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  4. Human calcitonin gene related peptide,CGRP ELISA Kit

Human calcitonin gene related peptide,CGRP ELISA Kit

  • 中文名称：
    人降钙素基因相关肽(CGRP)酶联免疫试剂盒
  • 货号：
    CSB-E08210h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CGRP ELISA Kit was designed for the quantitative measurement of Human CGRP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

  • 别名：
    Beta type CGRP ELISA Kit; Beta-CGRP ELISA Kit; Beta-type CGRP ELISA Kit; CALC2 ELISA Kit; Calcb ELISA Kit; CALCB_HUMAN ELISA Kit; Calcitonin gene related peptide 2 ELISA Kit; Calcitonin gene related peptide II ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; Calcitonin related polypeptide; beta ELISA Kit; CGRP II ELISA Kit; CGRP-II ELISA Kit; CGRP2 ELISA Kit; CGRPII ELISA Kit; FLJ30166 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-92
    1:2Average %96
    Range %91-105
    1:4Average %97
    Range %92-110
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of human CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9690-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.660 2.511 2.586 2.432
    502.080 2.152 2.116 1.962
    251.451 1.366 1.409 1.255
    12.50.795 0.744 0.770 0.616
    6.250.491 0.523 0.507 0.353
    3.120.315 0.325 0.320 0.166
    1.560.248 0.244 0.246 0.092
    00.157 0.151 0.154 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

关于人降钙素基因相关肽CGRP ELISA试剂盒（CSB-E08210h），这个CUSABIO的CGRP Sandwich ELISA检测的具体是什么？全长还是片段？这个试验能否检测其他CGRP相关肽？

A:
CSB-E08210h 检测的是Beta-type CGRP，参考https://www.uniprot.org/uniprot/P10092针对全长设计，至于客户提到的other CGRP related peptides理论是检测不了的。如果您有具体信息，可以发送给我们进一步尝试。

靶点详情

  • 功能：
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 基因功能参考文献：
    1. The results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes, and that C12orf39 promoter activity is activated by ATF4 through amino acid response element. PMID: 26967115
    2. Keratinocyte-derived CGRPbeta may modulate epidermal homeostasis through autocrine/paracrine signaling and may contribute to chronic pain under pathological conditions. PMID: 21641113
    3. Calcitonin gene-related peptide stimulates proliferation of alveolar epithelial cells. PMID: 19192276
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 数据库链接：

    HGNC: 1438

    OMIM: 114160

    KEGG: hsa:797

    STRING: 9606.ENSP00000346017

    UniGene: Hs.534305