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  4. Recombinant Human Flotillin-1 (FLOT1)

Recombinant Human Flotillin-1 (FLOT1)

  • 中文名称：
    人FLOT1重组蛋白
  • 货号：
    CSB-YP008727HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人FLOT1重组蛋白
  • 货号：
    CSB-EP008727HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人FLOT1重组蛋白
  • 货号：
    CSB-EP008727HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人FLOT1重组蛋白
  • 货号：
    CSB-BP008727HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人FLOT1重组蛋白
  • 货号：
    CSB-MP008727HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    FLOT 1; FLOT1; FLOT1_HUMAN; Flotillin-1; Flotillin1; Integral membrane component of caveolae; Reggie 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-427
  • 氨基酸序列
    MFFTCGPNEA MVVSGFCRSP PVMVAGGRVF VLPCIQQIQR ISLNTLTLNV KSEKVYTRHG VPISVTGIAQ VKIQGQNKEM LAAACQMFLG KTEAEIAHIA LETLEGHQRA IMAHMTVEEI YKDRQKFSEQ VFKVASSDLV NMGISVVSYT LKDIHDDQDY LHSLGKARTA QVQKDARIGE AEAKRDAGIR EAKAKQEKVS AQYLSEIEMA KAQRDYELKK AAYDIEVNTR RAQADLAYQL QVAKTKQQIE EQRVQVQVVE RAQQVAVQEQ EIARREKELE ARVRKPAEAE RYKLERLAEA EKSQLIMQAE AEAASVRMRG EAEAFAIGAR ARAEAEQMAK KAEAFQLYQE AAQLDMLLEK LPQVAEEISG PLTSANKITL VSSGSGTMGA AKVTGEVLDI LTRLPESVER LTGVSISQVN HKPLRTA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
  • 基因功能参考文献：
    1. flotillin-1 is a tumorigenic protein that plays an important role in promoting the proliferation and tumorigenicity of LUAD. PMID: 28825855
    2. flotillin has a role in desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes PMID: 27346727
    3. Autoantibodies against flotillin-1/2 heterocomplex are present in 1-2% of patients with bona fide multiple sclerosis. PMID: 28645295
    4. Furthermore, we identify flotillin-1 (FLOT1) and histone H1 as downstream factors for cytoplasmic and nuclear pathway of S100A11, which are required for LASP1-S100A11 axis-mediated epithelial-mesenchymal transition and colorectal cancer progression. PMID: 27181092
    5. FLOT1 is associated with aggressive characteristics of HCC PMID: 23840303
    6. This study presents the endocytic pathways of internalization for muscarinic type 3 receptor and flotillin-1/2 in salivary gland epithelial cells. knockdown of flot-1 or -2 by flotillin-specific siRNA prevented internalization and reduced the endocytic efficiency of muscarinic type 3 receptor. PMID: 27221048
    7. Data show that overexpression of 4.1N protein decreased expression of flotillin-1, decreased activation of beta-catenin/Wnt pathway in non-small-cell lung cancer (NSCLC) cells. PMID: 27448302
    8. Upregulation of flotillin-1 promotes invasion and metastasis by activating TGF-beta signaling in nasopharyngeal carcinoma. PMID: 26646322
    9. Flotillin-1 protein is upregulated in human endometrial cancer and localization shifts from epithelial to stromal with increasing tumor grade PMID: 26682635
    10. Expression of flotillin-1 was associated with H-Ras in breast cancer, especially in TNBC (p < 0.001). PMID: 26413934
    11. findings revealed the expression of flotillin 1 in gastric cancer samples is significantly higher than that of noncancer/normal tissues; findings suggest that flotillin 1 may be a novel biomarker for gastric cancer PMID: 25948494
    12. miR-506 expression was down-regulated in clear cell renal cell carcinoma (ccRCC) and correlated with advanced histologic grade, clinical stage, tumor stage, positive lymph node metastasis, and distant metastasis. FLOT1, a potential target gene of miR-506, was inversely correlated with miR-506 expression in ccRCC tissues. PMID: 25793370
    13. polycystins are necessary for assembly of a novel flotillin-containing ciliary signaling complex and provide a molecular rationale for the common renal pathologies caused by OFD1 and polycystin mutations. PMID: 25180832
    14. Findings suggest that FLOT1 plays an important role in the proliferation and recurrence of transitional cell carcinoma. PMID: 24890092
    15. Downregulation of microRNA-182-5p contributes to renal cell carcinoma proliferation via activating the AKT/FOXO3a signaling pathway with FLOT1 being a target. PMID: 24886554
    16. overexpression of FLOT1 can be found in tongue squamous cell cancer patients with higher pathological stage, T classification, N classification or recurrence PMID: 24695539
    17. Flotillin-1 mRNA expression is up-regulated in non-small cell lung cancer patients. PMID: 24533441
    18. a critical role of flotillin-1 in ROS production mediated by c-Src PMID: 24983503
    19. Flotillin-1 regulates oncogenic signaling in neuroblastoma cells by regulating ALK membrane association. PMID: 24830726
    20. The FLOT1 is abundantly expressed in term villous placental CTs and endothelial cells, and in comparison, expression of these proteins in the ST is reduced. PMID: 23064789
