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  4. Human leukocyte immunoglobulin-like receptor subfamily B member 4,LILRB4 ELISA Kit

Human leukocyte immunoglobulin-like receptor subfamily B member 4,LILRB4 ELISA Kit

  • 中文名称：
    人白细胞免疫球蛋白样受体亚家族B成员4(LILRB4)酶联免疫试剂盒
  • 货号：
    CSB-E11812h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human LILRB4 ELISA Kit was designed for the quantitative measurement of Human LILRB4 protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    CD85 antigen-like family member K ELISA Kit; CD85k ELISA Kit; HM18 ELISA Kit; ILT-3 ELISA Kit; ILT3 ELISA Kit; Immunoglobulin-like transcript 3 ELISA Kit; Leukocyte immunoglobulin like receptor subfamily B (with TM and ITIM domains) member 4 ELISA Kit; Leukocyte immunoglobulin-like receptor 5 ELISA Kit; Leukocyte immunoglobulin-like receptor subfamily B member 4 ELISA Kit; LILRB4 ELISA Kit; LILRB5 ELISA Kit; LIR-5 ELISA Kit; LIR5 ELISA Kit; LIRB4_HUMAN ELISA Kit; Monocyte inhibitory receptor HM18 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LILRB4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-98
    1:2Average %97
    Range %93-102
    1:4Average %84
    Range %81-89
    1:8Average %88
    Range %83-95
  • 回收率：
    The recovery of human LILRB4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10198-104
    EDTA plasma (n=4)9289-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.188 2.288 2.238 2.113
    10001.833 1.899 1.866 1.741
    5001.314 1.392 1.353 1.228
    2500.954 0.945 0.950 0.825
    1250.478 0.460 0.469 0.344
    62.50.320 0.344 0.332 0.207
    31.250.211 0.212 0.212 0.087
    00.120 0.129 0.125
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
  • 基因功能参考文献：
    1. Results suggest that ILT3 played an important role in tumor progression in colorectal cancer by possible influence on CD45RO+ T cells in the tumor microenvironment. PMID: 30126665
    2. These results suggest that tyrosine phosphorylation may be critical in FcgammaRI-dependent endocytosis/phagocytosis that may be regulated by LILRB4 by triggering dephosphorylation of key signalling proteins. PMID: 27725776
    3. ILT3 may functionally contribute to a regulatory network controlling tumor progression by suppressing the Akt pathway. PMID: 28931525
    4. The ILT3 PBs/PCs were suggested to be developmentally equivalent based on the simultaneous generation of these populations upon activation of memory B cells in vitro ILT3 expression was found to be induced efficiently by IL-2, while IFN-alpha effectively induced ILT3 PBs/PCs in vitro Utilizing the elevated ILT3 will support opening a new avenue for molecular markers for, pathogenic cells. PMID: 27742834
    5. this study shows that LILRB4 might have dual inhibitory and activating functions, depending on the position of the functional tyrosine residues in its immunoreceptor tyrosine-based inhibitory motifs and/or the nature of the stimuli PMID: 28409541
    6. LILRB4 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    7. Identification of ILT4 as a cellular receptor for CSP C4d PMID: 26678451
    8. ILT3Fc inhibits T cell activation and induces the generation of suppressor T cells targeting multiple inflammatory miRNA pathways. PMID: 22387553
    9. Crystal structure of leukocyte Ig-like receptor LILRB4 (ILT3/LIR-5/CD85k): a myeloid inhibitory receptor involved in immune tolerance. PMID: 21454581
    10. Data show that LILRB4 is a potent inhibitor of monocyte activation via FcgammaRI. PMID: 19833736
    11. ILT3 precursor RNA is expressed and retained in nuclei of resting endothelial cells. PMID: 16433759
    12. Our findings indicate that expression of ILT3 and ILT4 on CLL B cells represents a phenotypic abnormality that may play a role in tolerization of tumor-specific T cells. PMID: 17266150
    13. The inhibitory effect of serum and membrane ILT3 in a humanized SCID mouse model describes an immune-escape mechanism that could contribute to impaired T cell responses in patients with cancer. PMID: 17513794
    14. Both membrane and soluble ILT3 are proteins with potent immunosuppressive activity which are of importance for treatment of rejection, autoimmunity and cancer. PMID: 17923119
    15. describe an immune-escape mechanism mediated by the inhibitory receptor immunoglobulin-like transcript 3 (ILT3) which may be responsible for failure of pancreatic cancer therapy PMID: 17993722
    16. Progenitor mast cells expressed cell surface inhibitory LILRB4. Mature cord-blood-derived mast cells had detectable mRNA encoding multiple LILRs, none were expressed on the cell surface. PMID: 17998301
    17. ILT3-Fc is a potent immunoregulatory agent that suppresses islet allograft rejection in humanized NOD/SCID mice. PMID: 18420485
    18. 15 single nucleotide polymorphisms are identified in the extracellular domain of immunoglobulin-like transcript 3 gene from healthy individuals. PMID: 18486764
    19. Expressed on cultured osteoclast precursor cells derived from peripheral blood monocytes; could be inhibitory for osteoclast development in presence of receptor activator of NF-kappaB ligand (RANKL) and macrophage colony stimulating factor (M-CSF). PMID: 18802077
    20. A significant association is observed between ILT3 dendritic cells in kidney transplants and donor age. PMID: 19010139
    21. ILT3 may play a critical role in the control of inflammation. PMID: 19380766
    22. A tryptophan-deprived environment generates monocyte-derived dendritic cells with a marked up-regulation of inhibitory receptors ILT3 and ILT4 and enhanced capacity to induce CD4+CD25+Foxp3+ regulatory T cells in an ILT3-dependent manner. PMID: 19535644

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Note=Ligand binding leads to internalization and translocation to an antigen-processing compartment.
  • 组织特异性：
    Detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells.
  • 数据库链接：

    HGNC: 6608

    OMIM: 604821

    KEGG: hsa:11006

    STRING: 9606.ENSP00000270452

    UniGene: Hs.67846