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  4. Human Centromere protein K(CENPK) ELISA kit

Human Centromere protein K(CENPK) ELISA kit

  • 中文名称：
    人着丝粒蛋白K(CENPK)酶联免疫试剂盒
  • 货号：
    CSB-EL005214HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CENPK ELISA Kit was designed for the quantitative measurement of Human CENPK protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    CENPK ELISA Kit; ICEN37 ELISA Kit; FKSG14 ELISA Kit; Centromere protein K ELISA Kit; CENP-K ELISA Kit; Interphase centromere complex protein 37 ELISA Kit; Protein AF-5alpha ELISA Kit; p33 ELISA Kit
  • 缩写：
    CENPK
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CENPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %88-97
    1:2Average %100
    Range %90-105
    1:4Average %92
    Range %85-96
    1:8Average %92
    Range %84-96
  • 回收率：
    The recovery of human CENPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-95
    EDTA plasma (n=4)9990-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.889 1.966 1.928 1.798
    7501.426 1.476 1.451 1.321
    3750.921 0.900 0.911 0.781
    187.50.566 0.543 0.555 0.425
    940.369 0.345 0.357 0.227
    470.259 0.263 0.261 0.131
    23.50.199 0.192 0.196 0.066
    00.131 0.129 0.130
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
  • 相关疾病：
    Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with KMT2A/MLL1.
  • 亚细胞定位：
    Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.
  • 蛋白家族：
    CENP-K/MCM22 family
  • 组织特异性：
    Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.
  • 数据库链接：

    HGNC: 29479

    OMIM: 611502

    KEGG: hsa:64105

    STRING: 9606.ENSP00000242872

    UniGene: Hs.529778