    21. Depletion of either flotillin-1 or flotillin-2 resulted in downregulation of ErbB3 and a selective reduction of ErbB2-ErbB3 receptor complexes. PMID: 24747692
    22. study demonstrated that miR-124 might be a tumor suppressor in breast cancer via the regulation of FLOT1 PMID: 24330780
    23. We have identified flotillin 1 and 2 as new partners of the cadherin complexes PMID: 24046456
    24. These data here provide direct evidences for the molecular interaction and endocytosis of PrP(C) with Flot-1 in the presence of copper ions. PMID: 23625312
    25. Patients with higher FLOT1 expression had shorter overall survival time, whereas those with lower FLOT1 expression had longer survival time PMID: 24277378
    26. Human group V secretory phospholipase A2 is associated with lipid rafts and internalized in a flotillindependent pathway. PMID: 24042857
    27. EGFR overexpression and increase in signaling correlates with flotillin-1 expresion. PMID: 24304721
    28. Report role for flotillin 1/2 in interactions of lung epithelial cells with silica nanoparticles. PMID: 22669515
    29. we describe how flotillin-1 and -2 contribute to the stabilization of ErbB2 at the cell surface in breast cancer tissue PMID: 22869152
    30. Flotillin-1 plays an important role in Oral squamous cell carcinoma (OSCC) development. PMID: 23581411
    31. Flotillin-1 interacted with PrPc and promote the endocytosis of PrPc. PMID: 23627023
    32. results support predominant formation of flotillin-1 and -2 hetero-oligomers in resting and chemokine-stimulated human T-cells which may importantly contribute to structuring of the uropod. PMID: 23012365
    33. a novel signaling network containing FRS2, CAP and flotillin-1 PMID: 22235335
    34. receptor-tyrosine kinases may also rely on flotillin-1 upon activation, thus suggesting a general role for flotillin-1 as a novel factor in receptor-tyrosine kinase/MAP kinase signaling. PMID: 22232557
    35. Chlamydia pneumoniae intracellular growth was attenuated in the flotillin-1-silenced cells. PMID: 22215737
    36. FLOT1 plays an important role in promoting proliferation and tumorigenesis of human breast cancer and may represent a novel prognostic biomarker and therapeutic target for the disease. PMID: 21447726
    37. Flot1-enriched membrane microdomains are required for protein kinase C-regulated internalization of the DA transporter (DAT) and the glial glutamate transporter EAAT2. PMID: 21399631
    38. flotillins have a role in NPC1L1-mediated cholesterol uptake and NPC1L1-flotillins-postive cholesterol-enriched membrane microdomains are involved in the mechanism for efficient cholesterol absorption PMID: 21187433
    39. the cellular pathways used by transportan and transportan 10 (TP10) for protein transduction PMID: 19348413
    40. Data show that the BCG phagosome is relatively depleted in LAMP-2, NPC1, flotillin-1, vATPase, and syntaxin 3. PMID: 19815536
    41. In hematopoietic cells, flotillins provide intrinsic cues that govern segregation of certain microdomain-associated molecules during immune cell polarization. PMID: 20027317
    42. Flotillin-1, a marker of rafts, accumulates in lysosomes of neurons in Alzheimer's disease PMID: 14708344
    43. ATP-binding cassette, sub-family A (ABC1), member 1 forms a complex with syntaxin 13 and flotillin-1, residing at the plasma membrane and in phagosomes PMID: 15469992
    44. It is significant that there is an increased gene expression of flotillin-1 in the Parkinson substantia nigra/ventral tegmental area compared to controls. PMID: 15545008
    45. Recombinant flotillin-1 was purified and characterized in in large quantity and high purity. PMID: 15939299
    46. Myocilins with mutations such as G364V, K423E, and Y437H on the domain failed to interact with flotillin-1. PMID: 16198165
    47. Flotillin-1 small interfering RNA (siRNA) inhibited both clathrin-independent uptake of cholera toxin and endocytosis of a GPI-linked protein. We propose that flotillin-1 is one determinant of a clathrin-independent endocytic pathway in mammalian cells. PMID: 16341206
    48. flotillin-1 may recruit amyloid beta (A4) precursor protein to lipid rafts and therefore participate in the localization and processing of APP PMID: 16480949
    49. The results showed that flotillin-1 can interact with arginase1, and hence arginase activity was up-regulated by 26.8%. PMID: 17113085
    50. These lines of evidence suggested that a Gq-coupled receptor activates specifically p38 MAPK through lipid rafts and Src kinase activation, in which flotillins positively modulate the Gq signaling. PMID: 17307333

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Endosome. Membrane, caveola; Peripheral membrane protein. Melanosome. Membrane raft.
  • 蛋白家族：
    Band 7/mec-2 family, Flotillin subfamily
  • 数据库链接：

    HGNC: 3757

    OMIM: 606998

    KEGG: hsa:10211

    STRING: 9606.ENSP00000365569

    UniGene: Hs.179986