Pig Nuclear factor-kappa B,NF-κB ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本试剂盒（CSB-E13448p）采用双抗体夹心酶联免疫法，专为定量检测猪血清、血浆、组织匀浆及细胞裂解液样本中的核因子-κB（NF-κB）设计。NF-κB作为重要的转录调控因子，在炎症反应、免疫应答和细胞增殖等生物学过程中发挥核心作用，其动态监测对研究猪类疾病机制、免疫调控及药物干预具有重要意义。试剂盒检测灵敏度为31.25 pg/mL，线性范围覆盖31.25-2000 pg/mL，通过预包被特异性捕获抗体与标记检测抗体的协同作用实现精准定量。该产品适用于动物模型构建、病原体感染机制研究、疫苗效力评估等科研领域，可兼容离心后澄清的液态样本及适当稀释的细胞裂解物，实验过程包含标准品梯度稀释、样本孵育、显色反应及酶标仪检测等标准化流程，为科研工作者提供稳定可靠的NF-κB活性动态分析方案。
别名：

NF-kappa B,Nuclear factor-kappa B
缩写：

NF-κB
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-95
1:2	Average %	103
	Range %	99-106
1:4	Average %	93
	Range %	87-98
1:8	Average %	91
	Range %	88-95

回收率：

The recovery of pig NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	90	86-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.