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  4. Human Leukocyte cell-derived chemotaxin-2(LECT2) ELISA kit

Human Leukocyte cell-derived chemotaxin-2(LECT2) ELISA kit

  • 中文名称：
    人白细胞衍生趋化因子-2(LECT2)酶联免疫试剂盒
  • 货号：
    CSB-EL012855HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human LECT2 ELISA Kit was designed for the quantitative measurement of Human LECT2 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 别名：
    chm II ELISA Kit; chm2 ELISA Kit; Chondromodulin II ELISA Kit; hLECT2 ELISA Kit; LECT 2 ELISA Kit; LECT-2 ELISA Kit; LECT2 ELISA Kit; LECT2_HUMAN ELISA Kit; Leukocyte cell derived chemotaxin 2 ELISA Kit; Leukocyte cell-derived chemotaxin-2 ELISA Kit; MGC126628 ELISA Kit
  • 缩写：
    LECT2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LECT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %89-97
    1:2Average %98
    Range %95-102
    1:4Average %102
    Range %94-105
    1:8Average %95
    Range %90-99
  • 回收率：
    The recovery of human LECT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-97
    EDTA plasma (n=4)9995-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.902 3.099 3.001 2.809
    502.247 2.392 2.320 2.128
    251.563 1.492 1.528 1.336
    12.50.784 0.771 0.778 0.586
    6.250.423 0.455 0.439 0.247
    3.120.345 0.340 0.343 0.151
    1.560.233 0.253 0.243 0.051
    00.186 0.197 0.192
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your ELISA kit CSB-EL012855HU, could you please advise on the following:
1) Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Does the standard include additive agent such as antiseptic or preservative? If included, what is composition of additive agent?
3) Material name and concentration of Stop Solution.
4) Does this kit include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry!
CSB-EL012855HU
This kit is designed against full length protein.
Capture antibody is rabbit polyclonal antibody and detection antibody is rabbit polyclonal antibody.
Standard is 293 cell-derived recombinant protein.
Preservative is proclin 300(1:3000).
Stop solution is 4N sulfuric acid.
This kit doesn't contain azide, thimerosal, 2-ME.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. Does not show metalloendopeptidase activity.
  • 基因功能参考文献：
    1. Overexpression of LECT2 or treatment with a recombinant LECT2 protein impaired the colony-forming ability and motility of non-small cell lung cancer cells harboring high levels of activated EGFR and MET. PMID: 30453282
    2. Circulating LECT2 concentrations were increased in individuals with NAFLD and those with MetS, but not in those with atherosclerosis. The relationship between LECT2 and both NAFLD and MetS might be mediated by its association with abdominal obesity and lipid metabolism. PMID: 28376109
    3. VFA was the strongest predictor of plasma LECT2 that is a potential biomarker linking visceral obesity to dyslipidemia PMID: 28278265
    4. LECT2 was found to be catalytically inactive as a metalloendopeptidase against various types of peptide sequences, including pentaglycine. PMID: 27334921
    5. the tissue levels of THBS2 and LECT-2 may correlate with the stage of atherosclerosis. PMID: 28039493
    6. findings indicated that serum LECT2 level is negatively associated with the presence of diabetic retinopathy and suggest that low circulating LECT2 level is a risk factor for diabetic retinopathy PMID: 27816666
    7. LECT2 amyloidosis is prevalent among Hispanic decedents in New Mexico. PMID: 26912093
    8. ALECT2, which was initially thought to affect mainly Hispanics in the United States, appears to represent an important and likely underrecognized etiology of chronic kidney disease among Egyptians and probably in other ethnic groups around the world PMID: 26867784
    9. Localized LECT2 amyloidosis of the adrenal gland with coexisting MGUS: a diagnostic challenge. PMID: 26048244
    10. LECT2 immunostain is useful in confirming subtype of amyloid within the liver in ALECT2.. PMID: 25602789
    11. Amyloidosis derived from leukocyte chemotactic factor 2 (ALECT2) is a recently described disease. Here, we report the characteristics and outcome of 72 patients with renal ALECT2, which included 19 who had another kidney disease on biopsy. PMID: 24451324
    12. There were no mutations detected in the LECT2 gene, although all renal leukocyte chemotactic factor 2 amyloidosis patients tested were homozygous for the G nucleotide in a non-synonymous SNP at position 172. PMID: 24522497
    13. LECT2 is regulated by beta-catenin in HCC in both mice and men, but serum LECT2 reflects beta-catenin activity only in mice. Serum LECT2 could be a potential biomarker of HCC in patients. PMID: 24892551
    14. First Nations people from British Columbia who presented with chronic kidney disease, were found to be due to LECT2 amyloidosis. PMID: 25064673
    15. The study indicates that serum LECT2 levels are increased by obesity and fatty liver, and suggests that LECT2 is a novel obesity-related protein. PMID: 24390366
    16. The data from this study show that weak LECT2 staining should be regarded as indeterminate or a negative result and does not per se allow diagnosis of specific amyloid type. The diagnosis of LECT2 renal amyloidosis may require LMD/MS confirmation. PMID: 24792621
    17. These results demonstrate the involvement of LECT2 in glucose metabolism and suggest that LECT2 may be a therapeutic target for obesity-associated insulin resistance. PMID: 24478397
    18. ALect2 is a common cause of hepatic amyloidosis in the population of the United States, and subtyping hepatic amyloid deposits by an accurate analytic method such as MS is required for optimal clinical management of hepatic amyloidosis patients PMID: 24415538
    19. These findings reveal a novel, specific inhibitory function of LECT2 in hepatocellular carcinoma by the direct binding and inactivation of MET. PMID: 24114941
    20. preliminary X-ray analysis of human leukocyte cell-derived chemotaxin 2 (LECT2). PMID: 23519812
    21. Re-expression of LECT2 significantly reduced the migration and invasion of human hepatocellular carcinoma cells in vitro and significantly reduced their growth in vivo. PMID: 21394108
    22. LECT2-associated renal amyloidosis represents a unique and perhaps not uncommon disease, especially in Mexican Americans. PMID: 20951486
    23. LECT2, which encodes a protein with chemotactic properties for human neutrophils, is a direct target gene of Wnt/beta-catenin signaling in the liver. PMID: 15239100
    24. LECT2 participates in liver regeneration and injury following hepatectomy. PMID: 15561248
    25. Serum LECT2 levels may be a prognostic indicator of recovery from liver failure. PMID: 15561249

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  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    LECT2/MIM-1 family
  • 组织特异性：
    Highly expressed in adult and fetal liver and weakly in testis. Not expressed in bone marrow.
  • 数据库链接：

    HGNC: 6550

    OMIM: 602882

    KEGG: hsa:3950

    STRING: 9606.ENSP00000274507

    UniGene: Hs.512580