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  4. Human placenta lactogen,HPL ELISA Kit

Human placenta lactogen,HPL ELISA Kit

  • 中文名称：
    人胎盘催乳素(HPL)酶联免疫试剂盒
  • 货号：
    CSB-E09665h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human placenta lactogen (HPL) ELISA Kit is used to quantitatively measure human HPL concentrations in serum or plasma. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of HPL in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

    HPL (CSH1) is a peptide hormone secreted throughout pregnancy by syncytiotrophoblast cells in humans. It mainly functions to regulate maternal lipid and carbohydrate metabolism and is important for mother and fetus energy homeostasis. Indeed, HPL facilitates lipolysis of human adipose tissue in-vitro, increasing the circulating free fatty acids. These can be used as an energy source by producing ketone bodies, which act as fuel for the fetus. HPL also plays an important role in the regulation of insulin secretion in pancreatic β-cells, stimulating their proliferation, and promoting the expression of anti-apoptotic proteins. HPL mainly binds to prolactin receptors and with a much lower affinity to growth hormone receptors.

  • 别名：
    CSH1Chorionic somatomammotropin hormone 1 ELISA kit; Choriomammotropin ELISA kit; Lactogen ELISA kit; Placental lactogen ELISA kit; PL ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    80 ng/mL-4000 ng/mL
  • 灵敏度：
    100 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %85-100
    1:2Average %96
    Range %90-105
    1:4Average %97
    Range %92-105
    1:8Average %95
    Range %89-100
  • 回收率：
    The recovery of human HPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9790-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    40002.028 2.067 2.048 2.002
    16001.434 1.457 1.446 1.400
    6000.767 0.785 0.776 0.730
    2000.395 0.413 0.404 0.358
    800.192 0.196 0.194 0.148
    00.045 0.047 0.046 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization.
  • 基因功能参考文献：
    1. FGF-2b and hPL-A are promising candidates for regenerative therapy in Diabetes Mellitus by inducing de-differentiation of stem cells modulating pivotal endocrine genes. PMID: 29068419
    2. Placental lactogen is expressed but is not translated into protein in breast cancer. PMID: 24475273
    3. These results indicate that placental lactogen induces CYP2E1 expression via PI3-kinase pathway in human hepatocytes. PMID: 24408518
    4. Expression of placental lactogen mRNA is increased in plasma of women with placenta previa and invasive placenta. PMID: 23744883
    5. altered expression of placenta lactogen appears not to be causal for betamethasone-induced fetal growth restriction in the human PMID: 23465880
    6. results are consistent with a pleiotropic effect of placental hGH/CSH genes at the maternal-fetal interface relating to the regulation of fetal growth and the risk of affected maternal metabolism. PMID: 22387044
    7. Data conclude that members of the C/EBP and Ets families can differentially modulate CS-Benh and CS-Aenh activity. PMID: 21737519
    8. hPL-A is involved in the regulation of pancreatic beta cells activity. PMID: 21765243
    9. Results strongly support a conformationally mediated obligate-ordered prolactin receptor binding for each of the three lactogenic hormones: prolactin, growth hormone, and placental lactogen. PMID: 20427283
    10. The human growth hormone/chorionic somatomammotropin at chromosome 17 is implicated in regulation of postnatal and intrauterine growth. PMID: 20233782
    11. Investigation of the GRB2, GRB7, and CSH1 genes as candidates for the Silver-Russell syndrome (SRS) on chromosome 17q. PMID: 11897833
    12. a role for CSH1 haploinsufficiency in the etiology of growth retardation PMID: 14642004
    13. These data implicate ETS-domain family members in the regulation of the CS locus in placenta and pituitary cells. PMID: 14673137
    14. We showed that intra-arterial infusion of human placental lactogen primarily decreased coronary, renal & iliac blood flow. Mechanism of this response was shown to be due to inhibition of vasodilatory beta2-adrenergic receptor-mediated effect. PMID: 16410683
    15. crystal structure of the free state of human PL has been determined to 2.0 A resolution showing the molecule possesses an overall structure similar to other long chain four-helix bundle cytokines PMID: 16546209
    16. The present findings indicate that the concentration of human placental lactogen (hPL) and human chorionic gonadotropin (beta hCG) mRNA is significantly higher in the cellular component of maternal blood samples than in the plasma component. PMID: 16950818
    17. A CSH1 deletion carrier with nearly complete hypomethylation at the ICR1 locus had the characteristic clinical signs of Silver Russel syndrome, ruling out a causal relationship. PMID: 17400796
    18. Placental lactogen is a novel ligand of stabilin-1. PMID: 18292525
    19. Three single nucleotide polymorphisms in CGB5 and CSH1 genes were genotyped for 844 offspring of the cases and controls. Maternal breast cancer risk did not significantly differ by the offspring's carrier status of the three SNPs. PMID: 19047151
    20. maternal smoking associated with a reduction in cord blood hPL PMID: 19290844

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：

    HGNC: 2440

    OMIM: 150200

    KEGG: hsa:1442

    STRING: 9606.ENSP00000316416

    UniGene: Hs.654390