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  4. Mouse Tumor necrosis factor ligand superfamily member 9(TNFSF9) ELISA kit

Mouse Tumor necrosis factor ligand superfamily member 9(TNFSF9) ELISA kit

  • 中文名称：
    小鼠肿瘤坏死因子配体超家族成员9(TNFSF9)酶联免疫试剂盒
  • 货号：
    CSB-EL023997MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse TNFSF9 ELISA Kit was designed for the quantitative measurement of Mouse TNFSF9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    Tnfsf9; Cd137l; Cd157l; Ly63l; Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.

A:
Thanks for your inquiry!
CSB-EL023997MO
This kit is designed against extracellular domain. Pls refer to: https://www.uniprot.org/uniprot/P41274.
Capture antibody is goat polyclonal antibody and detection antibody is goat polyclonal antibody.
Immunogen is 293 cell-derived recombinant protein.
Standard is 293 cell-derived recombinant protein.
Q:

“Regarding the cncentration of sulfuric acid in this MSDS.
Is 4N sulfuric acid 2mol/l?
What is the meaning of □ of 1□ x 10ml?”

A:
Yes 4N sulfuric acid is 2mol/L. Both units are ok.
The volume of sulfuric acid in the 96T kit is 10mL.
Q:

Per customers request, please provide the following information regarding Mouse Tumor necrosis factor ligand superfamily member 9, TNFSF9 ELISA Kit (CSB-EL023997MO)
1)The active ingredient of the stop solution and its concentration ?
2)The type, concentration, and state (liquid/lyophilized) of the preservatives used ?
3) If the components are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?
4)Expression host of the standards ?
5)Which components have H202 with the concentration of H202 in each, if any?
6) Are there any components that contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) in any of the components in this kit?
7) If yes, which components contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) and the concentration?

A:
Thanks for your inquiry!
CSB-EL023997MO
Standard：293 cell-derived recombinant protein（The preservatives in the standard is liquid proclin 300(1:3000)).
The standard is lyophilized, and the concentration after reconstitution is 1500pg/mL.
Stop solution is 4N sulfuric acid. ( There is no preservatives in it.)
This kit doesn't contain H202（hydrogen peroxide） & Urea hydrogen peroxide (Carbamide peroxide).
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
  • 基因功能参考文献：
    1. this study shows that intrinsic 4-1BB signals are indispensable for the establishment of an influenza-specific tissue-resident memory CD8 T-cell population in the lung PMID: 28051085
    2. 4-1BBL can restrain effector T cell development, creating a more favorable regulatory T cell to effector cell balance under tolerogenic conditions, and this may be particularly active in mucosal barrier tissues PMID: 25404362
    3. These results demonstrate that 4-1BBL-engineered DCs can improve CIKs cytotoxicity against prostate cancer cells. PMID: 25572476
    4. Data indicate that CD137L deficient mice displayed a variety of immunological dysfunctions. PMID: 24091276
    5. CD137-expressing CD4+ T cells in the bone marrow engage CD137L on hematopoietic progenitor cells, and this CD137L signaling biases hematopoiesis towards myelopoiesis during aging. PMID: 23945137
    6. Results suggest that CD137L reverse signaling exerts a pro-apoptotic effect by suppressing integrin-mediated survival signals in neural stem cells. PMID: 23925549
    7. A VCAM-1-positive stromal cell is a plausible candidate for the radioresistant cell that provides 4-1BB ligand to sustain memory CD8-positive T cells. PMID: 22886791
    8. The CD137L induces tyrosine phosphorylation as well as the up-regulation of M-CSF, IL-1beta and TN-C expressions by knockdown of TMEM126A. PMID: 22885069
    9. 4-1BB signaling negatively modulate Treg cells by two distinct mechanisms: i) inhibiting the conversion of CD4(+)FoxP3(-) T cells into iTreg cells and ii) endowing Teff cells refractory to inhibition by Treg cells. PMID: 22870329
    10. The CD137L reverse signaling pathway in epithelial cells may represent a good target for blocking the initial stage of inflammatory diseases, including renal ischemia-reperfusion injury. PMID: 22160719
    11. Reverse signals initiated by CD137L negatively modulate certain immune functions of thioglycollate-elicited peritoneal macrophages PMID: 21184130
    12. Mice inoculated with H22 tumor cells expressing B7-1, B7-2 and 4-1BBL developed a strong cytotoxic T lymphocyte response and long-term immunity against wild-type tumor, suggesting a synergistic effect between the B7 and 4-1BBL costimulatory pathways PMID: 20563597
    13. Cocultures of Natural killer (NK) cells with CD137L transfectants confirmed that human CD137 inhibits NK-cell reactivity, while activating signals were transduced by its counterpart on NK cells in mice. PMID: 20008791
    14. A role for 4-1BB ligand in dendritic cell activation PMID: 11867564
    15. In a murine model, herpetic stromal keratitis is inhibited by blockade of 4-1BBL/4-1BB interactions with monoclonal antibodies to 4-1BBL or by deletion of 4-1BB. PMID: 12847221
    16. involvement of 4-1BB in CD4(+) Th1 cell responses by regulating the clonal expansion and survival of CD4(+) T cells as seen in CD8(+) T cells PMID: 14749528
    17. Characterization of the role of 4-1BBL in activation and recall response of CD4 T cells vs CD8 T cells. PMID: 14991604
    18. demonstrate that suppression of experimental autoimmune uveoretinitis results from antigen-driven, 4-1BB-mediated expansion of novel CD11c+CD8+ T cells that suppress antigen-specific CD4+ T cells via an indole 2,3-dioxygenase-dependent mechanism PMID: 16899371
    19. indicate that the CD28 costimulatory pathway plays a major role in the alloimmune response and that 4-1BB signals are dependent upon CD28 signals PMID: 17202836
    20. IL-13 was coinduced following 4-1BB triggering to maintain the Th1/2 balance of immune response. PMID: 17389581
    21. Thus, long-term inhibition of the 4-1BB pathway reduces cardiac damage, remodeling, and inflammation during viral myocarditis. PMID: 17468777
    22. CD137 ligand plays a role in allergic asthma. PMID: 17845419
    23. CD80 and 4-1BBL induce auto- and transcostimulation in tumor cells PMID: 18026115
    24. deliver new insights into the multiple effects of reverse signaling of CD137L in human DC during the initiation of an adaptive immune response PMID: 18395851
    25. IL12- and CD137L-transfected plasmocytoma cells prevented tumor growth and induced long-lasting immunity PMID: 18610746
    26. Reverse CD137 ligand signaling takes place in hematopoietic progenitor cells, where it induces proliferation and differentiation toward monocytes and macrophages. PMID: 18768847
    27. modification of cancer cells with 4-1BBL gene can produce anti-tumor immune responses. PMID: 18954562
    28. During mild respiratory influenza infection in which virus is rapidly cleared, costimulatory receptor 4-1BB is transiently induced on lung T cells and 4-1BB ligand (4-1BBL) is completely dispensable for the initial CD8 T cell response and mouse survival. PMID: 19124736
    29. 41BBL requires hexamerization for activation PMID: 19596991
    30. CD137L ordinarily regulates the GC B-cell response and thereby acts as a tumor suppressor. PMID: 19608748
    31. These findings demonstrate that reverse signals evoked by CD137L regulate immune functions in macrophages. PMID: 19676073
    32. 4-1BB ligand induces cell division, sustains survival, and enhances effector functions of CD4 and CD8 T cells under comparable conditions of antigenic stimulation. PMID: 11466348

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  • 亚细胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：