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  4. Recombinant Rat Arachidonate 5-lipoxygenase (Alox5)

Recombinant Rat Arachidonate 5-lipoxygenase (Alox5)

  • 中文名称：
    Recombinant Rat Arachidonate 5-lipoxygenase(Alox5),Yeast
  • 货号：
    CSB-YP001624RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Arachidonate 5-lipoxygenase(Alox5),Yeast
  • 货号：
    CSB-EP001624RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Arachidonate 5-lipoxygenase(Alox5),Yeast
  • 货号：
    CSB-EP001624RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Arachidonate 5-lipoxygenase(Alox5),Yeast
  • 货号：
    CSB-BP001624RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Arachidonate 5-lipoxygenase(Alox5),Yeast
  • 货号：
    CSB-MP001624RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Alox5Arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase; EC 1.13.11.34
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-673
  • 氨基酸序列
    MPSYTVTVAT GSQWFAGTDD YIYLSLIGSA GCSEKHLLDK AFYNDFERGG RDSYDVTVDE ELGEIYLVKI EKRKYRLHDD WYLKYITLKT PHDYIEFPCY RWITGEGEIV LRDGCAKLAR DDQIHILKQH RRKELETRQK QYRWMEWNPG FPLSIDAKCH KDLPRDIQFD SEKGVDFVLN YSKAMENLFI NRFMHMFQSS WHDFADFEKI FVKISNTISE RVKNHWQEDL MFGYQFLNGC NPVLIKRCTE LPKKLPVTTE MVECSLERQL SLEQEVQEGN IFIVDYELLD GIDANKTDPC THQFLAAPIC LLYKNLANKI VPIAIQLNQT PGEKNPIFLP TDSKYDWLLA KIWVRSSDFH IHQTITHLLR THLVSEVFGI AMYRQLPAVH PLFKLLVAHV RFTIAINTKA REQLNCEYGL FDKANATGGG GHVQMVQRAV QDLTYSSLCF PEAIKARGMD NTEDIPYYFY RDDGLLVWEA IQSFTTEVVS IYYEDDQVVE EDQELQDFVK DVYVYGMRGR KASGFPKSIK SREKLSEYLT VVIFTASAQH AAVNFGQYDW CSWIPNAPPT MRAPPPTAKG VVTIEQIVDT LPDRGRSCWH LGAVWALSQF QENELFLGMY PEEHFIEKPV KEAMIRFRKN LEAIVSVIAE RNKNKKLPYY YLSPDRIPNS VAI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the oxygenation of arachidonate to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. Also may play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells.
  • 基因功能参考文献：
    1. 5-LOX plays an important role in activating apoptosis and repressing neutrophils activation during acute necrotizing pancreatitis. PMID: 29421656
    2. Our results indicate that 5-lipoxygenase activation is mechanistically involved in chronic hepatic injury in a rat model of chronic aluminum overload exposure and that the 5-LO signaling pathway, which associated with inflammation and oxidative stress, is a potential therapeutic target for chronic non-infection liver diseases PMID: 27368151
    3. Results support 5-lipoxygenase involvement in the effect of glutoxim and molixan on intracellular Ca(2+) concentration in macrophages and indicate the inadvisability of a combined use of drugs glutoxim and molixan and antiasthmatic agent zileuton PMID: 27599517
    4. 12/15-LO-derived oxidized lipids regulate histone modifications associated with profibrotic gene expression in MCs, and 12/15-LO can mediate similar actions of TGF-beta1 and diabetes. PMID: 26492974
    5. Alox5 affects the osteogenic and adipogenic abilities of iPSCs in vivo and the effect of Cox2 inhibition in this system. PMID: 24906289
    6. 5-Lipoxygenase and 5-LO activating protein (FLAP) colocalize in the interstitium of the renal medulla following trauma and hemorrhagic shock.. PMID: 24747451
    7. Urotensin II increased aortic adventitial fibroblast expression of the 5-LO mRNA/protein by NF-kappaB activation through p38 MAPK and ERK pathways. PMID: 23053343
    8. 5-lipoxygenase expression can be considered as a critical event in controlling the process of mammary tumor development. PMID: 22213124
    9. Cochinchina momordica seed extracts exerted significantly protective effect against cysteamine-induced duodenal ulcer by 5-lipoxygenase inhibition. PMID: 22486866
