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  4. Human Flotillin-1(FLOT1) ELISA kit

Human Flotillin-1(FLOT1) ELISA kit

  • 中文名称：
    人脂筏标记蛋白1(FLOT1)酶联免疫试剂盒
  • 货号：
    CSB-EL008727HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human FLOT1 ELISA Kit was designed for the quantitative measurement of Human FLOT1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

  • 别名：
    FLOT 1 ELISA Kit; FLOT1 ELISA Kit; FLOT1_HUMAN ELISA Kit; Flotillin-1 ELISA Kit; Flotillin1 ELISA Kit; Integral membrane component of caveolae ELISA Kit; Reggie 2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FLOT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-91
    1:2Average %98
    Range %92-102
    1:4Average %95
    Range %88-100
    1:8Average %94
    Range %86-99
  • 回收率：
    The recovery of human FLOT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-100
    EDTA plasma (n=4)9590-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8002.386 2.272 2.329 2.208
    4001.868 1.796 1.832 1.711
    2001.333 1.284 1.309 1.188
    1000.945 0.912 0.929 0.808
    500.600 0.559 0.580 0.459
    250.377 0.369 0.373 0.252
    12.50.256 0.247 0.252 0.131
    00.124 0.118 0.121 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
  • 基因功能参考文献：
    1. flotillin-1 is a tumorigenic protein that plays an important role in promoting the proliferation and tumorigenicity of LUAD. PMID: 28825855
    2. flotillin has a role in desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes PMID: 27346727
    3. Autoantibodies against flotillin-1/2 heterocomplex are present in 1-2% of patients with bona fide multiple sclerosis. PMID: 28645295
    4. Furthermore, we identify flotillin-1 (FLOT1) and histone H1 as downstream factors for cytoplasmic and nuclear pathway of S100A11, which are required for LASP1-S100A11 axis-mediated epithelial-mesenchymal transition and colorectal cancer progression. PMID: 27181092
    5. FLOT1 is associated with aggressive characteristics of HCC PMID: 23840303
    6. This study presents the endocytic pathways of internalization for muscarinic type 3 receptor and flotillin-1/2 in salivary gland epithelial cells. knockdown of flot-1 or -2 by flotillin-specific siRNA prevented internalization and reduced the endocytic efficiency of muscarinic type 3 receptor. PMID: 27221048
    7. Data show that overexpression of 4.1N protein decreased expression of flotillin-1, decreased activation of beta-catenin/Wnt pathway in non-small-cell lung cancer (NSCLC) cells. PMID: 27448302
    8. Upregulation of flotillin-1 promotes invasion and metastasis by activating TGF-beta signaling in nasopharyngeal carcinoma. PMID: 26646322
    9. Flotillin-1 protein is upregulated in human endometrial cancer and localization shifts from epithelial to stromal with increasing tumor grade PMID: 26682635
    10. Expression of flotillin-1 was associated with H-Ras in breast cancer, especially in TNBC (p < 0.001). PMID: 26413934
    11. findings revealed the expression of flotillin 1 in gastric cancer samples is significantly higher than that of noncancer/normal tissues; findings suggest that flotillin 1 may be a novel biomarker for gastric cancer PMID: 25948494
    12. miR-506 expression was down-regulated in clear cell renal cell carcinoma (ccRCC) and correlated with advanced histologic grade, clinical stage, tumor stage, positive lymph node metastasis, and distant metastasis. FLOT1, a potential target gene of miR-506, was inversely correlated with miR-506 expression in ccRCC tissues. PMID: 25793370
    13. polycystins are necessary for assembly of a novel flotillin-containing ciliary signaling complex and provide a molecular rationale for the common renal pathologies caused by OFD1 and polycystin mutations. PMID: 25180832
    14. Findings suggest that FLOT1 plays an important role in the proliferation and recurrence of transitional cell carcinoma. PMID: 24890092
    15. Downregulation of microRNA-182-5p contributes to renal cell carcinoma proliferation via activating the AKT/FOXO3a signaling pathway with FLOT1 being a target. PMID: 24886554
    16. overexpression of FLOT1 can be found in tongue squamous cell cancer patients with higher pathological stage, T classification, N classification or recurrence PMID: 24695539
    17. Flotillin-1 mRNA expression is up-regulated in non-small cell lung cancer patients. PMID: 24533441
    18. a critical role of flotillin-1 in ROS production mediated by c-Src PMID: 24983503
    19. Flotillin-1 regulates oncogenic signaling in neuroblastoma cells by regulating ALK membrane association. PMID: 24830726
    20. The FLOT1 is abundantly expressed in term villous placental CTs and endothelial cells, and in comparison, expression of these proteins in the ST is reduced. PMID: 23064789
    21. Depletion of either flotillin-1 or flotillin-2 resulted in downregulation of ErbB3 and a selective reduction of ErbB2-ErbB3 receptor complexes. PMID: 24747692
