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  4. Human Klotho ELISA Kit

Human Klotho ELISA Kit

  • 中文名称：
    人Klotho酶联免疫试剂盒
  • 货号：
    CSB-E13235h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        KL（Klotho）是一种跨膜蛋白，最初在老鼠的基因研究中发现，对寿命具有显著的调节作用。KL主要分布在肾小管和脑部神经元中，是一种重要的生长因子和信号分子，参与调节细胞代谢、能量代谢、细胞凋亡等生物学过程。KL还与多种疾病的发生和发展相关，包括糖尿病、肾脏疾病、神经系统疾病等。
        j9九游会登录入口首页生物所提供的Human Klotho ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的KL，其灵敏度为0.039 ng/mL，检测范围为0.156 ng/mL-10 ng/mL。
     
  • 别名：
    Kl ELISA Kit; KLOT_HUMAN ELISA Kit; Klotho peptide ELISA Kit
  • 缩写：
    KL
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Developmental Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 86-92  
    1:2 Average % 98  
    Range % 91-105  
    1:4 Average % 96  
    Range % 92-102  
    1:8 Average % 93  
    Range % 86-99  
  • 回收率：
    The recovery of human Klotho spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 97 90-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.491 2.452 2.472 2.350  
    5 2.104 2.076 2.090 1.968  
    2.5 1.504 1.562 1.533 1.411  
    1.25 0.869 0.931 0.900 0.778  
    0.625 0.494 0.507 0.501 0.379  
    0.312 0.292 0.279 0.286 0.164  
    0.156 0.185 0.179 0.182 0.060  
    0 0.126 0.117 0.122 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human klotho antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled klotho antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

Does the kit come with the QCs included? Would it be possible to have a control sample for this kit? Sorry for the confusion, that’s what they meant for QC

A:
Thanks for your inquiry.
The standard of this kit plays the role as quality control.
So sorry that there is no separate quality control sample to provide.