    10. Zileuton can reduce rotenone-induced 5-LOX activation and cell injury in PC12 cells. PMID: 21488210
    11. Cochinchina momordica seed extract is a gastroprotective agent against acute gastric mucosal damage by suppressing proinflammatory cytokines, downregulating cPLA(2), 5-LOX, and increasing the synthesis of mucus. PMID: 19130224
    12. Our results confirm the key role of both COX-2 and 5-LOX in the inflammatory response to acute pancreatitis. PMID: 20977452
    13. ALOX5 gene silencing decreases the frequency of necrotic cell death after anoxia-reoxygenation of cultured cardiomyocytes as well as reduces heart infarct area size at ischemia-reperfusion in vivo. PMID: 20011686
    14. prolonged LPS treatment modifies activity in alveolar macrophages PMID: 12385890
    15. hyperoxia increases LTB(4) output, as well as protein levels of 5-LO and FLAP, in newborn rat lungs during early postnatal life. PMID: 12490039
    16. Enzyme is down-regulated and leukotriene production reduced by prolonged exposure to lipopolysaccharide. PMID: 12664571
    17. Role of 5-Lipoxygenase in angiotensin II-induced NAD(P)H-oxidase activation. PMID: 12914802
    18. Plays critical role in progression of pulmonary hypertension. Detrimental effect of 5-lipoxygenase is manifest only in setting of pulmonary vascular endothelial cell dysfunction. PMID: 14726295
    19. arachidonic acid metabolism through LOX activity, is a major source of extracellular O(2)(*-) release in skeletal muscle. PMID: 15107407
    20. iNOS regulates alveolar macrophage 5-lipoxygenase metabolism following endotoxin exposure. PMID: 15371096
    21. 5-lipoxygenase nuclear import is regulated by multiple nuclear localization sequences PMID: 15479450
    22. Basal iPLA2 activity, through the generation of 5-LO metabolites, regulates AMPA receptor phosphorylation of GluR1 subunits, an effect that might selectively influence the number of membrane receptors in area CA1 of the hippocampus. PMID: 15630695
    23. Activation of the 5-lipoxygenase pathway previously reported in streptozotocin-induced diabetic rats could be explained by an augmented sensitivity to cysteinyl leukotrienes of the diabetic aorta. PMID: 15789618
    24. Our results suggest that Nordy was capable of reducing the level of malignancy of glioma cells. PMID: 16516855
    25. From these results, we conclude that OGD can activate 5-LOX in primary rat cortical neurons, and that this activation may be partly mediated via activating NMDA receptor. PMID: 16606359
    26. Data show that the rat air pouch model is a reliable and useful tool for evaluating in vivo efficacy of 5-LOX inhibitors. PMID: 18295198
    27. atorvastatin and pioglitazone induced an interaction between 5LO and COX2 in the cytosolic fraction of myocardial cells PMID: 18714024
    28. nuclear import, which is regulated by phosphorylation on Ser-523, to determine the subcellular distribution of 5-LO, which in turn regulates leukotriene biosynthesis. PMID: 18978352
    29. Lysophosphatidylcholine caused the enhancement of oxidative stress in aged aorta through reactive species generation by an activated 5-lipoxygenase pathway. PMID: 19226207

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  • 亚细胞定位：
    Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Cytoplasm, perinuclear region. Cytoplasm, cytosol. Nucleus envelope. Nucleus intermembrane space.
  • 蛋白家族：
    Lipoxygenase family
  • 数据库链接：

    KEGG: rno:25290

    STRING: 10116.ENSRNOP00000017633

    UniGene: Rn.9662