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  4. Mouse Cystatin C,Cys-C ELISA Kit

Mouse Cystatin C,Cys-C ELISA Kit

  • 中文名称：
    小鼠半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)酶联免疫试剂盒
  • 货号：
    CSB-E08386m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        CST3是一种半胱氨酸蛋白酶抑制剂，也被称为半胱氨酸蛋白酶C抑制剂。CST3可以在多种组织和液体中发现，具有许多生物学功能，包括细胞增殖、细胞凋亡和炎症调节等方面的作用。
        j9九游会登录入口首页生物所提供的Mouse Cystatin C,Cys-C ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的CST3，其灵敏度为0.819 ng/mL，检测范围为2.5 ng/mL-160 ng/mL。
     
  • 别名：
    Cst3Cystatin-C ELISA Kit; Cystatin-3 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.5 ng/mL-160 ng/mL
  • 灵敏度：
    0.819 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

     Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess. 
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess. 
  • 线性度：

     To assess the linearity of the assay, samples were spiked with high concentrations of mouse Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

  • 标准曲线：

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    CST3也被称为半胱氨酸蛋白酶抑制剂或肾素抑制剂。它是一种广泛存在于生物体中的囊泡蛋白，可作为生物标记物用于疾病的诊断和预后评估。最近的研究表明，CST3可能在心血管疾病、神经退行性疾病、肿瘤、糖尿病等疾病的发生和发展中发挥重要作用，因此被认为是潜在的治疗靶点。此外，一些研究表明CST3在肾脏疾病中发挥重要作用，因为它与肾小管损伤和肾功能下降相关。
     
  • 功能：
    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • 基因功能参考文献：
    1. CysC plays an important regulatory role in combating cell death via the autophagic pathway in atherosclerosis. PMID: 27079462
    2. Data (including data from studies in mutant mice) suggest that up-regulation of Cst3, as observed in plasma of mice with type 2 diabetes, down-regulates insulin signaling and promotes endoplasmic reticulum stress in hepatocytes but not in myotubes. PMID: 26592151
    3. Cystatin C is a potential pathogenic signal triggering neurodegeneration in multiple system atrophy. PMID: 24405769
    4. The neuroprotective activity of CysC against Amyotrophic lateral sclerosis-linked mutant Cu/Zn-superoxide dismutase (SOD1)-mediated toxicity. PMID: 25356866
    5. an important role for macrophages, DC, and ROS in diseases associated with the protease inhibitory activity or amyloidogenic properties of cystatin C. PMID: 24570004
    6. APP expression stimulates NSPC proliferation; this effect is mediated via an increase in cystatin C secretion PMID: 23671283
    7. Cystatin C, a protein targeted to the classical secretory pathway by its signal peptide sequence, is secreted by primary neurons in at least 9 different cystatin C glycoforms associated with exosomes. PMID: 19773092
    8. The lack of cystatin C enhances Collagen-induced arthritis and primarily affects in vivo priming of the immune system PMID: 21443774
    9. Inflammation causes downregulation of cystatin C expression in dendritic cells and reduces serum CstC levels. PMID: 21300820
    10. Data show that the absence of cystatin C reduced epithelial cell apoptosis but increased proliferation in skin. PMID: 21085595
    11. Data show that cystatin C effectively rescues cystatin B loss-of-function mutation, facilitating the reversal of pathophysiological changes and suggesting a novel therapeutic intervention for patients with neurodegenerative disorders. PMID: 20889561
    12. CysC plays a protective role under conditions of neuronal challenge by inducing autophagy via mTOR inhibition PMID: 20352108
    13. Oxidants induce elevated cystatin C production from cardiac myocytes. Cystatin C plays a role in cardiac extracellular matrix remodelling. PMID: 20489058
    14. Data show that cystatin c (CystC) contributes to experimental abdominal aortic aneurysms (AAA) pathogenesis and that lack of CystC favors inflammation in AAA lesions induced in atherosclerotic mice. PMID: 20472891
    15. The embryo's cystatin C and F expression functions as a protective mechanism against the maternal proteinase cathepsin S. PMID: 20093401
    16. Cystatin C through this effect can act as an immunomodulatory molecule. PMID: 19446036
    17. expressed in decidual cultures and the expression is controlled in part by TGF-beta(1) and EGF signaling PMID: 11803549
    18. spatiotemporal up-regulation of cystatin C in the hippocampus is induced by entorhinal deafferentation (perforant path transections) PMID: 12044447
    19. Cystatin C is neither necessary nor sufficient to control expression of major histocompatibility type II complexes and antigen presentation in dendritic cells. PMID: 14607896
    20. In vivo overexpression of human cystatin C does not affect amyloid-beta levels in mice that do not deposit amyloid-beta. PMID: 14742906
    21. Data indicate that cystatin C is involved in astrocyte differentiation during mouse brain development. PMID: 15509901
    22. leukocyte-specific expression of cystatin C is actively involved in matrix remodelling associated with atherosclerosis plaque regression. PMID: 15823274
    23. the protective role of cystatin C in atherosclerosis is dependent primarily on its expression in nonhematopoietic cell types PMID: 16051881
    24. PKB activity is a critical downstream component of PDK-1 in mediating translation of cystatin C, RANKL, and Rab11a PMID: 16166629
    25. These data demonstrate that cystatin C has a role in neuronal death and neurogenesis during SE-induced network reorganization. PMID: 16242633
    26. Validity of cystatin C for generating neural stem cells from embryo stem cells in vitro. PMID: 16595632
    27. We propose that in MS, remyelination may be impaired by increasing activity of cathepsins inadequately controlled by cystatin C. PMID: 17086443
    28. Finally, in vivo studies using kidney-specific megalin knockout mice provide evidence that megalin mediates proximal tubular uptake of cystatin C. We conclude that megalin is an endocytic receptor of cystatin C in PTC. PMID: 17462596
    29. These results suggest that CysC affects the BMP signaling cascades in osteoblastic cells and then promotes osteoblast differentiation, mineralization, and bone formation. PMID: 17592728
    30. Plasminogen activator inhibitor-2, but not cystatin C, inhibits the prometastatic activity of tissue inhibitor of metalloproteinases-1 in the liver.( PMID: 18681831
    31. CysC regulates soluble Abeta and Abeta-associated neuronal deficits through inhibiting CatB-induced Abeta degradation. PMID: 18957217

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Cystatin family
  • 数据库链接：

    KEGG: mmu:13010

    STRING: 10090.ENSMUSP00000028938

    UniGene: Mm.4263