Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse galanin prepropeptide (GAL) ELISA kit

Mouse galanin prepropeptide (GAL) ELISA kit

  • 中文名称：
    小鼠甘丙肽前肽前体(GAL)酶联免疫试剂盒
  • 货号：
    CSB-EL009191MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse GAL ELISA Kit was designed for the quantitative measurement of Mouse GAL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    Gal ELISA Kit; Galn ELISA Kit; Galanin peptides [Cleaved into: Galanin; Galanin message-associated peptide ELISA Kit; GMAP)] ELISA Kit
  • 缩写：
    GAL
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 96
    Range % 92-104
    1:2 Average % 93
    Range % 86-98
    1:4 Average % 92
    Range % 87-95
    1:8 Average % 102
    Range % 96-107
  • 回收率：
    The recovery of mouse GAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 88-95
    EDTA plasma (n=4) 107 101-112
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.621 2.714 2.668 2.558
    1000 2.203 2.320 2.262 2.152
    500 1.655 1.699 1.677 1.567
    250 1.047 1.122 1.085 0.975
    125 0.788 0.745 0.767 0.657
    62.5 0.463 0.462 0.463 0.353
    31.25 0.212 0.223 0.218 0.108
    0 0.108 0.111 0.110 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Endocrine hormone of the central and peripheral nervous systems that binds and activates the G protein-coupled receptors GALR1, GALR2, and GALR3. This small neuropeptide may regulate diverse physiologic functions including contraction of smooth muscle of the gastrointestinal and genitourinary tract, growth hormone and insulin release and adrenal secretion.
  • 基因功能参考文献：
    1. Galanin, acting via the GAL1 receptor and Gi -coupled signalling in L and K cells, is a potent inhibitor of GLP-1 and GIP secretion PMID: 26661062
    2. Galanin released by cancer induces neuritogenesis, facilitating perineural invasion. PMID: 25917569
    3. Study shows that LIF-treated postmitotic oligodendrocytes secrete a number of neuropeptides, in particular galanin, at the mRNA and peptide level, and galanin itself was shown to be a survival factor for oligodendrocytes PMID: 25639936
    4. Results suggest that GAL is a regulator of myelination, as demonstrated by MBP synthesis, and may be one of the myelination promoters PMID: 25450959
    5. Oleuropein protects mice from gh-fat diet induced adiposity by upregulation of genes involved in WNT10b-mediated signaling and downregulation of genes involved in galanin-mediated signaling cascades. PMID: 25104077
    6. medial preoptic area galanin neurons emerge as an essential regulatory node of male and female parenting behaviour and other social responses PMID: 24828191
    7. Analysis of the gene proximal region using EMSA and ChIP assays showed that CREB protein interacts with the galanin promoter both in vitro and in vivo. Additional EMSA analysis revealed that an SP1 consensus site mediated protein-DNA complex formation. PMID: 24487089
    8. The expression of mRNA galanin is increased following chronic restraint stress. PMID: 23639882
    9. These results suggest that sertraline-related galanin induction in ventral dentate gyrus may play an important role in therapeutic mechanisms for depression. PMID: 23583289
    10. extended perifornical area galanin-LepRb neurons galanin acts inhibitory rather than orexigenic PMID: 23482448
    11. The results suggest that neither galanin expression, nor the galanin-dependent subpopulation of sensory neurons is required for itch-related behaviours. PMID: 23216829
    12. Galanin transcript expression increased significantly in male mouse major pelvic ganglia neurons after 72 h in explant culture & 72 h after unilateral transection of the cavernous nerve. PMID: 22585545
    13. results indicate normal cholinergic responses and innervation of the sweat glands in galanin knockout mice, they also demonstrate that galanin plays a role in regulating the sudomotor activity in response to thermal stimulation PMID: 22698811
    14. GAL is a promising next-generation target for the treatment of MS. PMID: 22442732
    15. Galanin opposes the actions of morphine which leads to opiate dependence and withdrawal, an effect that is mediated via GalR1 PMID: 21969124
    16. Subpopulations of kisspeptin neurons express galanin and met-enkephalin in the rostral periventricular region of the hypothalamus. PMID: 21800299
    17. These data showed that the galanin system is regulated by repeated electroconvulsive stimulation in brain regions involved in monoaminergic neurotransmission and stress modulation. PMID: 21820174
    18. examined the role of galanin signaling in nicotine reward by testing the effects of nicotine in mice lacking galanin peptide (GAL-/-) as compared to wild-type (GAL+/+) controls PMID: 21172385
    19. Galanin, a potent inhibitor of pancreatic bicarbonate secretion, is involved in the induction and progression of cerulein-induced experimental acute pancreatitis. PMID: 21160373
    20. Data show that the galanin system is regulated by repeated electroconvulsive stimulation in a number of brain regions implicated in seizure regulation and depression. PMID: 20936701
