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  4. Human Mitochondrial import inner membrane translocase subunit TIM14(DNAJC19) ELISA kit

Human Mitochondrial import inner membrane translocase subunit TIM14(DNAJC19) ELISA kit

  • 中文名称：
    人线粒体进口内膜转位酶亚基TIM14(DNAJC19)酶联免疫试剂盒
  • 货号：
    CSB-EL007026HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human DNAJC19 ELISA Kit was designed for the quantitative measurement of Human DNAJC19 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL.

  • 别名：
    DnaJ (Hsp40) homolog; subfamily C; member 19 ELISA Kit; DnaJ homolog subfamily C member 19 ELISA Kit; DNAJC19 ELISA Kit; homolog of yeast TIM14 ELISA Kit; Mitochondrial import inner membrane translocase subunit TIM14 ELISA Kit; PAM18 ELISA Kit; TIM 14 ELISA Kit; TIM14 ELISA Kit; TIM14_HUMAN ELISA Kit; TIMM 14 ELISA Kit; TIMM14 ELISA Kit; translocase of the inner mitochondrial membrane 14 ELISA Kit; Translocase of the inner mitochondrial membrane 14; yeast homolog of ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    28 pg/mL-1800 pg/mL
  • 灵敏度：
    7 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DNAJC19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %86
    Range %81-90
    1:4Average %93
    Range %89-98
    1:8Average %90
    Range %85-94
  • 回收率：
    The recovery of human DNAJC19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10399-107
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    18002.656 2.719 2.688 2.579
    9002.287 2.172 2.230 2.121
    4501.653 1.707 1.680 1.571
    2251.135 1.071 1.103 0.994
    112.50.681 0.653 0.667 0.558
    560.374 0.362 0.368 0.259
    280.257 0.252 0.255 0.146
    00.112 0.105 0.109
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Mitochondrial co-chaperone which forms a complex with prohibitins to regulate cardiolipin remodeling. May be a component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity.
  • 基因功能参考文献：
    1. Both DnaJC15 and DnaJC19 formed two distinct subcomplexes with Magmas at the import channel. PMID: 27330077
    2. The loss of DNAJC19/PHB complexes affects cardiolipin acylation and leads to the accumulation of cardiolipin species with altered acyl chains. PMID: 24856930
    3. A report of a new mutation in the human DNAJC19 gene that causes early onset dilated cardiomyopathy syndrome in two brothers of Finnish origin. PMID: 22797137
    4. The association of dilated cardiomyopathy with ataxia (DCMA) syndrome with a segment of 3q26.33 and the identification of a splice mutation in a novel gene DNAJC19 in DCMA patients. PMID: 16055927
    5. Dilated cardiomyopathy caused by homozygous mutations in a novel gene, DNAJC19, presumed to play a role in importation of mitochondrial proteins. PMID: 17244376

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  • 相关疾病：
    3-methylglutaconic aciduria 5 (MGA5)
  • 亚细胞定位：
    Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.
  • 蛋白家族：
    TIM14 family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 30528

    OMIM: 608977

    KEGG: hsa:131118

    STRING: 9606.ENSP00000372005

    UniGene: Hs.230601