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  4. Rat Arachidonate 5-lipoxygenase (Alox5) ELISA kit

Rat Arachidonate 5-lipoxygenase (Alox5) ELISA kit

  • 中文名称：
    大鼠花生四烯酸5脂氧合酶(Alox5)酶联免疫试剂盒
  • 货号：
    CSB-E16982r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Alox5 ELISA Kit was designed for the quantitative measurement of Rat Alox5 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Alox5Arachidonate 5-lipoxygenase ELISA Kit; 5-LO ELISA Kit; 5-lipoxygenase ELISA Kit; EC 1.13.11.34 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Alox5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 96    
    Range % 92-100    
    1:2 Average % 102    
    Range % 95-106    
    1:4 Average % 90    
    Range % 83-94    
    1:8 Average % 93    
    Range % 88-97    
  • 回收率：
    The recovery of rat Alox5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 84-95    
    EDTA plasma (n=4) 100 92-105    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    10 2.817 2.743 2.780 2.666    
    5 2.369 2.304 2.337 2.223    
    2.5 1.574 1.521 1.548 1.434    
    1.25 0.825 0.789 0.807 0.693    
    0.625 0.418 0.402 0.410 0.296    
    0.312 0.234 0.228 0.231 0.117    
    0.156 0.161 0.165 0.163 0.049    
    0 0.113 0.115 0.114 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the oxygenation of arachidonate to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. Also may play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells.
  • 基因功能参考文献：
    1. 5-LOX plays an important role in activating apoptosis and repressing neutrophils activation during acute necrotizing pancreatitis. PMID: 29421656
    2. Our results indicate that 5-lipoxygenase activation is mechanistically involved in chronic hepatic injury in a rat model of chronic aluminum overload exposure and that the 5-LO signaling pathway, which associated with inflammation and oxidative stress, is a potential therapeutic target for chronic non-infection liver diseases PMID: 27368151
    3. Results support 5-lipoxygenase involvement in the effect of glutoxim and molixan on intracellular Ca(2+) concentration in macrophages and indicate the inadvisability of a combined use of drugs glutoxim and molixan and antiasthmatic agent zileuton PMID: 27599517
    4. 12/15-LO-derived oxidized lipids regulate histone modifications associated with profibrotic gene expression in MCs, and 12/15-LO can mediate similar actions of TGF-beta1 and diabetes. PMID: 26492974
    5. Alox5 affects the osteogenic and adipogenic abilities of iPSCs in vivo and the effect of Cox2 inhibition in this system. PMID: 24906289
    6. 5-Lipoxygenase and 5-LO activating protein (FLAP) colocalize in the interstitium of the renal medulla following trauma and hemorrhagic shock.. PMID: 24747451
    7. Urotensin II increased aortic adventitial fibroblast expression of the 5-LO mRNA/protein by NF-kappaB activation through p38 MAPK and ERK pathways. PMID: 23053343
    8. 5-lipoxygenase expression can be considered as a critical event in controlling the process of mammary tumor development. PMID: 22213124
    9. Cochinchina momordica seed extracts exerted significantly protective effect against cysteamine-induced duodenal ulcer by 5-lipoxygenase inhibition. PMID: 22486866
    10. Zileuton can reduce rotenone-induced 5-LOX activation and cell injury in PC12 cells. PMID: 21488210
    11. Cochinchina momordica seed extract is a gastroprotective agent against acute gastric mucosal damage by suppressing proinflammatory cytokines, downregulating cPLA(2), 5-LOX, and increasing the synthesis of mucus. PMID: 19130224
    12. Our results confirm the key role of both COX-2 and 5-LOX in the inflammatory response to acute pancreatitis. PMID: 20977452
    13. ALOX5 gene silencing decreases the frequency of necrotic cell death after anoxia-reoxygenation of cultured cardiomyocytes as well as reduces heart infarct area size at ischemia-reperfusion in vivo. PMID: 20011686
    14. prolonged LPS treatment modifies activity in alveolar macrophages PMID: 12385890
    15. hyperoxia increases LTB(4) output, as well as protein levels of 5-LO and FLAP, in newborn rat lungs during early postnatal life. PMID: 12490039
    16. Enzyme is down-regulated and leukotriene production reduced by prolonged exposure to lipopolysaccharide. PMID: 12664571
    17. Role of 5-Lipoxygenase in angiotensin II-induced NAD(P)H-oxidase activation. PMID: 12914802
    18. Plays critical role in progression of pulmonary hypertension. Detrimental effect of 5-lipoxygenase is manifest only in setting of pulmonary vascular endothelial cell dysfunction. PMID: 14726295
    19. arachidonic acid metabolism through LOX activity, is a major source of extracellular O(2)(*-) release in skeletal muscle. PMID: 15107407
    20. iNOS regulates alveolar macrophage 5-lipoxygenase metabolism following endotoxin exposure. PMID: 15371096
    21. 5-lipoxygenase nuclear import is regulated by multiple nuclear localization sequences PMID: 15479450
    22. Basal iPLA2 activity, through the generation of 5-LO metabolites, regulates AMPA receptor phosphorylation of GluR1 subunits, an effect that might selectively influence the number of membrane receptors in area CA1 of the hippocampus. PMID: 15630695
    23. Activation of the 5-lipoxygenase pathway previously reported in streptozotocin-induced diabetic rats could be explained by an augmented sensitivity to cysteinyl leukotrienes of the diabetic aorta. PMID: 15789618
    24. Our results suggest that Nordy was capable of reducing the level of malignancy of glioma cells. PMID: 16516855
    25. From these results, we conclude that OGD can activate 5-LOX in primary rat cortical neurons, and that this activation may be partly mediated via activating NMDA receptor. PMID: 16606359
    26. Data show that the rat air pouch model is a reliable and useful tool for evaluating in vivo efficacy of 5-LOX inhibitors. PMID: 18295198
    27. atorvastatin and pioglitazone induced an interaction between 5LO and COX2 in the cytosolic fraction of myocardial cells PMID: 18714024
    28. nuclear import, which is regulated by phosphorylation on Ser-523, to determine the subcellular distribution of 5-LO, which in turn regulates leukotriene biosynthesis. PMID: 18978352
    29. Lysophosphatidylcholine caused the enhancement of oxidative stress in aged aorta through reactive species generation by an activated 5-lipoxygenase pathway. PMID: 19226207

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  • 亚细胞定位：
    Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Cytoplasm, perinuclear region. Cytoplasm, cytosol. Nucleus envelope. Nucleus intermembrane space.
  • 蛋白家族：
    Lipoxygenase family
  • 数据库链接：

    KEGG: rno:25290

    STRING: 10116.ENSRNOP00000017633

    UniGene: Rn.9662