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  4. Human B1 bradykinin receptor(BDKRB1) ELISA kit

Human B1 bradykinin receptor(BDKRB1) ELISA kit

  • 中文名称：
    人B1缓激肽受体(BDKRB1)酶联免疫试剂盒
  • 货号：
    CSB-EL002651HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human BDKRB1 ELISA Kit was designed for the quantitative measurement of Human BDKRB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名：
    BDKRB1; BRADYB1; B1 bradykinin receptor; B1R; BK-1 receptor
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    25 pg/mL-1600 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BDKRB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 102
    Range % 99-107
    1:2 Average % 97
    Range % 91-103
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 104
    Range % 96-108
  • 回收率：
    The recovery of human BDKRB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 89-98
    EDTA plasma (n=4) 92 87-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 1.774 1.845 1.810 1.624
    800 1.321 1.428 1.375 1.189
    400 1.015 0.982 0.999 0.813
    200 0.712 0.701 0.707 0.521
    100 0.453 0.469 0.461 0.275
    50 0.311 0.324 0.318 0.132
    25 0.242 0.249 0.246 0.060
    0 0.185 0.187 0.186 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
  • 基因功能参考文献：
    1. The findings revealed that serum B1R levels may provide prognostic information and currently act as potential indicators for progression in atherosclerosis. PMID: 28990089
    2. Data show that mRNA and protein of bradykinin type 2 receptors, but not bradykinin type 1 receptors, were abundant in cultured c-Kit+ progenitor cells. PMID: 28099911
    3. promotes adhesion of neutrophils to endothelial cells PMID: 24728914
    4. kB1R heterodimerizes with kB2Rs in co-transfected HEK293 cells and natively expressing endothelial cells, resulting in significant internalization and desensitization of the kB1R response in cells pre-treated with kB2R agonist. PMID: 25289859
    5. BKR1 and BKR2 gene expression on peripheral monocytes is upregulated in essential hypertension PMID: 24401952
    6. B1 receptor-mediated vasodilatation in myometrial vessels absent in pre-eclampsia PMID: 24304135
    7. B1R agonist acts as a functional stimulus for the secretion of KLK1 and KLK6, an event relevant for kinin production and cell invasion, respectively PMID: 25503118
    8. Bradykinin B1 receptor signaling depends on receptor endocytosis. PMID: 23934212
    9. APJ and B1R can form heterodimers in transfected HEK293 cells and activations of APJ and B1R could up-regulate eNOS phosphorylation PMID: 24686079
    10. investigated if expression of B1 and B2 kinin receptors can be affected by IL-4 and IL-13; data show, for the first time, that IL-4 and IL-13 decrease kinin receptors in a STAT6-dependent mechanism PMID: 23351078
    11. A novel B1R splice variant and promoter regulatory elements determine tissue-specific B1R expression. PMID: 24475248
    12. the relevance of kinin B1 and B2 receptors in bladder cancer, was investigated. PMID: 23224295
    13. CPM binding to extracellular loop 2 of the B1R results in positive allosteric modulation of B1R signaling, and disruption of this interaction could provide a novel therapeutic approach to reduce pathological B1R signaling. PMID: 24108126
    14. This review focuses on airway MAPK/NFkappaB-dependent kinin receptor upregulation that links the environmental risk factors to airway hyperreactivity and airway inflammation. PMID: 23428345
    15. Kinin B1 receptor homo-oligomerization is required for receptor trafficking to the cell surface PMID: 23201435
    16. Bradykinin B1 receptor is induced in inflammatory diseases and contributes to hyperthermia through a vagal sensory mechanism. PMID: 22971439
    17. A peptidase-resistant BR agonist had no significant direct or indirect acute effect (4h) on BR expression. PMID: 21986309
    18. The transcriptional activity of the B1 receptor for kinins increased in patients with grade 1 and grade 2 endometrial cancer when compared to the control group, whereas it decreased in patients with grade 3 endometrial cancer. PMID: 22706224
    19. bradykinin B1 is inhibited by chromomenones PMID: 22088753
    20. The up-regulation of HER2 and B(1)R in precursor lesions of gallbladder carcinoma suggests cross-talk between these two receptors that may be of importance in the modulation of cell proliferation in gallbladder carcinogenesis. PMID: 22468084
    21. Taken together, this study demonstrates that Pseudomonas aeruginosa is capable of up-regulating human B1R expression via the NF-kappaB signaling pathway. PMID: 22092568
