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  4. Human protein Red (IK) ELISA kit

Human protein Red (IK) ELISA kit

  • 中文名称：
    人RED蛋白(IK)酶联免疫试剂盒
  • 货号：
    CSB-EL011570HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human protein Red (IK) ELISA Kit was designed for the quantitative measurement of Human protein Red (IK) protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名：
    chondrosarcoma associated protein 2 ELISA Kit; CSA2 ELISA Kit; Cytokine IK ELISA Kit; IK ELISA Kit; IK cytokine down regulator of HLA II ELISA Kit; IK factor ELISA Kit; IK Gene ELISA Kit; prer protein ELISA Kit; Protein Red ELISA Kit; Protein RER ELISA Kit; RD element ELISA Kit; RED protein ELISA Kit; RED_HUMAN ELISA Kit; RER ELISA Kit
  • 缩写：
    IK
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    25 pg/mL-1600 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 82-94
    1:2 Average % 101
    Range % 98-104
    1:4 Average % 92
    Range % 85-96
    1:8 Average % 98
    Range % 94-102
  • 回收率：
    The recovery of human IK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 85-94
    EDTA plasma (n=4) 93 86-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 2.909 2.965 2.937 2.763
    800 2.215 2.332 2.274 2.100
    400 1.462 1.487 1.475 1.301
    200 0.900 0.923 0.912 0.738
    100 0.555 0.564 0.560 0.386
    50 0.363 0.378 0.371 0.197
    25 0.243 0.249 0.246 0.072
    0 0.173 0.174 0.174 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in pre-mRNA splicing as a component of the spliceosome. Auxiliary spliceosomal protein that regulates selection of alternative splice sites in a small set of target pre-mRNA species. Required for normal mitotic cell cycle progression. Recruits MAD1L1 and MAD2L1 to kinetochores, and is required to trigger the spindle assembly checkpoint. Required for normal accumulation of SMU1.; (Microbial infection) Required, together with SMU1, for normal splicing of influenza A virus NS1 pre-mRNA, which is required for the production of the exportin NS2 and for the production of influenza A virus particles. Not required for the production of VSV virus particles.
  • 基因功能参考文献：
    1. IK inhibits Aurora B activation through recruiting PP2A into IK and Aurora B complex. PMID: 26906715
    2. Data suggest an indirect radiosensitivity of hIK channels to low-dose photon irradiation with an impact on cell differentiation. PMID: 25277267
    3. Data suggest that two human spliceosomal factors, RED and SMU1, are recruited to control expression and alternative splicing of viral exportins via PB2 (polymerase PB2) of influenza A virus (H3N2) and are required for efficient viral multiplication. PMID: 24945353
    4. IK is an essential protein for achieving correct mitotic progress through the regulation of mitotic kinases and phosphatases PMID: 24996188
    5. RED is required for kinetochore localization of MAD1, mitotic progression, and activation of the spindle assembly checkpoint. PMID: 22351768
    6. IK gene expression is decreased in follicular variant of papillary thyroid carcinoma. PMID: 21509594
    7. At least three genes, orthologous to loci in this LD block, HBEGF, IK, and SRA1, result independently in a phenotype of myocardial contractile dysfunction when their expression is reduced with morpholino antisense reagents. PMID: 19064678

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleoplasm. Chromosome. Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族：
    RED family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 5958

    OMIM: 600549

    KEGG: hsa:3550

    STRING: 9606.ENSP00000396301

    UniGene: Hs.421245