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  4. Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit

Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit

  • 中文名称：
    小鼠单核细胞趋化蛋白5(MCP-5)酶联免疫试剂盒
  • 货号：
    CSB-E15766m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        MCP-5（Monocyte chemoattractant protein-5）是一种由单核细胞产生的趋化因子，它可以诱导单核细胞和T细胞的趋化。在炎症和免疫应答过程中，MCP-5的含量通常会升高，其正常范围因人而异，但超过正常范围可能与炎症和免疫疾病相关。
        j9九游会登录入口首页生物所提供的Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的MCP-5，其灵敏度为16.82 pg/mL，检测范围为15.6 pg/mL-1000 pg/mL。
     
  • 别名：
    C-C motif chemokine 12 ELISA Kit; Ccl12 ELISA Kit; CCL12_MOUSE ELISA Kit; Macrophage/monocyte Chemotactic Protein ELISA Kit; MCP-1-related chemokine ELISA Kit; MCP-5 ELISA Kit; Monocyte chemoattractant protein 5 ELISA Kit; Monocyte chemotactic protein 5 ELISA Kit; OTTMUSP00000000792 ELISA Kit; Scya12 ELISA Kit; Small inducible cytokine A12 ELISA Kit; Small-inducible cytokine A12 ELISA Kit
  • 缩写：
    MCP-5
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    16.82 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 87    
    Range % 82-91    
    1:2 Average % 94    
    Range % 89-98    
    1:4 Average % 107    
    Range % 103-110    
    1:8 Average % 110    
    Range % 104-114    
  • 回收率：
    The recovery of mouse MCP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 91-99    
    EDTA plasma (n=4) 103 98-108    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.615 2.634 2.625 2.499    
    500 2.177 2.012 2.095 1.969    
    250 1.432 1.502 1.467 1.341    
    125 0.842 0.839 0.841 0.715    
    62.5 0.531 0.529 0.530 0.404    
    31.2 0.334 0.326 0.330 0.204    
    15.6 0.246 0.251 0.249 0.123    
    0 0.125 0.127 0.126 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    MCP-5是一种趋化因子，全名为单核细胞趋化蛋白5。它主要参与免疫细胞的趋化和活化过程，在炎症、感染、肿瘤等疾病的发生和发展中发挥重要作用。最新研究发现，MCP-5参与了多种肿瘤的发生和进展，同时也是一种重要的治疗靶点。
  • 功能：
    Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
  • 基因功能参考文献：
    1. After 100% O2 treatment, genes involved in inflammation (Ccl12), angiogenesis (Igfr1, Stat3), and metabolism (Hk2) were upregulated. PMID: 24458145
    2. Data indicate mRNA upregulation of CCR2 and CCR2 ligands CCL2, CCL7, CCL8, and CCL12 in aortas from DOCA/salt-treated hypertension. PMID: 23033370
    3. The transforming growth factor (TGF)beta type II receptor/MCP-5 axis is an essential crossroad for joint development and endochondral growth. PMID: 22814601
    4. CCL12 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 组织特异性：
    Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
  • 数据库链接：

    KEGG: mmu:20293

    STRING: 10090.ENSMUSP00000000194

    UniGene: Mm.867