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  4. Human upper zone of growth plate and cartilage matrix associated (UCMA) ELISA kit

Human upper zone of growth plate and cartilage matrix associated (UCMA) ELISA kit

  • 中文名称：
    人生长板上部区域与软骨基质相关蛋白(UCMA)酶联免疫试剂盒
  • 货号：
    CSB-EL025550HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human UCMA ELISA Kit was designed for the quantitative measurement of Human UCMA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    UCMA ELISA Kit; C10orf49 ELISA Kit; Unique cartilage matrix-associated protein [Cleaved into: Unique cartilage matrix-associated protein C-terminal fragment ELISA Kit; Ucma-C ELISA Kit; Gla-rich protein ELISA Kit; GRP)] ELISA Kit
  • 缩写：
    UCMA
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Developmental Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human UCMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %82-91
    1:2Average %95
    Range %91-99
    1:4Average %96
    Range %93-99
    1:8Average %87
    Range %82-93
  • 回收率：
    The recovery of human UCMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8481-89
    EDTA plasma (n=4)8380-86
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.578 2.596 2.587 2.394
    52.168 2.115 2.142 1.949
    2.51.536 1.512 1.524 1.331
    1.250.969 0.958 0.964 0.771
    0.6250.598 0.572 0.585 0.392
    0.3120.455 0.456 0.456 0.263
    0.1560.371 0.398 0.385 0.192
    00.195 0.190 0.193
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface.
  • 基因功能参考文献：
    1. GRP might act as a novel molecular mediator linking inflammation and calcification events PMID: 28542410
    2. The study demonstrates the involvement of GRP in the two major molecular processes affecting osteoarthritis: mineralization and inflammation of articular tissue. PMID: 26337479
    3. Data suggest that novel splice variants of GRP are expressed in cartilage and other tissues (i.e. GRP-F5, GRP-F6); GRP-F1 is predominant splice variant expressed in adult tissues particularly in cartilage of patients with osteoarthritis. PMID: 24867294
    4. full exon resequencing of DNA samples from 17 chronic kidney disease patients (stage 5) and 121 healthy controls in a Swsedish population; the 138Thr > Ser polymorphism seems to be the only non-synonymous SNP found in the UCMA gene in a Swedish population PMID: 24409676
    5. Gla-rich protein (GRP) is a new vitamin K-dependent protein that can bind calcium through Gla residues. PMID: 18836183
    6. The present data strongly suggest an important function of Ucma in the early phase of chondrocyte differentiation. PMID: 17707622
    7. Ucma may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells PMID: 18156182

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    UCMA family
  • 组织特异性：
    Predominantly expressed in resting chondrocytes.
  • 数据库链接：

    HGNC: 25205

    KEGG: hsa:221044

    STRING: 9606.ENSP00000367952

    UniGene: Hs.112998