靶点详情

  • 最新研究进展：
    KL是一种与免疫系统相关的细胞因子，其全称为Klotho。KL最初被发现与衰老和老年疾病相关，但最近的研究表明KL对免疫反应、代谢和器官发育等方面也有重要作用。例如，研究人员发现KL可以抑制免疫细胞的激活和炎症反应，并且可以减轻一些自身免疫性疾病的症状。此外，KL还参与了一些代谢性疾病的发生和发展，如糖尿病和动脉粥样硬化等。
  • 功能：
    May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.; The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
  • 基因功能参考文献：
    1. sKlotho is a biomarker of renal dysfunction and a potential treatment target for renoprotection in T2 diabetes mellitus. PMID: 29509906
    2. Soluble klotho levels were inversely related to brain atrophy in alcoholic patients. PMID: 29846497
    3. The tissue klotho protein expression was associated with a lower risk and progression of malignancies. Klotho may be a protective factor against malignancies risk/progression. PMID: 29775618
    4. Serum Klotho may be a novel risk factor for atherosclerosis in maintenance hemodialysis patients PMID: 30064142
    5. a single maximal aerobic exercise session of 20 min duration induced serum levels of klotho, particularly in women PMID: 27251899
    6. Knockdown of klotho gene expression normalized IGF-1R and Wnt1 protein expressions and Akt phosphorylation in granulosa cells from patients with PCOS; it also blocked the effects of insulin on apoptosis and proliferation in granulosa cells. PMID: 28673204
    7. Schizophrenia patients, KL-VS has a selective effect on memory, with heterozygotes in CD and CS clusters performing worse than non-carriers PMID: 28673754
    8. The change in concentration of soluble alpha-klotho during the 1.5-years follow-up was an indicator of CKD progression. Renal damage associated with a reduction of alpha-klotho may involve the upregulation of plasma aldosterone. PMID: 29843135
    9. Cord blood klotho levels were inversely correlated with leptin and insulin levels at birth PMID: 29648999
    10. solubility-Klotho level was closely correlated with kidney function PMID: 29061648
    11. To evaluate the role of klotho in the pathogenesis of systemic sclerosis, its serum concentration was measured in patients compared to healthy controls, and its association with cutaneous and visceral involvement was assessed. Spearman's test showed no significant association between klotho serum levels and systemic sclerosis activity, concerning either clinical, laboratory or instrumental findings. PMID: 29320841
    12. Klotho may play an inhibiting role in lipopolysaccharide-induced inflammation injury by inhibiting NF-kappaB and Wnt signaling pathways in HK-2 cells. PMID: 29441993
    13. High klotho expression is associated with renal fibrosis and podocyte injuries. PMID: 29590173
    14. Although our study revealed higher serum ET-1 and lower serum alpha-Klotho levels in systemic sclerosis patients compared to healthy controls, there were not any significant correlations between their serum levels with severity of organ involvement. PMID: 28831601
    15. The A allele of the G395A polymorphism of Klotho, which emerges the higher levels of phosphate, may be associated with the risk of mortality in Iranian patients receiving hemodialysis. PMID: 29190606
    16. This study showed that A reduced blood alpha-Klotho level was correlated with grading of cerebral Deep White Matter Lesions and was accompanied by cognitive decline as an independent risk factor. PMID: 29154273
    17. Klotho deficiency is not just a biomarker but a contributing factor to the development of acute kidney injury. PMID: 29550818
    18. Forced expression of Klotho resulted in decline of activation of IGF-1R signaling, accompanied by decreased phosphorylation of its downstream targets, including AKT and ERK1/2. These data indicated that Klotho acts as a tumor suppressor via inhibiting IGF-1R signaling, thus suppressing the viability and promoting apoptosis in Tcell lymphoma. PMID: 28656297
    19. serum levels increase following kidney transplantation, although changes are modest compared to more significant improvements in renal function and iFGF-23 levels PMID: 28120476
    20. The functional KL-VS variant of the KLOTHO gene protects against the development of retinopathy in patients with T1 diabetes. PMID: 29247834
    21. the immunohistochemical expressions of Klotho and DNMT3a in tissues obtained from oral dysplasia and oral squamous cell carcinoma, is reported. PMID: 28303350
    22. Klotho suppresses Sox9 upregulation and intranuclear translocation. Klotho inhibits high phosphate-induced osteogenic activity in human aortic valve interstitial cells. PMID: 28332126
    23. Data suggest that serum levels of alpha-klotho and beta-klotho are down-regulated in patients with type 2 diabetes, regardless of diabetic complications. PMID: 27916483
    24. Low expression levels of Klotho were observed in DLBCL patients and cell lines. Enforced expression of Klotho could significantly induce cell apoptosis and inhibit tumor growth in DLBCL. Upregulation of Klotho resulted in declined activation of IGF-1R signaling, accompanied with decreased phosphorylation of its downstream targets, including AKT and ERK1/2. PMID: 28153033
    25. Data suggest that plasma alpha-klotho is down-regulated in subjects with type 2 diabetes as diabetic nephropathy progresses; plasma alpha-klotho level (not urinary alpha-klotho level) may be an early biomarker for predicting renal impairment in type 2 diabetes. PMID: 27037042
    26. Hmgb1 can inhibit Klotho gene expression and malignant phenotype in melanoma cells through activation of NF-kappaB signaling. PMID: 27779100
