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  4. Human Carboxypeptidase N catalytic chain(CPN1) ELISA kit

Human Carboxypeptidase N catalytic chain(CPN1) ELISA kit

  • 中文名称：
    人羧基肽酶N催化链(CPN1)酶联免疫试剂盒
  • 货号：
    CSB-EL005898HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CPN1 ELISA Kit was designed for the quantitative measurement of Human CPN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 mU/mL-2000 mU/mL and the sensitivity is 7.8 mU/mL.

  • 别名：
    ACBP ELISA Kit; Anaphylatoxin inactivator ELISA Kit; Arginine carboxypeptidase ELISA Kit; Carboxypeptidase N catalytic chain ELISA Kit; Carboxypeptidase N polypeptide 1 50 kD ELISA Kit; Carboxypeptidase N polypeptide 1 ELISA Kit; Carboxypeptidase N small subunit ELISA Kit; Carboxypeptidase N subunit 1 ELISA Kit; CBPN_HUMAN ELISA Kit; CPN ELISA Kit; CPN1 ELISA Kit; Kininase 1 ELISA Kit; Kininase-1 ELISA Kit; Kininase1 ELISA Kit; Lysine carboxypeptidase ELISA Kit; Plasma carboxypeptidase B ELISA Kit; SCPN ELISA Kit; Serum carboxypeptidase N ELISA Kit
  • 缩写：
    CPN1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 mU/mL-2000 mU/mL
  • 灵敏度：
    7.8 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CPN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 85-95    
    1:2 Average % 105    
    Range % 101-109    
    1:4 Average % 87    
    Range % 84-92    
    1:8 Average % 95    
    Range % 91-99    
  • 回收率：
    The recovery of human CPN1 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 98 93-103    
    EDTA plasma (n=4) 92 85-97    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    mU/ml OD1 OD2 Average Corrected    
    2000 1.998 2.112 2.055 1.916    
    1000 1.547 1.675 1.611 1.472    
    500 1.122 1.132 1.127 0.988    
    250 0.683 0.688 0.686 0.547    
    125 0.436 0.454 0.445 0.306    
    62.5 0.321 0.325 0.323 0.184    
    31.25 0.232 0.241 0.237 0.098    
    0 0.138 0.140 0.139 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
  • 基因功能参考文献：
    1. CPN binds to fibrinogen and is present in a fibrin clot prepared from plasma. PMID: 23000409
    2. carboxypeptidase N regulates the biologic activity of SDF-1alpha by reducing the chemokine-specific activity PMID: 15718415
    3. This review summarizes the structure, enzymatic properties and function of this enzyme, including insights gained by the recent elucidation of the crystal structure of the CPN catalytic subunit and structural modeling of the non-catalytic 83 kDa subunit. PMID: 18039526
  • 相关疾病：
    Carboxypeptidase N deficiency (CPND)
  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase M14 family
  • 组织特异性：
    Synthesized in the liver and secreted in plasma.
  • 数据库链接：

    HGNC: 2312

    OMIM: 212070

    KEGG: hsa:1369

    STRING: 9606.ENSP00000359446

    UniGene: Hs.2246