    22. Comparing phospholipase C beta activity and Ca(2+)-regulated signals, a temperature-dependent increase was only observed for bradykinin B(1) but not for bradykinin(2)receptor activation. PMID: 21871009
    23. Remote ischemic preconditioning decreases expression of kinin receptors on circulating human neutrophils. PMID: 20189583
    24. CPM and B1Rs on cell membranes form a critical complex that potentiates B1R signaling. PMID: 21454694
    25. Data suggest that B1R inhibition can reduce BBB damage and cell invasion during autoimmune CNS disease and may offer a novel anti-inflammatory strategy for the treatment of MS. PMID: 21216565
    26. Release of metalloproteases-2 and -9 is blocked after silencing of the kinin B1 receptor with a siRNA. PMID: 21147512
    27. Severe inflammation characterizes rapidly progressive glomerulonephritides, and expression of the kinin B1 receptor (B1R) associates with inflammation. PMID: 20448019
    28. The up-regulation of B1 receptors may contribute to acute inflammatory pain through TRPV1 activation. PMID: 20152050
    29. human chondrosarcoma tissues had significantly higher expression of the B1 and B2 receptors comparing to normal cartilage PMID: 19885862
    30. Laminar shear stress is a major determinant of functional B1R expression in endothelium. PMID: 19661485
    31. B(1)R-epidermal growth factor receptor crosstalk may be a key interaction that maintains tumor growth PMID: 19184415
    32. both B1 receptor and gC1q receptor are involved in the vascular leakage induced by hereditary and acquired angioedema plasma. PMID: 19796797
    33. B1 and B2 receptor expression was enhanced in tumor cells and tissue adjacent to gastric cancer compared with gastric ulcers. PMID: 11710536
    34. Novel mode of action of angiotensin I converting enzyme inhibitors: direct activation of bradykinin B1 receptor PMID: 11880373
    35. Bradykinin B1 receptors are upregulated by inflammatory stimuli in bronchial epithelial cells. PMID: 12063092
    36. the C-terminal domain participates intimately in the efficacy of B1R and B2R G(q/11) coupling by contributing both positive and negative regulatory epitopes. PMID: 12130679
    37. Allergic rhinitis subjects displayed significantly higher expression of B1 receptor mRNA than did the normal subjects. PMID: 12165532
    38. B1R exists in caveolae-related lipid rafts (CLRs) in HEK293 cells apparently by default, as a result of their random distribution in the plasma membrane and not by being targeted to the CLR fraction. PMID: 12450400
    39. B1 and B2 bradykinin receptors form a complex with enhanced signaling capacity PMID: 15033977
    40. Bradykinin B(1) receptor homooligomerization is required for expression of receptors on the cell surface and subsequent constitutive receptor signaling. PMID: 15492119
    41. kinin B(1) receptors participate in painful and inflammatory disorders--REVIEW PMID: 15520046
    42. slow internalization of B(1)KB(2) was also accompanied by a lack of agonist-induced phosphorylation, that in contrast was observed for B(1)YB(2) and B(1)CB(2), suggesting that putative helix 8 is either directly or indirectly involved PMID: 15634338
    43. the two bradykinin receptors may play a role in blood pressure regulation PMID: 15643125
    44. The B1 kinin receptor does not have a major vasomotor or fibrinolytic role in patients with heart failure PMID: 15681300
    45. Use of the highly sensitive DNase I in vivo footprinting approach to delineate more precisely the functional domains of the BDKRB1 gene promoter in human smooth muscle cells is reported. PMID: 15705059
    46. bradykinin receptors in imflammatory bowel disease may reflect intestinal inflammation PMID: 15805101
    47. A novel class of 2,3-diaminopyridine bradykinin B1 receptor antagonists is disclosed using structure-activity relationship studies. PMID: 15837330
    48. BK-1Rs are induced in the endothelium of intramyocardial coronary vessels in failing human hearts and so may participate in the pathogenesis of heart failure. PMID: 15840906
    49. Kinin B1 receptor expression on multiple sclerosis mononuclear cells PMID: 15883268
    50. Increased level of bradykinin receptor B1 in adenoma suggests that kinins may play a role in abnormal cellular transformation. PMID: 16644486

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Bradykinin receptor subfamily, BDKRB1 sub-subfamily
  • 数据库链接：

    HGNC: 1029

    OMIM: 600337

    KEGG: hsa:623

    STRING: 9606.ENSP00000216629

    UniGene: Hs.525572