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  4. Human Kallikrein-5(KLK5) ELISA kit

Human Kallikrein-5(KLK5) ELISA kit

  • 中文名称：
    人激肽释放酶-5(KLK5)酶联免疫试剂盒
  • 货号：
    CSB-EL012456HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human KLK5 ELISA Kit was designed for the quantitative measurement of Human KLK5 protein in serum, plasma, cell culture supernates, tissue homogenates, saliva. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    HK5 ELISA Kit; Kallikrein 5 ELISA Kit; Kallikrein L2 ELISA Kit; Kallikrein like 2 ELISA Kit; Kallikrein like protein 2 ELISA Kit; Kallikrein related peptidase 5 ELISA Kit; Kallikrein-5 ELISA Kit; Kallikrein-like protein 2 ELISA Kit; Kallikrein5 ELISA Kit; KLK 5 ELISA Kit; KLK L2 ELISA Kit; KLK-L2 ELISA Kit; KLK5 ELISA Kit; KLK5_HUMAN ELISA Kit; KLKL 2 ELISA Kit; KLKL2 ELISA Kit; SCTE ELISA Kit; Stratum corneum tryptic enzyme ELISA Kit; UNQ570/PRO1132 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, saliva
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human KLK5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %89-98
    1:2Average %98
    Range %93-104
    1:4Average %95
    Range %89-99
    1:8Average %94
    Range %87-98
  • 回收率：
    The recovery of human KLK5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9387-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.624 2.524 2.574 2.461
    20002.096 1.987 2.042 1.929
    10001.381 1.364 1.373 1.260
    5000.719 0.742 0.731 0.618
    2500.409 0.434 0.422 0.309
    1250.297 0.282 0.290 0.177
    62.50.210 0.215 0.213 0.100
    00.112 0.113 0.113
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May be involved in desquamation.
  • 基因功能参考文献：
    1. persistently upregulated KLK5 resulted in atopic dermatitis-like skin architecture and secretion of atopic dermatitis-related cytokines from keratinocytes in a PAR2 independent manner PMID: 28238749
    2. It may be considered that circulating kallikrein 5 is down-regulated in chronic spontaneous urticaria patients, however its potential role and the possible underlying mechanism are unknown. PMID: 28685545
    3. Thus, these findings point to the involvement of KLK5 in the proteolytic activation and spread of seasonal influenza viruses in humans. PMID: 28615200
    4. The results of the present study indicated that the mRNA and protein expression levels of KLK5 were significantly upregulated in CRC tissues and sera, and were associated with an advanced tumor stage. PMID: 27430713
    5. the expression level of hK5 in tumor stromal cells is a promising biomarker for poor prognosis in TNBC. PMID: 26345898
    6. This report demonstrates that concurrent loss of KLK5 and KLK7 associates with a poor clinical outcome in Oral Squamous-Cell Carcinoma and could therefore serve as prognostic marker in this disease. PMID: 26022646
    7. Low KLK5 expression is associated with breast cancer. PMID: 24158494
    8. KLK5 is an important contributor to the Netherton syndrome proteolytic cascade. PMID: 24534191
    9. Data indicate that the KLK5 SNP rs268908 was associated with an increased risk of prostate cancer. PMID: 21741862
    10. Kallikrein 5 and kallikrein 12 cleave human influenza hemagglutinins and activate thrombolytic zymogens. PMID: 23612974
    11. KLK5 is required for biochemical processing of profilaggrin in human skin. PMID: 23629652
    12. the inhibition and binding of different SPINK9 variants towards KLK5, KLK7, KLK8 and KLK14 PMID: 22505519
    13. Loss of KLK5 is associated with ovarian cancer. PMID: 22102857
    14. Increased serum and ascitic fluid KLK5 levels are associated with poor patient outcome, thus underlining the importance of KLK5 as a biomarker for early detection as well as for disease management in ovarian cancer. PMID: 21273346
    15. Significant co-expression of KLKs 5 and 7 was observed in the same cancer samples. Increased KLK5 expression was a statistically significant independent prognostic factor for DFS and OS of patients PMID: 21868565
    16. KLK5 may promote metastatic dissemination of OSCC by promoting loss of junctional integrity through cleavage of desmoglein 1. PMID: 21163944
    17. expression and activity of KLK are under fine control and can be distinctly influenced by variables such as differentiation, calcium, vitamin D, and retinoic acid PMID: 20090765
    18. expression in prostate cancer negatively correlated with cancer aggressiveness PMID: 11948967
    19. We found significantly lower expression of KLK5 in testicular tumors than in normal testicular tissue. PMID: 12385949
    20. High expression of KLK5 transcript with a short 5'-untranslated region is associated with ovarian cancer PMID: 12738725
    21. has trypsin-like substrate specificity and forms complexes with protease inhibitors in serum and ascites fluid from ovarian cancer patients PMID: 12890555
    22. scte induced degradation of three corneodesmosomal components: corneodesmosin, desmocollin 1 and desmoglein 1 PMID: 15140227
    23. may be part of a protease cascade in the stratum corneum, and that the observed pH effects may have physiological relevance PMID: 15654974
    24. hK5 may be implicated in tumor progression, particularly in invasion and angiogenesis PMID: 15713679
    25. variability in KLK5 and KLK7 gene expression might be involved in lung tumorigenesis PMID: 15766562
    26. hK5 is able to internally cleave insulin-like growth factor-binding proteins 1, 2, 3, 4, and 5, but not 6, suggesting that it might be involved in prostate cancer progression through growth factor regulation PMID: 16517595
    27. in majority of patients with Netherton syndrome, Dsg1 & Dsc1 were reduced in living layers of epidermis; SCTE-like & SCCE-like activities were increased, suggesting these proteases participate in premature degradation of corneodesmosomal cadherins PMID: 16628198
    28. KLK5 and KLK7 were shown to control activation of CAP18 and also influence further processing to smaller peptides with alternate biological activity; the balance of proteolytic activity at an epithelial interface will control innate immune defense PMID: 17012259
    29. KLK5 may participate in epidermal desquamation through cleavage of desmoglein 1 and regulation by lympho-epithelial Kazal-type-related inhibitor (LEKTI). PMID: 17158887
    30. A target of gene amplification, which may play a crucial role in promoting cancer-cell invasion in bladder tumor. PMID: 17459052
    31. KLK5 and KLK14, but neither KLK7 nor KLK8, induced PAR2 signalling. PMID: 17625593
    32. Dada shoe that the His96-99-57 triad is responsible for the Zn2+-mediated inhibition of hK5 catalysis. PMID: 17881000
    33. predominant localisation of KLK5 and KLK7 in acinar cells of the exocrine pancreas; KLK5 and KLK7 generate transcripts in pancreas variant from those in skin or ovary PMID: 18163887
    34. KLK4 and KLK5 activate pro-HGFA. PMID: 18221492
    35. the KLK region was subject to copy number imbalances or involved in unbalanced translocations and were associated with increased protein expression of KLKs 5, 6, 7, 8, 9, 10 and 11 in ovarian cancer cells PMID: 19383346
    36. Parallel underexpression of KLK5 and KLK7 mRNA in breast malignancies is reported. PMID: 19453546
    37. KLK5 is a potential new biomarker to be used in combination with other biomarkers for ovarian cancer detection. PMID: 19820362

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Kallikrein subfamily
  • 组织特异性：
    Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.
  • 数据库链接：

    HGNC: 6366

    OMIM: 605643

    KEGG: hsa:25818

    STRING: 9606.ENSP00000337733

    UniGene: Hs.50915