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Mouse E-Selectin ELISA kit

  • 中文名称：
    小鼠E选择素(E-Selectin/CD62E)酶联免疫试剂盒
  • 货号：
    CSB-E04541m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        SELE（E-selectin）：SELE是一种细胞黏附分子，属于血管内皮细胞黏附分子家族，主要通过与其上游调控区的L-选择素（L-selectin）和P-选择素（P-selectin）结合，参与炎症和免疫反应中白细胞的黏附和转移。SELE的表达受多种因素的调控，如TNF-α、IL-1、LPS等。在某些疾病状态中，SELE的表达会增加，因此SELE也被认为是一种炎症指标。
        j9九游会登录入口首页生物所提供的Mouse E-Selectin ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的SELE，其灵敏度为19.5 pg/mL，检测范围为78 pg/mL-5000 pg/mL。
     
  • 别名：
    Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse E-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 90-96    
    1:2 Average % 85    
    Range % 81-90    
    1:4 Average % 104    
    Range % 100-108    
    1:8 Average % 86    
    Range % 82-90    
  • 回收率：
    The recovery of mouse E-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 102 98-106    
    EDTA plasma (n=4) 89 85-94    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    5000 2.076 2.131 2.104 1.907    
    2500 1.671 1.638 1.655 1.458    
    1250 1.168 1.201 1.185 0.988    
    625 0.829 0.831 0.830 0.633    
    312 0.505 0.511 0.508 0.311    
    156 0.365 0.372 0.369 0.172    
    78 0.285 0.289 0.287 0.090    
    0 0.195 0.199 0.197 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    SELE（血管内皮细胞选择素）参与白细胞的粘附和迁移。
  • 功能：
    Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
  • 基因功能参考文献：
    1. Before the arrival of tumor cells, the lung-recruited monocytic myeloid-derived suppressor cells produced IL-1beta, thereby increasing E-selectin expression and promoting tumor cell arrest on endothelial cells. PMID: 27885671
    2. Inhibiting E-selectin attenuated inflammation in atherosclerotic plaques, likely by reducing leukocyte recruitment into plaques and by mitigating hematopoietic stem and progenitor cell activation in the spleen of mice with myocardial infarction. PMID: 27470513
    3. In a model of corneal transplantation, P- and E-selectin mediate T cell recruitment to the graft, E-selectin mediates APC trafficking to lymphoid tissue, and blockade of E-selectin has a modest effect on improving long-term graft survival. PMID: 26910325
    4. Complex spatiotemporal expression dynamics of selectins determine the sequential extravasation of neutrophils and inflammatory monocytes in the acute inflammatory response. PMID: 25722429
    5. involved in neutrophil recruitment into the kidney after induction of sepsis PMID: 24576991
    6. The histopathological modification of lupus nephritis by non-nephritogenic bystander IgM antibodies is associated in part with glomerular E-selectin expression. PMID: 24372297
    7. Data show that actin cytoskeleton regulated CD44 membrane mobility and interactions with E-selectin. PMID: 25359776
    8. Regulatory T cells dynamically regulate P and E selectin ligand expression during multiple challenge contact hypersensitivity. PMID: 25274531
    9. Systemic inflammation may increase the expression of E-selectin which mediated the lung metastasis of breast cancer in mouse model. PMID: 24658642
    10. In the absence of either PSGL-1 or E- and P-selectin. PMID: 24740164
    11. The study suggest that E- and P-selectins play an important role in leukemic dissemination in chronic myelogenous and chronic eosinophilic leukemias. PMID: 23922938
    12. Elevated E-selectin plays an important role in hepatic neutrophil infiltration and injury induced by chronic-binge feeding in mice and may also contribute to the pathogenesis of early stages of human alcoholic liver disease. PMID: 23532958
    13. expression of E-selectin and ICAM-1 are suppressed by paeoniflorin in a mouse model of cutaneous Arthus reaction PMID: 23800055
    14. Expression of E-selectin is sufficient to support rescue & hematopoietic reconstitution of lethally irradiated recipients forllowing bone marrow transplant. PMID: 23499761
    15. TWEAK acted as an inducer of E-selectin and ICAM-1 expression in mice with cutaneous vasculitis and human dermal microvascular endothelial cells PMID: 23457623
    16. E-selectin deficiency presents a protective effect on cerebral ischemia, likely achieved by the inhibition of inflammation and apoptosis. PMID: 22978829
    17. Constitutively expressed E-selectin on endothelial cells in the proliferating phase is one mediator of the stem cell tropism in infantile hemangioma. PMID: 23041613
    18. beta(3)-adrenergic stimulation acts through up-regulation of E-selectin in adipose tissue endothelial cells to induce neutrophil infiltration PMID: 23235150
    19. transient blockade of E-selectin is potentially a promising treatment for the protection of HSCs during chemotherapy or irradiation PMID: 23086476
    20. the up-regulation of P-selectin, E-selectin and intercellular adhesion molecule-1 contributes to the increased leucocyte rolling flux, reduced leucocyte rolling velocity, and increased leucocyte adhesion, respectively. PMID: 22681228
    21. Data show that the protective effect of hydroxyurea was abrogated in mice deficient in E-selectin. PMID: 22130804
