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Pig C-reactive protein,CRP ELISA Kit

  • 中文名称：
    猪C反应蛋白(CRP)酶联免疫试剂盒
  • 货号：
    CSB-E08163p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig CRP ELISA Kit was designed for the quantitative measurement of Pig CRP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    CRP ELISA kit; PTX1C-reactive protein ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 93-100  
    1:2 Average % 104  
    Range % 101-108  
    1:4 Average % 85  
    Range % 81-89  
    1:8 Average % 93  
    Range % 90-96  
  • 回收率：
    The recovery of pig CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 82-91  
    EDTA plasma (n=4) 84 81-89  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    4000 2.079 2.098 2.089 1.951  
    2000 1.598 1.577 1.588 1.450  
    1000 0.934 0.998 0.966 0.828  
    500 0.557 0.567 0.562 0.424  
    250 0.386 0.387 0.387 0.249  
    125 0.283 0.276 0.280 0.142  
    62.5 0.201 0.203 0.202 0.064  
    0 0.137 0.139 0.138    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • 基因功能参考文献：
    1. Enhanced external counterpulsation decreased C-reactive protein and increased flow-mediated dilation in hypercholesterolemia. PMID: 22681602
    2. serum C-reactive protein correlates with macrophage accumulation and coronary artery disease in hypercholesterolemic pigs PMID: 12754176
    3. CRP inhibits endothelium-dependent NO-mediated dilation in coronary arterioles by producing superoxide from NAD(P)H oxidase via p38 kinase activation. PMID: 15718491
    4. The present study shows that elevated levels of serum CRP and IL-10 were associated with porcine circovirus type 2-infected piglets that subsequently developed severe PMWS. (postweaning multisystemic wasting syndrome) PMID: 16817761
    5. These data suggest that CRP might play a pathogenic role in the development of myointimal hyperplasia in polytetrafluoroethylene grafts. PMID: 17510097
    6. myocardial infarction induced proinflammatory gene and protein expression in peripheral blood mononuclear cells of tissue factor cyclo-oxygenase-2, monocyte chemoattractant protein-1 and CRP PMID: 19036073
    7. CRP stimulates superoxide production and the subsequent formation of peroxynitrite from basal released nitric oxide compromises PGI(2) synthesis. PMID: 19410579
    8. Data show that during posttranplant obliterative bronchiolitis development, CRP is localized in inflammatory cells, myofibroblasts and endothelial cells probably as a part of the local inflammatory response. PMID: 19503785

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Pentraxin family
  • 组织特异性：
    Found in plasma.
  • 数据库链接：