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  4. Human Growth/differentiation factor 11(GDF11) ELISA kit

Human Growth/differentiation factor 11(GDF11) ELISA kit

  • 中文名称：
    人生长分化因子11(GDF11)酶联免疫试剂盒
  • 货号：
    CSB-EL009344HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human GDF11 ELISA Kit was designed for the quantitative measurement of Human GDF11 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL .

  • 别名：
    BMP 11 ELISA Kit; BMP-11 ELISA Kit; BMP11 ELISA Kit; Bone morphogenetic protein 11 ELISA Kit; GDF 11 ELISA Kit; GDF-11 ELISA Kit; Gdf11 ELISA Kit; GDF11_HUMAN ELISA Kit; Growth differentiation factor 11 ELISA Kit; Growth/differentiation factor 11 ELISA Kit
  • 缩写：
    GDF11
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    28 pg/mL-1800 pg/mL
  • 灵敏度：
    7 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GDF11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %91-99
    1:2Average %88
    Range %81-93
    1:4Average %90
    Range %85-96
    1:8Average %97
    Range %83-91
  • 回收率：
    The recovery of human GDF11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-98
    EDTA plasma (n=4)9489-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    18002.699 2.752 2.726 2.579
    9001.879 1.906 1.893 1.746
    4501.121 1.200 1.161 1.014
    2250.548 0.545 0.547 0.400
    112.50.306 0.332 0.319 0.172
    560.225 0.238 0.232 0.085
    280.213 0.204 0.209 0.062
    00.151 0.142 0.147 　
    　
  • 本试剂盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human GDF11 This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled GDF11 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please provide your reference data for human plasma samples.
e. Did you study the cross-reactivity or interference between Growth Differentiation Factor 11 (GDF11) and analogues? If yes, please provide the corresponding data.

A:
Thanks for your inquiry!
CSB-EL009344HU
Capture antibody is mouse monoclonal antibody and immunogen is CHO cell-derived recombinant protein，full length.
Detection antibody is goat polyclonal antibody and immunogen is CHO cell-derived recombinant protein, fragment. Standard is CHO cell-derived recombinant protein.
We tested undiluted normal serum and plasma samples before and the test value is ND--40pg/ml.
There is no cross-reactivity with human GDF15, human GDF5 and human GDF9.

靶点详情

  • 功能：
    Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues. It is required for proper vertebral patterning and orofacial development. Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3.
  • 基因功能参考文献：
    1. The serum content of GDF11 was much less in esophageal cancer patients than in the control group. Esophageal GDF II in cancer patients was correlated with cancer differentiation: the higher the degree of differentiation, the higher the content of GDF11. PMID: 30213293
    2. Physical inactivity was significantly related to the decreased GDF11 levels in COPD. PMID: 29731621
    3. GDF11 expression was decreased in COPD patients' serum and cells when compared with that of healthy people. PMID: 29680737
    4. GDF11 may be a relevant myostatin-interacting peptide to successful aging in humans PMID: 28701523
    5. The Growth Differentiation Factor 11 (GDF11) and Myostatin (MSTN) in tissue specific aging. PMID: 28472635
    6. Tumor-suppressor inactivation of GDF11 occurs by precursor sequestration in triple-negative breast cancer PMID: 29161592
    7. These studies identify distinctive structural features of GDF11 that enhance its potency, relative to GDF8; however, the biological consequences of these differences remain to be determined. PMID: 28257634
    8. In elderly Chinese women, osteoporosis risk was significantly increased with increases in GDF11 serum levels. PMID: 27557752
    9. A Prodomain Fragment from the Proteolytic Activation of Growth Differentiation Factor 11 Remains Associated with the Mature Growth Factor and Keeps It Soluble PMID: 28715204
    10. MSTN, but not GDF11, declines in healthy men throughout aging. PMID: 27304512
    11. GDF11 is highly concentrated in human platelets. PMID: 27509407
    12. The crystal structure of GDF11 was determined to a resolution of 1.50 A. PMID: 26919518
    13. GDF11 is essential for mammalian development and has been suggested to regulate aging of multiple tissues. It functions in the heart, skeletal muscle, and brain. Review. PMID: 27034275
    14. GDF11 inhibits rather than helps muscle regeneration. PMID: 26001423
    15. Show that there is no age-related cardiac hypertrophy in disease-free 24-month-old C57BL/6 mice and that restoring GDF11 in old mice has no effect on cardiac structure or function. PMID: 26383970
    16. in vitro sprout formation was increased as well by GDF11 treatment PMID: 26026854
    17. Suggest GDF11 functions as encephalic regionalizing factor in neural differentiated mouse embryonic stem cells. PMID: 25352416
    18. GDF11 is a critical rheostat for bone turnover and a key integrator of bone homeostasis. PMID: 25534870
    19. These data demonstrate GDF11 to be a master regulator of neural stem cell transcription that can suppress cell proliferation and migration by regulating the expression of numerous genes involved in both these processes PMID: 24244313
    20. Expression of GDF11, a cytokine which blocks terminal erythroid maturation, was increased in erthyroblasts of thalassemic patients. PMID: 24658077
    21. Quantitative real-time reverse transcription-PCR in colorectal cancer specimens obtained from 130 patients showed that GDF11 mRNA expression in cancer tissue was significantly higher than in normal tissue PMID: 17912435
    22. Members of the transforming growth factor beta (TGFbeta) superfamily, bone morphogenetic protein 2 (BMP2), and growth and differentiation factor 11 (GDF11), can signal cultured RGCs to form dendrites. PMID: 17997109
    23. We propose that Pcsk5, at least in part via GDF11, coordinately regulates caudal Hox paralogs, to control anteroposterior patterning, nephrogenesis, skeletal, and anorectal development. PMID: 18519639
    24. Differential antagonism of activin, myostatin and growth and differentiation factor 11 by wild-type and mutant follistatin. PMID: 18535106
    25. Both WFIKKN1 and WFIKKN2 have high affinity for growth and differentiation factors 8 and 11. PMID: 18596030
    26. Myostatin or 20 ng/mL BMP-11 maintain the colony and cellular morphology of undifferentiated hESC, maintain POU5f1, NANOG, TRA-1-60, and SSEA4 expression, and display increased SMAD2/3 phosphorylation PMID: 19751112

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 组织特异性：
    In the embryo, strong expression is seen in the palatal epithelia, including the medial edge epithelial and midline epithelial seam of the palatal shelves. Less pronounced expression is also seen throughout the palatal shelf and tongue mesenchyme.
  • 数据库链接：

    HGNC: 4216

    OMIM: 603936

    KEGG: hsa:10220

    STRING: 9606.ENSP00000257868

    UniGene: Hs.600883