    21. results suggest the possible association of the galanin-mediated signaling pathways in the manifestation of the high-fat diet-induced activation of adipogenesis along with the suppression of thermogenesis in the adipose tissues PMID: 20183829
    22. Galanin is able to reduce neuropathic pain behaviour. PMID: 20964829
    23. Hypothalamic galanin peptides play a physiological role in stimulating the consumption of ethanol, as well as a fat-rich diet. PMID: 19860804
    24. Suggest a role for galanin in the pathogenesis of acute pancreatitis. PMID: 19959967
    25. These results obtained with overexpression of the GAL gene provide strong support for a physiological role of this peptide in stimulating the consumption of ethanol and a fat-rich diet. PMID: 20004335
    26. galanin knockout mice indicate that the peptide is critical to the development and function of neurons in the central and peripheral nervous system PMID: 11712539
    27. Galanin modulates anxiety states induced by high levels of noradrenaline;a specific role for galanin in anxiety states represents an attractive target for novel anxiolytic treatments. PMID: 11931346
    28. findings are the first demonstration that galanin impairs performance on an emotional memory task and support the hypothesis that galanin-induced deficits are specific to more difficult cognitive tasks PMID: 12177231
    29. upregulation of galanin in DRG neurones following nerve injury results in antinociception via stimulation of galanin GAL1 receptors and that the pro-nociceptive effect of galanin is related to GAL2 receptors on primary afferents--review PMID: 12368071
    30. Perinatal nicotine attenuates the hypoxia-induced up-regulation of galanin mRNA in locus ceruleus of the newborn mouse. PMID: 12409526
    31. neither hippocampal nor cortical galanin levels in the two cohorts of GAL-tg consistently correlated with performance across diverse cognitive tasks PMID: 12507436
    32. GAL inhibited TNF alpha release but not its mRNA levels in BV2 cells. GAL inhibits TNF alpha production by a post-transcriptional mechanism that both prevents the efficient addition of the poly(A) tail and accelerates TNF alpha mRNA degradation. PMID: 12507772
    33. Evidence is provided for an inhibitory function for the neuropeptide galanin acting on galanin receptor 1 (GALR1) in nociception and neuropathic pain after peripheral nerve injury in mice. PMID: 12605908
    34. data establish a new role for galanin as a hormone augmenting mammary development during pregnancy PMID: 12759342
    35. modulates behavioral and neurochemical signs of opiate withdrawal PMID: 12853567
    36. role of galanin in the regulation of release of NA and 5-HT in the hippocampus after forced swimming PMID: 14701907
    37. The unexpected obesity phenotype in galanin-neuropeptide Y double knockout mice may result from the dysregulation of the leptin and insulin systems that normally keep body weight within the homeostatic range. PMID: 15024085
    38. galanin may contribute to sympathetic influences inhibiting insulin secretion in mice, and galanin knockout mice have a reduced glucose-induced insulin secretion. PMID: 15044363
    39. Increased availability of galanin in spinal dorsal horn at the time or shortly after nerve injury may reduce the development of pain-like behaviors in mice. PMID: 15464755
    40. galanin may have a role in functions related to mood states including affective disorders PMID: 15582716
    41. mechanistic relationship between amyloidosis and GAL over expression in Alzheimer disease PMID: 15893372
    42. Lack of galanin-maintained cAMP tone in the galanin knockout mouse appears to result in a loss of negative feedback resulting in increased levels of CREB phosphorylation and increased GalR1 expression. PMID: 15934937
    43. estrogen receptors in trigeminal neurons modulate nociceptive responses through effects on galanin and NPY PMID: 15936815
    44. Mice overexpressing galanin had a moderate heat hypoalgesia, reduced spinal sensitization after repetitive C-fiber stimulation and reduced development of neuropathic pain-like behavior after sciatic nerve injury PMID: 15944013
    45. excess GAL in cognitive impairment is relevant to the overexpression of GAL in the basal forebrain during the progression of Alzheimer's disease PMID: 15944016
    46. galanin is involved in hippocampal synaptic plasticity, in particular in age-related reduction of synaptic plasticity in the lateral perforant path input to the dentate gyrus PMID: 15944020
    47. The highest levels of transgenic galanin expression were observed in the forebrain structures, in the mesencephalon, in the cerebellum, in the pons, and within the medulla oblongata PMID: 15944025
    48. galanin may have a role in functions related to mood states, including affective disorders PMID: 15944026
    49. overexpression of galanin can influence several behavioral and neurochemical parameters in old mice PMID: 15944027
    50. both Gal and NPY contribute to the prolonged attenuation of parasympathetic slowing of the heart following activation of the cardiac sympathetic nerve PMID: 15944033

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Galanin family
  • 数据库链接：

    KEGG: mmu:14419

    STRING: 10090.ENSMUSP00000025842

    UniGene: Mm.4655