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  4. Human Phospholipase A1 member A(PLA1A) ELISA kit

Human Phospholipase A1 member A(PLA1A) ELISA kit

  • 中文名称：
    人磷脂酶A1成员A(PLA1A) 酶联免疫试剂盒
  • 货号：
    CSB-EL018084HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    AA986889 ELISA Kit; NMD ELISA Kit; OTTHUMP00000215248 ELISA Kit; OTTHUMP00000215249 ELISA Kit; OTTHUMP00000215250 ELISA Kit; Phosphatidylserine specific phospholipase A1 ELISA Kit; Phosphatidylserine specific phospholipase A1alpha ELISA Kit; Phosphatidylserine-specific phospholipase A1 ELISA Kit; Phospholipase A1 member A ELISA Kit; pla1a ELISA Kit; PLA1A_HUMAN ELISA Kit; PS PLA1 ELISA Kit; PS-PLA1 ELISA Kit; PSPLA 1 ELISA Kit; PSPLA1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLA1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 88    
    Range % 82-95    
    1:2 Average % 97    
    Range % 93-101    
    1:4 Average % 99    
    Range % 95-106    
    1:8 Average % 93    
    Range % 87-99    
  • 回收率：
    The recovery of human PLA1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 91 87-95    
    EDTA plasma (n=4) 99 95-104    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    20 2.340 2.344 2.342 2.237    
    10 1.604 1.741 1.673 1.568    
    5 0.936 0.968 0.952 0.847    
    2.5 0.576 0.554 0.565 0.460    
    1.25 0.353 0.325 0.339 0.234    
    0.625 0.230 0.213 0.222 0.117    
    0.312 0.185 0.180 0.183 0.078    
    0 0.104 0.106 0.105 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 客户评论
  • Sample type: Biological fluids (venom)
    Sample species: Hymenopter
    Review: We only have test mode with 8 wells for samples, we put duplicates of different batches of hymenoptera fluids (2 samples 1:1 and 1:4 dillution). High coeficient of variation between samples, but we have a record and our samples were recogniced by the kit.

靶点详情

  • 功能：
    Hydrolyzes the ester bond of the acyl group attached at the sn-1 position of phosphatidylserines (phospholipase A1 activity) and 1-acyl-2-lysophosphatidylserines (lysophospholipase activity) in the pathway of phosphatidylserines acyl chain remodeling. Cleaves phosphatidylserines exposed on the outer leaflet of the plasma membrane of apoptotic cells producing 2-acyl-1-lysophosphatidylserines, which in turn enhance mast cell activation and histamine production. Has no activity toward other glycerophospholipids including phosphatidylcholines, phosphatidylethanolamines, phosphatidic acids or phosphatidylinositols, or glycerolipids such as triolein.; Hydrolyzes lyso-PS but not PS.
  • 基因功能参考文献：
    1. Serum PS-PLA1 levels were significantly higher in the melanoma subjects and associated with the clinical stages. PMID: 29500864
    2. the LysoPA levels might be determined largely by LysoPC and LysoPI (possible precursors) and the PS-PLA1-mediated pathway might be involved in the production of LysoPS in gastric cancer. PMID: 28143894
    3. PS-PLA1 expression in colorectal cancer is associated with tumor invasion and metastasis. PMID: 25750298
    4. These data suggest that PLA1A plays an important role in bridging the membrane-associated NS2-E2 complex and the NS5A-associated replication complex via its interaction with hepatitis C virus E2, NS2, and NS5A. PMID: 25505071
    5. PLA1A2 polymorphism is associated with mortality in participants whose hemoglobin A1c ranges from 5.5% to 6.5%. PMID: 24886443
    6. Overall, our study shed the light on new structural features of the phospholipase activity of pancreatic lipase family members. PMID: 24368210
    7. These results suggest that the expression of PS-PLA(1) mRNA in THP-1-derived macrophages is activated via TLR4. PMID: 20573295
    8. polymorphisms in the gene encoding phosphatidylserine-specific phospholipase A1 PMID: 12436198

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 组织特异性：
    Widely expressed. Expressed in placenta, prostate and liver. Weakly or not expressed in skin, leukocytes, platelets, colon, spleen, lung, muscle and kidney.
  • 数据库链接：

    HGNC: 17661

    OMIM: 607460

    KEGG: hsa:51365

    STRING: 9606.ENSP00000273371

    UniGene: Hs.437451