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Human Collectin-12(COLEC12) ELISA kit

  • 中文名称：
    人胶原凝集素-12(COLEC12)酶联免疫试剂盒
  • 货号：
    CSB-EL005762HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human COLEC12 ELISA Kit was designed for the quantitative measurement of Human COLEC12 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    CL P1 ELISA Kit; CL-P1 ELISA Kit; CLP1 ELISA Kit; COL12_HUMAN ELISA Kit; COLEC 12 ELISA Kit; colec12 ELISA Kit; Collectin placenta 1 ELISA Kit; Collectin placenta protein 1 ELISA Kit; Collectin sub family member 12 ELISA Kit; Collectin-12 ELISA Kit; hCL P1 ELISA Kit; hCL-P1 ELISA Kit; NSR2 ELISA Kit; Nurse cell scavenger receptor 2 ELISA Kit; SCARA4 ELISA Kit; Scavenger receptor class A member 4 ELISA Kit; Scavenger receptor with C type lectin ELISA Kit; Scavenger receptor with C-type lectin ELISA Kit; SRCL ELISA Kit
  • 缩写：
    COLEC12
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-88
    1:2 Average % 85
    Range % 82-89
    1:4 Average % 91
    Range % 86-95
    1:8 Average % 104
    Range % 101-107
  • 回收率：
    The recovery of human COLEC12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 85-93
    EDTA plasma (n=4) 90 85-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.541 2.698 2.620 2.498
    10 2.039 2.000 2.020 1.898
    5 1.307 1.368 1.338 1.216
    2.5 0.717 0.738 0.728 0.606
    1.25 0.402 0.398 0.400 0.278
    0.625 0.281 0.278 0.280 0.158
    0.312 0.183 0.179 0.181 0.059
    0 0.123 0.120 0.122 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Scavenger receptor that displays several functions associated with host defense. Promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. Mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. Binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). May also play a role in the clearance of amyloid-beta in Alzheimer disease.
  • 基因功能参考文献：
    1. CL-P1 is a novel receptor involved in myelin uptake by phagocytes and likely plays a role in multiple sclerosis lesion development. PMID: 28317919
    2. rs17684886 (ZNRF1) and rs599019 (COLEC12) are associated with diabetic retinopathy and rs6427247 (SCYL1BP1) and rs899036 (API5) are associated with severe diabetic retinopathy in Chinese patients with type 2 diabetes PMID: 25819896
    3. The common presence of Lewis(x) groups in granule protein glycans can thus target granule proteins for clearance by SRCL. PMID: 21561871
    4. The Le(x) residues of both, CEACAM1 and CEA, interact with the human Le(x)-binding glycan receptors DC-SIGN and SRCL. PMID: 20034698
    5. haplotype structure of the CL-P1 gene obtained from six single-nucleotide polymorphisms that were genotyped with 108 alleles in Japanese subjects PMID: 12601552
    6. SRCL is expressed in some but not all nurse-like cells; its C-type lectin domain binds specifically to several carbohydrates including GalNAc, T and Tn antigen in a Ca2+-dependent manner. PMID: 12761161
    7. SRCL might be involved in selective clearance of specific desialylated glycoproteins from circulation and/or interaction of cells bearing Lewis(x)-type structures with the vascular endothelium PMID: 15845541
    8. CL-P1 is not a common membrane protein on endothelial cells found in normal tissues under steady state conditions. PMID: 18423602
    9. CL-P1 predominantly mediated phagocytosis for fungi in vascular endothelia. PMID: 19073604

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  • 亚细胞定位：
    Membrane; Single-pass type II membrane protein. Note=Forms clusters on the cell surface.
  • 组织特异性：
    Expressed in perivascular macrophages. Expressed in plaques-surrounding reactive astrocytes and in perivascular astrocytes associated with cerebral amyloid angiopathy (CAA) in the temporal cortex of Alzheimer patient (at protein level). Strongly expressed
  • 数据库链接：

    HGNC: 16016

    OMIM: 607621

    KEGG: hsa:81035

    STRING: 9606.ENSP00000383115

    UniGene: Hs.464422