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  4. Human Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

Human Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

  • 中文名称：
    人XIIA组分泌型磷脂酶A2(PLA2G12A)酶联免疫试剂盒
  • 货号：
    CSB-EL018086HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PLA2G12A ELISA Kit was designed for the quantitative measurement of Human PLA2G12A protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.2 pg/mL.

  • 别名：
    EC 3.1.1.4 ELISA Kit; Group XII secreted phospholipase A2 ELISA Kit; Group XIIA secreted phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 precursor ELISA Kit; GXII ELISA Kit; GXII sPLA2 ELISA Kit; PG12A_HUMAN ELISA Kit; phosphatidylcholine 2 acylhydrolase 12A ELISA Kit; Phosphatidylcholine 2 acylhydrolase GXII ELISA Kit; Phosphatidylcholine 2-acylhydrolase 12A ELISA Kit; Phospholipase A2 group XIIA ELISA Kit; PLA2G12 ELISA Kit; Pla2g12a ELISA Kit; ROSSY ELISA Kit; sPLA2 XII ELISA Kit; sPLA2-XII ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.7 pg/mL-300 pg/mL
  • 灵敏度：
    1.2 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:20 Average % 93    
    Range % 90-96    
    1:40 Average % 107    
    Range % 103-110    
    1:80 Average % 86    
    Range % 83-90    
    1:160 Average % 101    
    Range % 98-106    
  • 回收率：
    The recovery of human PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 85 80-90    
    EDTA plasma (n=4) 101 97-106    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average      
    300 0.236 0.230 0.233      
    150 0.386 0.391 0.389      
    75 0.589 0.592 0.591      
    37.5 0.847 0.834 0.841      
    18.75 1.076 1.100 1.088      
    9.4 1.534 1.502 1.518      
    4.7 1.952 1.991 1.972      
    0 2.345 2.401 2.373      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.
  • 基因功能参考文献：
    1. The study demonstrated that PLA2G12A SNPs or haplotypes might influence the susceptibility to schizophrenia in the Han Chinese population from Northeast China. PMID: 27434078
    2. cellular arachidonate (AA) release and prostaglandin (PG) production are regulated by novel classes of secretory phospholipase A(2)s (sPLA(2)s), groups III and XII PMID: 12522102
  • 亚细胞定位：
    Secreted. Cytoplasm.
  • 蛋白家族：
    Phospholipase A2 family
  • 组织特异性：
    Abundantly expressed in heart, skeletal muscle, kidney, liver and pancreas.
  • 数据库链接：

    HGNC: 18554

    OMIM: 611652

    KEGG: hsa:81579

    STRING: 9606.ENSP00000243501

    UniGene: Hs.389452