Mif ELISA Kit; Macrophage migration inhibitory factor ELISA Kit; MIF ELISA Kit; EC 5.3.2.1 ELISA Kit; Glutathione-binding 13 kDa protein ELISA Kit; L-dopachrome isomerase ELISA Kit; L-dopachrome tautomerase ELISA Kit; EC 5.3.3.12 ELISA Kit; Phenylpyruvate tautomerase ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 95  
    Range % 90-100  
    1:10 Average % 98  
    Range % 92-102  
    1:20 Average % 94  
    Range % 89-99  
    1:40 Average % 92  
    Range % 82-96  
  • 回收率：
    The recovery of rat MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-94  
    EDTA plasma (n=4) 96 92-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.712 2.611 2.662 2.507  
    2000 2.054 1.953 2.004 1.849  
    1000 1.286 1.307 1.297 1.142  
    500 0.748 0.737 0.743 0.588  
    250 0.478 0.467 0.473 0.318  
    125 0.360 0.371 0.366 0.211  
    62.5 0.279 0.275 0.277 0.122  
    0 0.155 0.154 0.155 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 亚细胞定位：
    Secreted. Cytoplasm.
  • 蛋白家族：
    MIF family
  • 组织特异性：
    Expressed in a wide variety of organs including brain, spleen, liver, muscle and kidney.
  • 数据库链接：