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  4. Rat Interleukin 15,IL-15 ELISA KIT

Rat Interleukin 15,IL-15 ELISA KIT

  • 中文名称：
    大鼠白介素15(IL-15)酶联免疫试剂盒
  • 货号：
    CSB-E07453r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat IL15 ELISA Kit was designed for the quantitative measurement of Rat IL15 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

  • 别名：
    Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %90-101
    1:2Average %93
    Range %87-99
    1:4Average %87
    Range %82-94
    1:8Average %100
    Range %95-104
  • 回收率：
    The recovery of rat IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-101
    EDTA plasma (n=4)9387-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.511 2.567 2.539 2.383
    501.773 1.783 1.778 1.622
    251.106 1.154 1.130 0.974
    12.50.704 0.725 0.715 0.559
    6.250.415 0.431 0.423 0.267
    3.120.287 0.297 0.292 0.136
    1.560.214 0.231 0.223 0.067
    00.155 0.157 0.156 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation.
  • 基因功能参考文献：
    1. study indicates that training, skeletal muscle phenotype, and metabolic status all influence the temporal pattern of post-exercise changes in IL-15 expression. PMID: 25522723
    2. found that in diabetic rats, resistance training increased muscle and serum IL-15 levels PMID: 24006180
    3. results suggested that IL-15-Akt signaling harbored the complex signals for muscle-T cell interaction, the regulatory networks have significant potential for the restriction on IL-1beta inflammatory signaling PMID: 23017227
    4. IL-15 is related to organ dysfunction during severe acute pancreatitis, and it functions as a protective factor against the organ injuries. PMID: 20332642
    5. The results of this study suggest that the IL-15 signal transduction pathway in neural cells is similar to that in immune cells. PMID: 19747902
    6. IL-15 might antagonize some cytokine mediated cell death in islet cells. PMID: 12880684
    7. IL-15 produced by metastatic colon carcinoma cells can induce hyperplasia in the mucosa adjacent to colon cancer, thus contributing to angiogenesis and progression of the disease PMID: 14581351
    8. Cocultivation of lymphocytes with pancreatic stellate cells and IL-15 inhibited spontaneous lymphocyte apoptosis, and this effect was reduced by an anti-IL-15 antibody PMID: 16002563
    9. the decrease of CORT secretion by IL-15 is in part because of (i) the decrease of adenylate cyclase activity and cAMP production and (ii) the inhibition of 3beta-hydroxysteroid dehydrogenase and 11beta-hydroxylase activities in rat ZFR cells. PMID: 16405651
    10. Increases in IL-15 mRNA in response to atrophic stimuli may be an attempt to counteract muscle mass loss in skeletal muscles of old animals. PMID: 17135303
    11. IL-15 might have an inhibitory effect on the physiologic vascular remodeling processes in closing the ductus arteriosus. PMID: 17667861
    12. The changes observed in BAT suggest that IL-15 could be implicated in lipid consumption in this tissue by regulating lipid oxidation and probably thermogenesis, processes mediated by UCP1 and UCP3 and PPARdelta and PPARalpha. PMID: 18239634
    13. Preservation of IL-15 signaling by calorie restriction may therefore represent a further mechanism contributing to the anti-aging effect of this dietary intervention in skeletal muscle PMID: 19396981

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-15/IL-21 family
  • 数据库链接：

    KEGG: rno:25670

    UniGene: Rn.2490