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  4. Human Protein FAM132A(FAM132A) ELISA kit

Human Protein FAM132A(FAM132A) ELISA kit

  • 中文名称：
    人蛋白FAM132A(FAM132A)酶联免疫试剂盒
  • 货号：
    CSB-EL008058HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human FAM132A ELISA Kit was designed for the quantitative measurement of Human FAM132A protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

  • 别名：
    C1QTNF12 ELISA Kit; C1QDC2 ELISA Kit; CTRP12 ELISA Kit; FAM132AAdipolin ELISA Kit; Adipose-derived insulin-sensitizing factor ELISA Kit; C1q and TNF related protein 12 ELISA Kit; Complement C1q tumor necrosis factor-related protein 12) [Cleaved into: Adipolin fC1QTNF12 ELISA Kit; Adipolin fCTRP12 ELISA Kit; Adipolin full-length form); Adipolin gC1QTNF12 ELISA Kit; Adipolin cleaved form ELISA Kit; Adipolin gCTRP12)] ELISA Kit
  • 缩写：
    FAM132A
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %85-89
    1:2Average %99
    Range %91-102
    1:4Average %100
    Range %93-108
    1:8Average %94
    Range %90-99
  • 回收率：
    The recovery of human FAM132A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-96
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.380 2.356 2.368 2.237
    10001.815 1.776 1.796 1.665
    5001.220 1.198 1.209 1.078
    2500.669 0.666 0.668 0.537
    1250.438 0.424 0.431 0.300
    62.50.226 0.222 0.224 0.093
    31.250.181 0.178 0.180 0.049
    00.134 0.128 0.131 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Insulin-sensitizing adipocyte-secreted protein (adipokine) that regulates glucose metabolism in liver and adipose tissue. Promotes glucose uptake in adipocytes and suppresses de novo glucose production in hepatocytes via the PI3K-Akt signaling pathway. Administration lead to reduction of blood glucose. Able to attenuate inflammation in fat tissue.
  • 基因功能参考文献：
    1. Adipolin and adiponectin are cytokines that exert substantial impact on obesity, progression of atherosclerosis, insulin resistance, and glucose metabolism. PMID: 29452073
    2. Low serum CTRP12 level is associated with Type 2 diabetes mellitus. PMID: 28207876
    3. Coenzyme Q10 reduced HbA1c considerably in overweight and obese patients with diabetes, although interestingly adipolin levels declined simultaneously. In this study, Q10 modulated glucose homeostasis, which was expected to be mediated by increasing adipolin. The similar mechanisms of action of Q10 and adipolin may justify lowering effect of Q10 on adipolin. PMID: 27657997
    4. Serum adipolin concentrations were significantly lower in polycystic ovarian syndrome compared with control subjects. PMID: 24612181
    5. In subcutaneous adipose tissue explants, insulin significantly increased CTRP12 protein expression and secretion. PMID: 24492466
    6. we describe, for the first time, adipolin expression in human chondrocytes as well as the intracellular mechanism involved in its regulation PMID: 22736092
    7. Endopeptidase cleavage generates a functionally distinct isoform of C1q/tumor necrosis factor-related protein-12 (CTRP12) with an altered oligomeric state and signaling specificity. PMID: 22942287
    8. Reports cloning, expression, regulation and function of mouse adipolin (Fam132a) PMID: 21849507

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  • 亚细胞定位：
    [Adipolin fC1QTNF12]: Secreted.; [Adipolin gC1QTNF12]: Secreted.
  • 蛋白家族：
    Adipolin/erythroferrone family
  • 组织特异性：
    Predominantly expressed by adipose tissues.
  • 数据库链接：

    HGNC: 32308

    OMIM: 616593

    KEGG: hsa:388581

    STRING: 9606.ENSP00000329137

    UniGene: Hs.197613