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  4. Mouse Fibrinogen-like protein 1(FGL1) ELISA kit

Mouse Fibrinogen-like protein 1(FGL1) ELISA kit

  • 中文名称：
    小鼠纤维蛋白原样蛋白1(FGL1)酶联免疫试剂盒
  • 货号：
    CSB-EL008653MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse FGL1 ELISA Kit was designed for the quantitative measurement of Mouse FGL1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    Fgl1 ELISA Kit; Mfire1 ELISA Kit; Fibrinogen-like protein 1 ELISA Kit; Fibrinogen-related protein 1 ELISA Kit; Mfrep-1 ELISA Kit; Liver fibrinogen-related protein-1 ELISA Kit; Mfire-1 ELISA Kit
  • 缩写：
    FGL1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %95
    Range %91-99
    1:200Average %84
    Range %81-89
    1:400Average %83
    Range %90-96
    1:800Average %87
    Range %84-90
  • 回收率：
    The recovery of mouse FGL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 104100-108
    EDTA plasma (n=4)107104-110
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2Average
    20000.076 0.079 0.078
    10000.135 0.146 0.141
    5000.206 0.220 0.213
    2500.362 0.379 0.371
    1250.593 0.586 0.590
    62.50.901 0.932 0.917
    31.251.213 1.236 1.225
    02.054 2.098 2.076
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3. Responsible for LAG3 T-cell inhibitory function. Binds LAG3 independently from MHC class II (MHC-II). Secreted by, and promotes growth of, hepatocytes.
  • 基因功能参考文献：
    1. The results suggest that Fgl1 acts as a tumor suppressor in hepatocellular cancer through an Akt-dependent mechanism and support its role as a potential therapeutic target in hepatocellular carcinoma. PMID: 26225745
    2. Hepassocin plays an important role in non-alcoholic fatty liver disease and induces hepatic lipid accumulation through an ERK1/2-dependent pathway. PMID: 23792031
    3. Fgl1 is a member of an emerging group of proteins with key roles in metabolism and liver regeneration. PMID: 23483972
    4. HNF1 binding site and HNF1alpha are critical to liver-specific expression of HPS, and down-regulation or loss of HNF1alpha causes, at least in part, the transcriptional down-regulation of HPS in HCC. PMID: 19304666
  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Mainly expressed in liver. Also expressed in brown adipose tissue.
  • 数据库链接：