    22. study demonstrated that miR-124 might be a tumor suppressor in breast cancer via the regulation of FLOT1 PMID: 24330780
    23. We have identified flotillin 1 and 2 as new partners of the cadherin complexes PMID: 24046456
    24. These data here provide direct evidences for the molecular interaction and endocytosis of PrP(C) with Flot-1 in the presence of copper ions. PMID: 23625312
    25. Patients with higher FLOT1 expression had shorter overall survival time, whereas those with lower FLOT1 expression had longer survival time PMID: 24277378
    26. Human group V secretory phospholipase A2 is associated with lipid rafts and internalized in a flotillindependent pathway. PMID: 24042857
    27. EGFR overexpression and increase in signaling correlates with flotillin-1 expresion. PMID: 24304721
    28. Report role for flotillin 1/2 in interactions of lung epithelial cells with silica nanoparticles. PMID: 22669515
    29. we describe how flotillin-1 and -2 contribute to the stabilization of ErbB2 at the cell surface in breast cancer tissue PMID: 22869152
    30. Flotillin-1 plays an important role in Oral squamous cell carcinoma (OSCC) development. PMID: 23581411
    31. Flotillin-1 interacted with PrPc and promote the endocytosis of PrPc. PMID: 23627023
    32. results support predominant formation of flotillin-1 and -2 hetero-oligomers in resting and chemokine-stimulated human T-cells which may importantly contribute to structuring of the uropod. PMID: 23012365
    33. a novel signaling network containing FRS2, CAP and flotillin-1 PMID: 22235335
    34. receptor-tyrosine kinases may also rely on flotillin-1 upon activation, thus suggesting a general role for flotillin-1 as a novel factor in receptor-tyrosine kinase/MAP kinase signaling. PMID: 22232557
    35. Chlamydia pneumoniae intracellular growth was attenuated in the flotillin-1-silenced cells. PMID: 22215737
    36. FLOT1 plays an important role in promoting proliferation and tumorigenesis of human breast cancer and may represent a novel prognostic biomarker and therapeutic target for the disease. PMID: 21447726
    37. Flot1-enriched membrane microdomains are required for protein kinase C-regulated internalization of the DA transporter (DAT) and the glial glutamate transporter EAAT2. PMID: 21399631
    38. flotillins have a role in NPC1L1-mediated cholesterol uptake and NPC1L1-flotillins-postive cholesterol-enriched membrane microdomains are involved in the mechanism for efficient cholesterol absorption PMID: 21187433
    39. the cellular pathways used by transportan and transportan 10 (TP10) for protein transduction PMID: 19348413
    40. Data show that the BCG phagosome is relatively depleted in LAMP-2, NPC1, flotillin-1, vATPase, and syntaxin 3. PMID: 19815536
    41. In hematopoietic cells, flotillins provide intrinsic cues that govern segregation of certain microdomain-associated molecules during immune cell polarization. PMID: 20027317
    42. Flotillin-1, a marker of rafts, accumulates in lysosomes of neurons in Alzheimer's disease PMID: 14708344
    43. ATP-binding cassette, sub-family A (ABC1), member 1 forms a complex with syntaxin 13 and flotillin-1, residing at the plasma membrane and in phagosomes PMID: 15469992
    44. It is significant that there is an increased gene expression of flotillin-1 in the Parkinson substantia nigra/ventral tegmental area compared to controls. PMID: 15545008
    45. Recombinant flotillin-1 was purified and characterized in in large quantity and high purity. PMID: 15939299
    46. Myocilins with mutations such as G364V, K423E, and Y437H on the domain failed to interact with flotillin-1. PMID: 16198165
    47. Flotillin-1 small interfering RNA (siRNA) inhibited both clathrin-independent uptake of cholera toxin and endocytosis of a GPI-linked protein. We propose that flotillin-1 is one determinant of a clathrin-independent endocytic pathway in mammalian cells. PMID: 16341206
    48. flotillin-1 may recruit amyloid beta (A4) precursor protein to lipid rafts and therefore participate in the localization and processing of APP PMID: 16480949
    49. The results showed that flotillin-1 can interact with arginase1, and hence arginase activity was up-regulated by 26.8%. PMID: 17113085
    50. These lines of evidence suggested that a Gq-coupled receptor activates specifically p38 MAPK through lipid rafts and Src kinase activation, in which flotillins positively modulate the Gq signaling. PMID: 17307333

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Endosome. Membrane, caveola; Peripheral membrane protein. Melanosome. Membrane raft.
  • 蛋白家族：
    Band 7/mec-2 family, Flotillin subfamily
  • 数据库链接：

    HGNC: 3757

    OMIM: 606998

    KEGG: hsa:10211

    STRING: 9606.ENSP00000365569

    UniGene: Hs.179986