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  4. Human beta 2 transferrin (β2TF) ELISA kit

Human beta 2 transferrin (β2TF) ELISA kit

  • 中文名称：
    人β2转铁蛋白(β2TF)酶联免疫试剂盒
  • 货号：
    CSB-EQ027323HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    人β2转铁蛋白(β2TF)酶联免疫试剂盒（CSB-EQ027323HU）为双抗夹心法ELISA试剂盒，定量检测血清、cerebrospinalfluid样本中的β2TF含量。试剂盒检测范围为1.25 μg/mL-80 μg/mL，该产品特别适用于神经科学研究领域，可用于探索脑脊液中β2TF水平与血脑屏障功能、神经退行性疾病或颅内感染的相关性，也可用于血清样本中铁代谢异常的机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    beta 2 transferrin, beta2TF
  • 缩写：
    β2TF
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, cerebrospinal fluid
  • 检测范围：
    1.25 μg/mL-80 μg/mL
  • 灵敏度：
    0.31 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human β2TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %87
    Range %83-91
    1:4Average %97
    Range %92-110
    1:8Average %90
    Range %86-94
  • 回收率：
    The recovery of human β2TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10599-114
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µg/mlOD1OD2AverageCorrected
    802.915 2.952 2.934 2.769
    402.210 2.076 2.143 1.978
    201.561 1.604 1.583 1.418
    101.051 1.039 1.045 0.880
    50.616 0.624 0.620 0.455
    2.50.396 0.369 0.383 0.218
    1.250.274 0.279 0.277 0.112
    00.162 0.167 0.165
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

相关问答

■ 常见问题解答
Q:

We intend to start an assay beta 2 transferrin for human CSF detection
We want you to recommend a kit for this assay in clinical samples

A:
Thanks for your inquiry!Regarding the test target,human β2TF,we have the following kit available.We will send you the datasheet as well for reference.
This kit is suitable for CSF samples.Pls take the actual measurement as the real results.And for your information,our elisa kits is only for research purpose,not for diagnosis.
Q:

I am wondering what is the total amount (µg) of biotin conjugated antibody that comes with this kit. Also if you can comment on specificity against regular transferrin.

A:
Thanks for your inquiry! This kit detects beta 2 transferrin. There is biotin-antibody in the kit. The components in the kit are ready to use, so the customers don't need to prepare extra reagents.