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  4. Pig interleukin 4,IL-4 ELISA Kit

Pig interleukin 4,IL-4 ELISA Kit

  • 中文名称：
    猪白介素4(IL-4)酶联免疫试剂盒
  • 货号：
    CSB-E06785p
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig IL4 ELISA Kit was designed for the quantitative measurement of Pig IL4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

  • 别名：
    IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %82-91
    1:2Average %90
    Range %85-95
    1:4Average %89
    Range %84-93
    1:8Average %96
    Range %92-101
  • 回收率：
    The recovery of pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-105
    EDTA plasma (n=4)9286-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.719 2.652 2.686 2.492
    2001.962 1.861 1.912 1.718
    1001.213 1.204 1.209 1.015
    500.748 0.689 0.719 0.525
    250.551 0.527 0.539 0.345
    12.50.355 0.374 0.365 0.171
    6.250.295 0.272 0.284 0.090
    00.192 0.196 0.194 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 基因功能参考文献：
    1. IL-4- and IL-10-producing invariant NKT cells inhibit the Th1 cell response, but not the Th17 cell response PMID: 21572032
    2. IL-4 induced activation of Akt/SREBP-1/lipid biosynthesis in EC, resulting in protection against membrane attack complex and melittin, in association with mitochondrial protection. PMID: 20017192
    3. There was no significant difference of IL-4 production between fresh and frozen peripheral blood mononuclear cells. PMID: 19766617
    4. The endothelium and subendothelium of porcine iliac arteries that are transduced with recombinant pig IL-4 are effectively protected from complement-dependent immediate injury after perfusion with human blood. PMID: 17082655
    5. unlike in humans and mice, porcine IL-4 blocks antibody and IL-6 secretion and suppresses antigen-stimulated proliferation of B cells PMID: 18761033

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 数据库链接：