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  4. Mouse Metallothionein-2(MT-2) ELISA Kit

Mouse Metallothionein-2(MT-2) ELISA Kit

  • 中文名称：
    小鼠金属硫蛋白2(MT-2)酶联免疫试剂盒
  • 货号：
    CSB-E13693m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse MT-2 ELISA Kit was designed for the quantitative measurement of Mouse MT-2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Mt2 ELISA Kit; Metallothionein-2 ELISA Kit; MT-2 ELISA Kit; Metallothionein-II ELISA Kit; MT-II ELISA Kit
  • 缩写：
    MT-2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MT-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %96
    Range %91-102
    1:2Average %102
    Range %99-105
    1:4Average %89
    Range %86-93
    1:8Average %94
    Range %92-97
  • 回收率：
    The recovery of mouse MT-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 103101-106
    EDTA plasma (n=4)9592-109
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10001.975 2.022 1.999 1.842
    5001.362 1.377 1.370 1.213
    2500.811 0.862 0.837 0.680
    1250.433 0.439 0.436 0.279
    62.50.304 0.330 0.317 0.160
    31.20.262 0.274 0.268 0.111
    15.60.214 0.220 0.217 0.060
    00.163 0.150 0.157
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
  • 基因功能参考文献：
    1. these results demonstrate that MT2 plays a role in CD4+ T cell activation by transducing reactive oxygen species signals into an increased [Zn2+]i that subsequently affects downstream effector function PMID: 27251638
    2. Metallothioneins may have a protective role against post-burn inflammation and inflammatory organ damage, at least partly through inhibiting the p38 MAPK signaling. PMID: 26833479
    3. The MT2 receptor activates Akt/GSK-3beta/CRMP-2 signaling and is necessary and sufficient to mediate functional axonogenesis and synaptic formation in central neurons. PMID: 25501601
    4. Zn deficiency in utero induces fetal epigenetic alterations of Mt2 gene and these changes are being stored as an epigenetic memory until adulthood. PMID: 22917840
    5. Knockdown of metallothionein 2 does not affect atrophy or oxidant activity in skeletal myotubes subject to serum reduction. PMID: 20798270
    6. MT-null phenotype precludes lead-induced inclusion body formation and increases lead toxicity at the organ and cellular level despite reducing lead accumulation PMID: 11891201
    7. MT-2 deficiency increases brain pathology in transgenic mice with astrocyte-targeted expression of interleukin 6. PMID: 11950277
    8. In mouse spinal cord injury lead the up-regulation MT-2 expression at 1 day and MT-2 expression was observed in activated macrophages/microglia at 1 day. PMID: 15592854
    9. MT2a has a role in oxidative stress, and the formation of MT disulfide bonds is involved in the regulation of zinc homeostasis PMID: 16243847
    10. Mt-2 synthesis was induced by cilostazol. PMID: 17409522
    11. Metallothionein II deficient mice exhibit impaired rate of maze learning but retained the information as well as the wild-type mice. The absence of functional MT-II does not result in significantly worse injury following 45 min of hypoxia-ischemia. PMID: 18083145
    12. During the early phase of learning metallothionein-2 null mice were more susceptible to mercury-induced impairment compared to wildtype mice PMID: 18226494

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  • 蛋白家族：
    Metallothionein superfamily, Type 1 family
  • 数据库链接：