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  4. Human Angiopoietin-related protein 7(ANGPTL7) ELISA kit

Human Angiopoietin-related protein 7(ANGPTL7) ELISA kit

  • 中文名称：
    人血管生成素相关蛋白7(ANGPTL7)酶联免疫试剂盒
  • 货号：
    CSB-EL001715HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ANGPTL7 ELISA Kit was designed for the quantitative measurement of Human ANGPTL7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    Angiopoietin like 7 ELISA Kit; Angiopoietin like factor (CDT6) ELISA Kit; Angiopoietin like factor ELISA Kit; Angiopoietin related protein 7 ELISA Kit; Angiopoietin-like factor ELISA Kit; Angiopoietin-like protein 7 ELISA Kit; Angiopoietin-related protein 7 ELISA Kit; ANGL7_HUMAN ELISA Kit; ANGPTL7 ELISA Kit; AngX ELISA Kit; CDT6 ELISA Kit; Cornea derived transcript 6 protein ELISA Kit; Cornea-derived transcript 6 protein ELISA Kit; dJ647M16.1 ELISA Kit; OTTHUMP00000001986 ELISA Kit; RP4-647M16.2 ELISA Kit
  • 缩写：
    ANGPTL7
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 99
    Range % 92-104
    1:2 Average % 87
    Range % 82-93
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 101
    Range % 98-105
  • 回收率：
    The recovery of human ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 92-106
    EDTA plasma (n=4) 95 89-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 1.942 2.042 1.992 1.881
    10 1.239 1.149 1.194 1.083
    5 0.759 0.719 0.739 0.628
    2.5 0.453 0.464 0.459 0.348
    1.25 0.311 0.321 0.316 0.205
    0.625 0.212 0.223 0.218 0.107
    0.312 0.161 0.165 0.163 0.052
    0 0.110 0.111 0.111 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure. Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency.
  • 基因功能参考文献：
    1. obesity increases the level of ANGPTL7 in both plasma and adipose tissue. Increased expression of ANGPTL7 might play a minor role in the regulation of TG level in obese subjects either directly or through interaction with other ANGPTL protein members. Physical exercise reduced the level of ANGPTL7 highlighting the potential for targeting this protein as a therapeutic target for regulating dyslipidemia. PMID: 28264047
    2. ANGPTL7, which is abundantly expressed in keratocytes, plays a major role in maintaining corneal avascularity and transparency. PMID: 25622036
    3. ANGPTL7 is markedly down regulated in metastatic liver cancer. PMID: 25854806
    4. Angiopoietin-like 7 is a novel pro-angiogenetic factor over-expressed in cancer PMID: 24903490
    5. Together with previous findings, these properties strengthen ANGPTL7's candidacy for the regulation of intraocular pressure and glaucoma. PMID: 21199193
    6. Induction of ANGPTL7 secretion by glaucoma stimuli and increased concentration of ANGPTL7 in glaucomatous AH suggest that ANGPTL7 is overexpressed in glaucoma. PMID: 18421092

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Higly expressed in the cornea (at protein level). Expression is restricted to the stromal layer. Also detected at the junction between the corneal stromal layer and the conjuctiva. Not detected in the sclera.
  • 数据库链接：

    HGNC: 24078

    KEGG: hsa:10218

    STRING: 9606.ENSP00000366015

    UniGene: Hs.146559