    27. patients with autosomal dominant polycystic kidney disease may potentially have different soluble alpha-Klotho production/clearance characteristics than the patients with other parenchymal renal diseases. PMID: 27733443
    28. KL and SFRP1 methylation were more predominant in nasopharyngeal tumors. PMID: 28812223
    29. The beta-Klotho gene expresses only in retina and optic nerve. The gamma-Klotho gene may regulate human lens epithelial cells' function mainly by the MAPK/ERK1/2 signaling pathway. PMID: 28095050
    30. Down-Regulation of Soluble alpha-Klotho is Associated with Reduction in Serum Irisin Levels in Chronic Obstructive Pulmonary Disease. soluble a-klotho is one possible factor involved in reduced irisin release from skeletal muscle PMID: 27140192
    31. Klotho is a co-receptor for FGF23. Together they play an important role in bone mineral and vitamin D metabolism. In chronic kidney disease, disturbances in bone metabolism increase cardiovascular risk. In the kidney, klotho induces phosphaturia. Klotho levels drop in chronic kidney disease. It inhibits vascular calcification. Review. PMID: 27118192
    32. A single copy of klotho allele KL-VS is associated with longevity, better health, increased cognition, and bigger regional brain volume. PMID: 28431289
    33. Review of Klotho function and dysfunction in mice and humans. [Review Article] PMID: 27576165
    34. The Klotho KL-VS variant is associated with an increase in the incidence of dementia in older men, in a dose-dependent fashion (intermediate for heterozygosis and highest for homozygosis). PMID: 28539162
    35. Our findings provide the first evidence that alpha-Klotho is significantly decreased in the microvasculature in systemic sclerosis skin PMID: 28183357
    36. indicates an inverse interrelationship between inflammation and Klotho in atherosclerosis PMID: 28963437
    37. Administration of soluble klotho significantly reduced obstruction-induced renal fibrosis in wild-type mice, but not in Trpc6 knockout mice, indicating that klotho and TRPC6 inhibition act in the same pathway to protect against obstruction-induced renal fibrosis. PMID: 27979597
    38. The role of Klotho protein in aging and central nervous system pathology is reviewed. PMID: 28988528
    39. These results support the notion that sialogangliosides and lipid rafts are membrane receptors for sKlotho and that the KL1 domain is sufficient for the tested biologic activities. PMID: 28442546
    40. Klotho is expressed in human dermal fibroblasts and dermal microvascular endothelial cells and is implicated in the process of angiogenesis. PMID: 27260080
    41. A factor for functioning of the brain in aged is KLOTHO gene. PMID: 26405063
    42. Data suggest Klotho attenuates ox-LDL- (oxidized low density lipoprotein)-induced oxidative stress in vascular endothelial cells via up-regulation of oxidative scavengers (lipoprotein and nitric oxide), activation of the PI3K/Akt/eNOS signaling, and down-regulation of LOX1 (lectin-like ox-LDL receptor) expression. (PI3K = phosphatidylinositol 3-Kinase; Akt = proto-oncogene c-Akt; eNOS = endothelial nitric oxide synthase) PMID: 28407763
    43. Targeting klotho may thus represent a therapeutic strategy . PMID: 27696667
    44. FGF23 affected NO synthesis and oxidative stress in human coronary artery endothelial cells are modulated by Klotho. PMID: 27448998
    45. Conversely, siRNA-mediated Klotho silencing up-regulated Egr-1, FN, and Col I expression and the p-Smad3/Smad3 ratio. Moreover, the effects of si-Klotho on Egr-1 expression were abolished by the TGF-beta1 inhibitor SB-431542. Klotho overexpression can prevent mesangial ECM production in high-glucose-treated human MCs, an effect that has been partially attributed to Egr-1 down-regulation facilitated by TGF-beta1/Smad3 s... PMID: 28411025
    46. Klotho levels were higher in acute kidney injury patients and females and lower in end stage renal disease patients than in healthy adults and patients with moderate chronic kidney disease. PMID: 28157067
    47. Genetic polymorphisms such as the G-395A polymorphism in the promoter region of the Klotho gene have been associated with the development of essential hypertension. PMID: 27215557
    48. Klotho was demonstrated to act as a potent tumor suppressor in human ovarian cancer cells. Reduced Klotho expression was detected in the specimens of patients with ovarian cancer, and overexpression of Klotho significantly inhibited cell proliferation of human ovarian cancer cells. PMID: 28259911
    49. sKlotho is increased in active acromegaly and normalises after successful treatment. PMID: 27387242
    50. Any polymorphism altering the function of klotho gene may result with stone formation. We found that there are more GG sequences of G395A gene in patients with urinary tract stone disease. That may be a polymorphism of klotho gene which results with stone formation. PMID: 26597587

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  • 相关疾病：
    Tumoral calcinosis, hyperphosphatemic, familial (HFTC)
  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Apical cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Klotho peptide]: Secreted.
  • 蛋白家族：
    Glycosyl hydrolase 1 family, Klotho subfamily
  • 组织特异性：
    Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chro
  • 数据库链接：

    HGNC: 6344

    OMIM: 211900

    KEGG: hsa:9365

    STRING: 9606.ENSP00000369442

    UniGene: Hs.524953