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  4. Mouse Immunoglobulin M,IgM ELISA Kit

Mouse Immunoglobulin M,IgM ELISA Kit

  • 中文名称：
    小鼠免疫球蛋白M(IgM)酶联免疫试剂盒
  • 货号：
    CSB-E07977m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse IgM ELISA Kit was designed for the quantitative measurement of Mouse IgM protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 62.5 ng/mL.

  • 缩写：
    IgM
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    62.5 ng/mL-4000 ng/mL
  • 灵敏度：
    62.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 93  
    Range % 88-94  
    1:2000 Average % 94  
    Range % 90-98  
    1:4000 Average % 89  
    Range % 85-94  
    1:8000 Average % 97  
    Range % 91-101  
  • 回收率：
    The recovery of mouse IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-96  
    EDTA plasma (n=4) 103 98-107  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    4000 0.353 0.382 0.368    
    1000 0.459 0.440 0.450    
    500 0.541 0.507 0.524    
    250 0.658 0.672 0.665    
    62.5 1.198 1.114 1.156    
    0 1.964 1.903 1.934    
  • 数据处理：
  • 货期：
    3-5 working days

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