    22. Primary subcutaneous growth of B16 melanoma was augmented by loss of PSGL-1, P-selectin, or E-selectin, while pulmonary metastasis was reduced by the loss of E-selectin. PMID: 21889879
    23. Localized activation of endothelial FAK and E-selectin in the lung vasculature mediates the initial homing of metastatic cancer cells to specific foci in the lungs. PMID: 21321210
    24. lack of alpha4beta7 or E-selectin significantly reduced trafficking of CD4 T cells to the GT of mice infected with C. muridarum. PMID: 19392981
    25. E-selectin and P-selectin mediate the recruitment of reperfusion-induced bone marrow stromal cells to the infarct site and enable their adhesion to the cerebral microvasculature following ischemia. PMID: 21257828
    26. Immature mast cells can undergo a tightly regulated extravasation cascade consisting of rolling on and adhesion to endothelium and followed by directed diapedesis and reveal E-selectin, P-selectin, VCAM-1 and PECAM-1 as required adhesion molecules. PMID: 20507363
    27. No major differences were identified between ICAM-1 and E-selectin expression in varicose vein specimens compared with controls. PMID: 20632821
    28. E-selectin triggers common signals through distinct neutrophil glycoproteins to induce alpha(L)beta(2)-dependent slow rolling PMID: 20299514
    29. core 1-derived O-glycans are essential E-selectin ligands, while PSGL-1, CD44, and ESL-1 do not constitute all glycoprotein ligands for E-selectin. PMID: 20439727
    30. Embryonic trafficking of gammadelta T cells to skin is dependent on E/P selectin ligands and CCR4 PMID: 20368416
    31. The signaling pathway downstream of Syk divides into PLC2- and PI3K-dependent pathways. The activation of these pathways is controlled by the Tec family kinase Btk, which is required for E-selectin-mediated slow rolling. PMID: 20167705
    32. E- and P-selectins have a crucial role in spontaneous metastasis formation. PMID: 20010946
    33. E-selectin seems to function in the formation of tubelike structures in vascular endothelial cell cultures in angiogenesis. PMID: 11880280
    34. critical role of carbohydrate antigens and E-selectin in metastasis, and peptides mimicking carbohydrate antigens can function as antagonists of this process PMID: 12084533
    35. contributes to CRA-induced peribronchial inflammation and airway hyperreactivity. PMID: 12165540
    36. E-selectin-mediated lymphocyte accumulation in the lungs following intratracheal challenge with sheep red blood cells in primed animals is subset and time dependent. PMID: 12193727
    37. The migration of in-vitro generated cytotoxic T lymphocytes into inflamed skin is partially mediated by E-selectin. PMID: 12370362
    38. Bone marrow cells from E-selectin knockout mice do not show altered megakaryocytopoiesis. PMID: 12370396
    39. A dysfunctional immune response in E-selectin knockout mice lacking also P-selectin results from impaired leukocyte trafficking and leads to chronic ulcerative dermatitis. PMID: 12391189
    40. CLA/E-selectin interactions plays a role in CCR4-mediated recruitment of human Th2 memory cells to human skin in vivo. PMID: 12555662
    41. study demonstrates a difference in fibrin content of thrombi produced in animals deficient in E-selectin, P-selectin, and IL-10, supporting their importance in thrombus amplification, fibrin formation, and the mass of thrombus formed PMID: 12591228
    42. testicular ischemia-reperfusion injury stimulates IL-1beta expression, leading to activation of the JNK signaling pathway and ultimately E-selectin expression and neutrophil recruitment to the testis PMID: 12620934
    43. Results suggest that CD4 T cells from lymph nodes enter the site of antigen deposition via recognition of CD62E on blood vessels and are retained there in a nonproliferative state via recognition of peptide-major histocompatibility complex II molecules. PMID: 12629067
    44. This protein contributes to tissue injury induced by immune complex deposition. PMID: 12707029
    45. major difference between mature myeloid cells and immature stem/progenitor cells in that E-selectin ligands cooperate with alpha4 integrin rather than P-selectin ligands. PMID: 12763924
    46. Data show that both P- and E-selectin play an important role in Th1 lymphocyte migration to lung, and that the Th1 cells express P-selectin glycoprotein ligand-1, which was functional in binding to P- and E-selectin in vitro. PMID: 12920054
    47. E-selectin showed a transient induction of expression peaking between 2.5 to 4 hours and declining thereafter. While E-selectin was expressed, rolling could only be eliminated by combined blockade of P- and E-selectin. PMID: 14507656
    48. E-selectin is not required for the expression of host-protective immunity against cutaneous infection with Leishmania major. PMID: 14568948
    49. E-selectin has a role in promoting growth inhibition and apoptosis of human and murine hematopoietic progenitor cells independent of PSGL-1. PMID: 14592840
    50. expression at mRNA and protein levels in inflammation PMID: 14991080

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Selectin/LECAM family
  • 数据库链接：

    KEGG: mmu:20339

    STRING: 10090.ENSMUSP00000027874

    UniGene: Mm.5245